30results about How to "Complete three-dimensional structure" patented technology

The invention discloses a carboxymethylcellulose (CMC) nanofiber membrane loaded with silver nanoparticles and a preparation method thereof and aims at providing a silver-loaded antibacterial nanofiber and a preparation method which have the characteristics that the preparation method is simple and pollution-free, the prepared fiber membrane has excellent antibacterial property and biocompatibility and only CMC serves as a base material. The preparation method comprises the following steps: performing electrostatic spinning by taking a mixed solution of polyethylene oxide (PEO) and CMC as a spinning solution; soaking a fiber membrane in a silver nitrate solution of a certain concentration, taking out the membrane, drying in the dark, and reducing in moist air at normal temperature; and finally, preparing the CMC nanofiber membrane loaded with the silver nanoparticles. The prepared fiber membrane has excellent antibacterial property and biocompatibility, is good in structure and mechanical property retention and is a preferred material in the field of wound coating, and the preparation method is simple, economic and environment-friendly.

The invention belongs to the field of medical repair materials and relates to a method for preparing a cell-free medical implant material. In the method for preparing the cell-free materials, neitherchemically synthesized detergents nor protease products are used to remove cells; but a plant-derived nonionic surfactant is used as a decellularizing agent, thus avoiding the damage of chemical detergents, protease preparations and the like to an ECM three-dimensional structure and the loss of effective active ingredients in ECM. The cell-free medical material prepared by the method can not onlybetter maintain the three-dimensional structure of ECM, but also allow more effective active ingredients to be remained in ECM and endowed with good mechanical properties. Thereby, the ability to induce chemotaxis and differentiation of host cells is stronger, and the effect of promoting tissue repair and regeneration will be better.

The invention relates to enzyme method measuring zinc ion concentration and zinc ion diagnose kit. The invention uses alkaline phosphatase reaction colorimetry method that uses alkaline phosphatase to enzymolysis four-nitrophenol phosphoric acid to form into four-nitrophenol under the activation of zinc ion, by detecting the ascending speed of 405nm dominant wavelength absorbance the zinc ionic content in sample would be quantitatively responsed. The method has high specificity, has no influence from inside and outside metal material, accurate test result. The diagnose kit is made up into bi-agent or tri-agent that could reduce cross influence from each component and keeps stability of the agent that is convenient for long-term storage. The method could quickly test on commonness ultraviolet / visible light, analyzer or semi-transfer / full automatic biochemical study instrument, and doesn't need particularity or extra instrument. The testing cost is cheap, and is convenient for popularization.

The invention is about the enzyme method of measuring magnesium ion and its diagnosis reagent box. Making use of the peculiarity that magnesium ion in the sample of plasma or serum and so on can activate the activation of glycerokinase, and producing glycerol-3-phosphoryl by reacting glycerol under the existence of adenosine triphosphate, and then causing coupled reaction with glycerol-3-phosphoryl dehydrogenase and transferring oxidized coenzyme to reduced coenzyme. Testing the descending range of dominant wave-length340nm absorbance and finally measuring the content of magnesium ion in the sample. This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet / visible light analyzer or semiautomatic / automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.

A method for determining inorganic phosphorus includes utilizing glyceraldehyde - 3 - phosphate dehydrogenase to react with glyceraldehyde - 3 - phosphate under activation of inorganic phosphorus in serum sample to make oxidized coenzyme be reduced coenzyme then detecting rise amplitude of absorbance at main wavelength of 340nm before and after reaction. A diagnostic kit is also disclosed.

The invention belongs to the technical field of metal coating detection, and provides a method for displaying the three-dimensional structure of a silicon-rich phase in an aluminum-silicon coating by a metallographic method, including sample preparation, chemical corrosion and microscope observation. Coated steel plate, cut a part of it as a sample, and then inlaid and polished the sample in turn; (2) Chemical corrosion: put the sample prepared in step (1) into a corrosion solution for chemical corrosion, and the corrosion solution is made of an aqueous hydrochloric acid solution. (3) Microscope observation: After the chemical corrosion is completed, rinse the sample, dry it, and observe it with a ZISS metallographic microscope. The method provided by the invention can obtain a complete and clear three-dimensional structure of the silicon-rich phase, can fully display the growth of the silicon-rich phase, and has the characteristics of low detection cost, rapidity and simplicity.

The invention belongs to the biology and pharmaceutical technical field, and relates to oral mixed nucleoside and the preparation process thereof. The invention mainly provides the oral mixed nucleoside and the preparation process thereof in order to overcome the problems existing in the prior art that the spatial structure of the product is damaged to a certain degree, the production cycle is longer, the operation is difficult, and the integrated cost is high. In order to overcome the problems existing in the prior art, the invention has the technical proposal that the oral mixed nucleoside is obtained through the following preparation processes, and the preparation processes comprise the steps that step one, malt roots are prepared; step two, pre-treatment: the malt roots are extracted through water, and filtered, after being preheated, phosphatase liquid is added to be prepared to be used; step three, hydrolytic reaction: purified water is preheated, and ribonucleic acid is added into the purified water, the pH value is adjusted, the mixed fluid in the step two is added into the purified water, the pH value is adjusted again, and the purified water is insulated and reacts; stepfour, the fluid is inactivated, the pH value is adjusted, and the fluid is filtered; step five, the fluid is concentrated and dried.

The invention discloses a carboxymethyl cellulose (CMC) nanofiber membrane loaded with silver nanoparticles and a preparation method thereof. The purpose is to provide a simple and pollution-free preparation method, and the prepared fiber membrane has excellent antibacterial properties and Biocompatible silver-loaded antibacterial nanofiber with only CMC as substrate and preparation method thereof. In the present invention, the mixed solution of polyethylene oxide (PEO) and CMC is used as the spinning stock solution to carry out electrospinning, and then the fiber film is soaked in a silver nitrate solution of a certain concentration, taken out and dried in the dark and placed in the The reduction is carried out in humid air at room temperature, and finally a nanofiber membrane of CMC nanofibers loaded with silver nanoparticles is prepared. The fiber membrane prepared by the invention has excellent antibacterial properties and biocompatibility, and the structure and mechanical properties of the fiber membrane itself are well maintained, and is a preferred material in the field of wound dressing, and the preparation method is simple, economical and environment-friendly.

The invention is about the measuring method of Inorganic Phosphates and its diagnosis reagent box. Producing hypoxanthine by reacting nucleoside phosphorylase with carnine under the existence of Inorganic Phosphates , then producing hydroperoxide by reactinghypoxanthine with xanthine oxidase, and then reacting hydroperoxide with peroxidaseand oxidating the colorless reduced chromogen combination to quinoneimine chromogen or indamide chromogen dyer with color, testing the variation of dominant wave-length400ú¡600nmabsorbance during the reaction and finally measuring the content of Inorganic Phosphates . This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet / visible light analyzer or semiautomatic / automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.