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Low-temperature crude extraction process of squid ink antioxidant substances

A technology of antioxidant substances and ink, which is applied in the direction of anti-toxic agents, food science, drug combination, etc., can solve the problems such as the extraction process of antioxidant substances in squid ink that have not yet been seen, and achieve the advantages of easy magnetic separation, low oxidation rate of products, and high content Effect

Active Publication Date: 2019-12-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a large number of active substances such as squid ink proteoglycan and peptidoglycan have been studied, but there are no reports on the extraction process of squid ink antioxidant substances

Method used

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  • Low-temperature crude extraction process of squid ink antioxidant substances
  • Low-temperature crude extraction process of squid ink antioxidant substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of magnetically immobilized compound enzyme:

[0027] S1. Preparation of magnetic nanoparticles: 10g of ferric chloride hexahydrate and 20g of ferrous chloride tetrahydrate were heated up to the reaction temperature in a nitrogen atmosphere, and then 100mL of 20wt% ammonia water was added dropwise. to room temperature, the synthesized magnetic nanoparticles were washed several times with deionized water, separated by a magnet, and set aside;

[0028] S2. Preparation of immobilized compound enzyme: adjust food-grade lipase and food-grade papain with sterile water to respectively adjust the concentrated solution, mix equal volumes, add sodium alginate and mix evenly, so that the sodium alginate is 5wt%, use The syringe draws the mixed solution, and 3.5wt% CaCl is added drop by drop 2 Solution, prepare immobilized microspheres, after cross-linking for 12 hours, wash with sterile water, grind to 10-100nm, and set aside;

[0029] S3. Preparation of m...

Embodiment 2

[0032] The preparation method of magnetically immobilized compound enzyme:

[0033] S1. Preparation of magnetic nanoparticles: 10g of ferric chloride hexahydrate and 30g of ferrous chloride tetrahydrate were heated to the reaction temperature in a nitrogen atmosphere, and then 300mL of 24wt% ammonia water was added dropwise, and reacted at a constant temperature of 60°C under nitrogen protection for 2-5h , down to room temperature, the synthesized magnetic nanoparticles were washed with deionized water several times, separated by a magnet, and set aside;

[0034] S2. Preparation of immobilized compound enzyme: adjust food-grade lipase and food-grade papain with sterile water to respectively adjust the concentrated solution, mix equal volumes, add sodium alginate and mix evenly, so that the sodium alginate is 5wt%, use The syringe draws the mixed solution, and 3.5wt% CaCl is added drop by drop 2 Solution, prepare immobilized microspheres, after cross-linking for 12 hours, wash...

Embodiment 3

[0038]The preparation method of magnetically immobilized compound enzyme:

[0039] S1. Preparation of magnetic nanoparticles: 10g of ferric chloride hexahydrate and 25g of ferrous chloride tetrahydrate were heated to the reaction temperature in a nitrogen atmosphere, and 200mL of 22wt% ammonia water was added dropwise, and reacted at a constant temperature of 55°C under nitrogen protection for 3.5h. Cool down to room temperature, wash the synthesized magnetic nanoparticles with deionized water several times, separate with a magnet, and set aside;

[0040] S2. Preparation of immobilized compound enzyme: adjust food-grade lipase and food-grade papain with sterile water to respectively adjust the concentrated solution, mix equal volumes, add sodium alginate and mix evenly, so that the sodium alginate is 5wt%, use The syringe draws the mixed solution, and 3.5wt% CaCl is added drop by drop 2 Solution, prepare immobilized microspheres, after cross-linking for 12 hours, wash with st...

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Abstract

The invention provides a low-temperature crude extraction process of squid ink antioxidant substances. Squid ink is unfrozen, distilled water is added, stirring and washing are carried out, impuritiesare removed by using coarse filter cloth, then a squid viscera autolysis solution is added, autolyzing is carried out for 1-2 hours, then enzyme deactivating and filtering are performed, the pH valueis regulated by using alkali liquor, magnetic immobilized compound enzyme is added while stirring for enzymolysis, circular stirring is carried out, the enzyme is made to make contact with a substrate fully, and reaction is ended when the magnetic immobilized compound enzyme is removed by using a magnet; the pH value is regulated with an acid solution, centrifuging is performed, and the precipitate is collected; and obtained precipitate is repeatedly washed with distilled water and centrifuged until washing water is neutral, vacuum freeze drying is carried out, and a finished product is stored at -20 DEG C. The used raw materials are rich and easy to obtain, the enzyme method immobilization technology can be repeatedly used, the cost is reduced, the oxidation rate of the product is reduced through low-temperature treatment, the content of antioxidant melanin and ink polysaccharide is extremely high, and the substances can be used for preparing antioxidant health-care food or medicine.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a low-temperature crude extraction process of antioxidant substances in squid ink. Background technique [0002] In recent years, with the development of my country's ocean-going aquatic product fishing industry, the total amount of squid caught and international squid processing has reached hundreds of thousands of tons per year, and the processing waste squid ink sac is 1.28% of the squid's weight. The amount is considerable. According to literature reports, the squid ink antioxidant substances in the ink sac can resist oxidation, improve the immunity of patients and have strong anti-radiation effects. [0003] Squid is a marine animal of Mollusca, Cephalopoda, and Calamariidae. According to reports, the annual catch of squid in the world reaches 4 million tons, and my country is one of the countries with the highest catch in the world. During squid processing, most processing pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/618A61P39/06A23L17/50A23L17/20A23L29/00A23L33/00
CPCA61K35/618A61P39/06A23L17/50A23L17/20A23L29/06A23L33/00A23V2002/00A23V2200/30A23V2250/2042
Inventor 郭健宋文东蔡璐纪丽丽王亚宁王振
Owner ZHEJIANG OCEAN UNIV