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Method for extracting perillaldehyde from perilla

A technology of perilla aldehyde and perilla, which is applied in the field of extraction, can solve the problems of complex chemical synthesis process, reduce the content of perillaldehyde, and the extraction effect is not good enough, so as to prolong the storage time, improve the quality and utilization rate, and improve the purity Effect

Inactive Publication Date: 2019-12-20
贵州侗乡生态农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process of chemical synthesis is complicated, and a variety of harmful chemical reagents are introduced and left in the reaction process, which greatly limits its application in the pharmaceutical and food industries
In the existing literature, perilla aldehyde is directly extracted from plants using water bath ultrasonic method, Soxhlet extraction method and heating reflux method (see Geng Ronghui, Yang Yujie, Hu Chunlan, etc. "Extraction and content of perilla aldehyde in perilla leaves Determination "[J]. Chinese Contemporary Medicine, 2014, (7): 4-7.), the extraction effect is not good enough, and the yield of perillaldehyde obtained by the heating reflux method with the highest efficiency is about 13% (see CN201810982875-1 The extraction method of perillaldehyde), the extraction is not enough
And most of the prior art adopts the method of drying and pulverizing perilla, which will reduce the content of perillaldehyde to a certain extent, and the single extraction method is incomplete, and the purification of perilla aldehyde after the extraction is mostly done by evaporation and concentration , the obtained perillaldehyde contains many impurities, and the purity is not high enough

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  • Method for extracting perillaldehyde from perilla
  • Method for extracting perillaldehyde from perilla
  • Method for extracting perillaldehyde from perilla

Examples

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Embodiment 1

[0034] A method for extracting perillaldehyde from perilla, comprising the following steps:

[0035] (1) Pretreatment: Wash the perilla leaves and perilla calyxes in the flowering stage with water and grind to extract the juice, take the perilla juice, soak the remaining perilla residue with ethyl acetate with a material-to-liquid ratio of 1:3 for 2 hours, Take the soaking liquid and mix it with perilla juice, add 2% borax solution to the water-soluble rosmarinic acid, grind it thoroughly, evaporate and concentrate to half the volume of the previous solution, and then add the treated antioxidant to the perilla juice;

[0036] (2) Acidification and enzymolysis: first cellulase: pectinase: xylanase: xylosidase is made into compound enzyme, then trichoderma, actinomycetes and Bacillus sp. After mixing at a ratio of %, add 1.5mol / L glucose solution to make a complex microbial flora, then add the complex microbial flora and stir for 3 hours at a speed of 50rpm, add citric acid to t...

Embodiment 2

[0049] A method for extracting perillaldehyde from perilla, comprising the following steps:

[0050] (1) Pretreatment: Wash the perilla leaves and perilla calyxes in the flowering stage with water and grind to extract the juice, take the perilla juice, soak the remaining perilla residue with ethyl acetate with a material-to-liquid ratio of 1:3 for 2 hours, Take the soaking liquid and mix it with perilla juice, add 2% borax solution to the water-soluble rosmarinic acid, grind it thoroughly, evaporate and concentrate to half the volume of the previous solution, and then add the treated antioxidant to the perilla juice;

[0051] (2) Acidification and enzymolysis: first cellulase: pectinase: xylanase: xylosidase is made into compound enzyme, then trichoderma, actinomycetes and Bacillus sp. After mixing at a ratio of %, add 2.5mol / L glucose solution to make a complex microbial flora, then add the complex microbial flora and stir for 3 hours at a speed of 60rpm, add citric acid to t...

Embodiment 3

[0064] A method for extracting perillaldehyde from perilla, comprising the following steps:

[0065] (1) Pretreatment: wash the perilla leaves and perilla calyxes in the flowering stage with water and grind to extract the juice, take the perilla juice, soak the remaining perilla residue with ethyl acetate with a material-to-liquid ratio of 1:3 for 2 hours, Take the soaking liquid and mix it with perilla juice, add 2% borax solution to the water-soluble rosmarinic acid, grind it thoroughly, evaporate and concentrate to half the volume of the previous solution, and then add the treated antioxidant to the perilla juice;

[0066] (2) Acidification and enzymolysis: first cellulase: pectinase: xylanase: xylosidase is made into compound enzyme, then trichoderma, actinomycetes and Bacillus sp. After mixing at a ratio of %, add 1.5mol / L glucose solution to make a complex microbial flora, then add the complex microbial flora and stir for 3 hours at a speed of 70rpm, add citric acid to t...

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Abstract

The invention belongs to the technical field of extraction, and particularly relates to a method for extracting perillaldehyde from perilla. The method comprises the following steps: pretreatment, acidification and enzymolysis, primary treatment of an enzyme, secondary treatment, extraction, decolorization and impurity removal, vacuum distillation, and deodorization and purification, an extractionrate is up to 24.76%, the purity is up to 99.3% or more, and quality and a utilization rate of perillaldehyde are significantly improved. The activity of the enzyme is improved through pretreatment,the enzyme has relatively high efficiency in a process of enzymatic destruction of cell walls, enzymolysis is relatively complete, the extraction rate is improved while chemical properties of perillaldehyde is maintained to the maximum, introduction and residue of organic reagents are greatly reduced, and thereby purity and quality of the perillaldehyde finished-product are improved.

Description

technical field [0001] The invention belongs to the technical field of extraction, in particular to a method for extracting perillaldehyde from perilla. Background technique [0002] Perilla is an annual herbaceous plant of the genus Perilla in the family Lamiaceae, also known as Guiren, Baisu, Chisu, etc. Its leaves, stems, and seeds can be used as medicine. Perilla volatile oil is the main active ingredient of perilla leaves, which is widely used in medicine, food industry and other industries. Among them, perillaldehyde is the main component and characteristic aroma component of perilla volatile oil, which can be added to food, tobacco and cosmetics as spices. Pharmacological tests show that perillaldehyde has anti-aging, blood fat-lowering, anti-inflammation, anti-depression, antibacterial and anti-tumor functions, and after perillaldehyde is made into an oxime, the sweetness is 2000 times that of sugar, and it can raise blood sugar when taken orally. The effect is str...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C47/45C07C45/86C07C45/79
CPCC07C45/78C07C45/86C07C45/79C07C2601/16C07C47/45
Inventor 刘荣贵许志祥
Owner 贵州侗乡生态农业科技发展有限公司
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