Environment deodorization compound bacterium preparation and preparation method thereof
A compound bacterial agent and environmental technology, which is applied in the direction of biochemical equipment and methods, separation methods, chemical instruments and methods, etc., can solve the problems of poor late effect, low activity, loss of appetite of pigs, etc., and achieves strong practicability and improved The effect of immunity
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Embodiment 1
[0019] Step a: Incline cultivation:
[0020] The capsular photosynthetic bacteria, Rhodopseudomonas palustris, Rhodococcus rhodococcus, Bacillus subtilis, Lactobacillus plantarum, nitrifying bacteria, denitrifying bacteria, alcoholic yeast, Bacillus licheniformis, and Acetobacter were respectively added to the "beef extract peptone solid" The slant test tube culture of the mother species is carried out on the nutrient medium, the temperature is set at 32 degrees to 34 degrees, and the culture is carried out for 2 to 3 days for later use.
[0021] Step b: Seed culture:
[0022] 1: Under aseptic conditions, inoculate the parent species of photosynthetic bacteria, Rhodopseudomonas palustris and Rhodococcus rubrum cultivated in step a into liquid 500ml triangular flasks (special medium for photosynthetic bacteria, liquid volume 200ml) ) for cultivation, the temperature is set at 32°C to 34°C, and cultured in a shaker at 180r / min for 2-3 days;
[0023] Among them, the special liq...
Embodiment 2
[0027] Compound fermentation culture:
[0028] Step c: the ratio of bacteria types:
[0029] Mix the seed liquid in the triangular flask in step b in Example 1 according to the percentage by weight: 10% of Capsular Photosynthetic Bacteria; 5% of Rhodopseudomonas palustris; 8% of Rhodococcus rhodococcus; 10% of Bacillus subtilis; Lactobacillus plantarum 15%; Nitrifying bacteria 10%; Denitrifying bacteria 8%; Alcohol yeast 2%; Bacillus licheniformis 8%; Acetobacter 10%;
[0030] Step d: Composite culture medium for fermenter: take the raw materials with the following percentages to make culture medium and carry out sterilization treatment:
[0031] Molasses 2.0%; Glucose 1.0%; Potato powder 1.8%; Soybean meal powder (through 70 mesh sieve) 1.7%; Magnesium sulfate 0.04%; Paste 0.03%; the balance is water. Adjust the pH value to 7.2; sterilize for 30 minutes at a temperature of 121 degrees.
[0032]Step e: Insert the liquid seed liquid prepared in step c into a fermenter with ...
Embodiment 3
[0034] Compound fermentation culture:
[0035] Step c: the ratio of bacteria types:
[0036] Mix the seed liquid in the triangular flask in step b in Example 1 according to the percentage by weight: Capsular photosynthetic bacteria 15%; Rhodopseudomonas palustris 8%; Rhodococcus rhodococcus 10%; Bacillus subtilis 8%; Lactobacillus plantarum 12%; Nitrifying bacteria 6%; Denitrifying bacteria 6%; Alcohol yeast 3%; Bacillus licheniformis 12%; Acetobacter 6%;
[0037] Step d: Composite culture medium for fermenter: take the raw materials with the following percentages to make culture medium and carry out sterilization treatment:
[0038] Molasses 1.2%; Glucose 0.8%; Potato powder 1.6%; Soybean meal powder (through 70 mesh sieve) 1.5%; Magnesium sulfate 0.02%; Wood acetic acid 1.6%; Urea 0.1%; Potassium dihydrogen phosphate 0.06%; Paste 0.05%; the balance is water. Adjust to pH 7; sterilize for 30 minutes at a temperature of 121 degrees.
[0039] Step e: Insert the liquid seed ...
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