Environment deodorization compound bacterium preparation and preparation method thereof

A compound bacterial agent and environmental technology, which is applied in the direction of biochemical equipment and methods, separation methods, chemical instruments and methods, etc., can solve the problems of poor late effect, low activity, loss of appetite of pigs, etc., and achieves strong practicability and improved The effect of immunity

Active Publication Date: 2019-12-20
四川明湖环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It not only has a negative impact on the ecological environment, but also poses a serious threat to the healthy growth of livestock and poultry, harmless breeding and human health (when the ammonia in the air reaches 37.5mg / m 3 When the weight gain of live pigs is slow, it can reach 50mg / m 3 At this time, the feed intake of the piglets fed for 4 weeks decreased by 15.6%, and the feed utilization rate decreased by 18%; when the hydrogen sulfide in the air reached 30mg / m 3 Pigs become photophobia, loss of appetite, nervousness; when it reaches 75mg / m 3 Pigs will suddenly vomit, lose consciousness, and finally die due to paralysis of the respiratory center and motor center), which seriously restricts the sustainable development of my country's livestock and poultry industry
[0003] There are many "environmental deodorants" on the market, most of which use chemical aromatic substances to cover up the symptoms, not the root cause, and cause secondary environmental pollution
Although there are many "microbial deodorant preparations" in the market, their activity is not high, and the combination of complex bacteria is unreasonable, resulting in unsatisfactory "deodorant" effects, or the effect is effective in the early stage, but the effect in the later stage is poor.

Method used

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  • Environment deodorization compound bacterium preparation and preparation method thereof
  • Environment deodorization compound bacterium preparation and preparation method thereof
  • Environment deodorization compound bacterium preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step a: Incline cultivation:

[0020] The capsular photosynthetic bacteria, Rhodopseudomonas palustris, Rhodococcus rhodococcus, Bacillus subtilis, Lactobacillus plantarum, nitrifying bacteria, denitrifying bacteria, alcoholic yeast, Bacillus licheniformis, and Acetobacter were respectively added to the "beef extract peptone solid" The slant test tube culture of the mother species is carried out on the nutrient medium, the temperature is set at 32 degrees to 34 degrees, and the culture is carried out for 2 to 3 days for later use.

[0021] Step b: Seed culture:

[0022] 1: Under aseptic conditions, inoculate the parent species of photosynthetic bacteria, Rhodopseudomonas palustris and Rhodococcus rubrum cultivated in step a into liquid 500ml triangular flasks (special medium for photosynthetic bacteria, liquid volume 200ml) ) for cultivation, the temperature is set at 32°C to 34°C, and cultured in a shaker at 180r / min for 2-3 days;

[0023] Among them, the special liq...

Embodiment 2

[0027] Compound fermentation culture:

[0028] Step c: the ratio of bacteria types:

[0029] Mix the seed liquid in the triangular flask in step b in Example 1 according to the percentage by weight: 10% of Capsular Photosynthetic Bacteria; 5% of Rhodopseudomonas palustris; 8% of Rhodococcus rhodococcus; 10% of Bacillus subtilis; Lactobacillus plantarum 15%; Nitrifying bacteria 10%; Denitrifying bacteria 8%; Alcohol yeast 2%; Bacillus licheniformis 8%; Acetobacter 10%;

[0030] Step d: Composite culture medium for fermenter: take the raw materials with the following percentages to make culture medium and carry out sterilization treatment:

[0031] Molasses 2.0%; Glucose 1.0%; Potato powder 1.8%; Soybean meal powder (through 70 mesh sieve) 1.7%; Magnesium sulfate 0.04%; Paste 0.03%; the balance is water. Adjust the pH value to 7.2; sterilize for 30 minutes at a temperature of 121 degrees.

[0032]Step e: Insert the liquid seed liquid prepared in step c into a fermenter with ...

Embodiment 3

[0034] Compound fermentation culture:

[0035] Step c: the ratio of bacteria types:

[0036] Mix the seed liquid in the triangular flask in step b in Example 1 according to the percentage by weight: Capsular photosynthetic bacteria 15%; Rhodopseudomonas palustris 8%; Rhodococcus rhodococcus 10%; Bacillus subtilis 8%; Lactobacillus plantarum 12%; Nitrifying bacteria 6%; Denitrifying bacteria 6%; Alcohol yeast 3%; Bacillus licheniformis 12%; Acetobacter 6%;

[0037] Step d: Composite culture medium for fermenter: take the raw materials with the following percentages to make culture medium and carry out sterilization treatment:

[0038] Molasses 1.2%; Glucose 0.8%; Potato powder 1.6%; Soybean meal powder (through 70 mesh sieve) 1.5%; Magnesium sulfate 0.02%; Wood acetic acid 1.6%; Urea 0.1%; Potassium dihydrogen phosphate 0.06%; Paste 0.05%; the balance is water. Adjust to pH 7; sterilize for 30 minutes at a temperature of 121 degrees.

[0039] Step e: Insert the liquid seed ...

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Abstract

The invention relates to the field of microorganism environmental protection, and aims to provide an environment deodorization compound bacterium preparation and a preparation method thereof. The environment deodorization compound bacterium preparation comprises 8%-20% of capsule photosynthetic bacteria, 5%-10% of rhodop seudanonas palustris, 8%-12% of azolla rhodococcus, 6%-10% of bacillus subtilis, 8%-15% of lactobacillus plantarum, 3%-10% of nitrifying bacteria, 5%-8% of denitrifying bacteria, 2%-5% of alcohol yeast, 8%-15% of bacillus licheniformis and 5%-10% of acetobacter xylinum. According to the environment deodorization compound bacterium preparation, the strains are preferably selected for compatibility, and through composite fermentation, the strains are in complementary antagonism, can realize harmonious growth and are high in practicality. The immunity of livestock and poultry is improved. The compound strain prepared by the method is diluted into 50-100 times in volume, and then is prayed to the ground of a livestock and poultry bed, only about 20 minutes is needed, and harmful ammonia and hydrogen sulfide gas can be reduced from 18ppm or below from 100ppm. Secondarypollution is not caused.

Description

technical field [0001] The invention relates to the field of microbial environmental protection, in particular to a preparation method of an environment deodorizing composite bacterial agent. Background technique [0002] With the continuous adjustment and change of the agricultural structure, my country's livestock and poultry husbandry has developed rapidly in terms of breeding scale and quality; but at the same time, the environmental pollution caused by livestock and poultry breeding is very serious. Affected by factors such as breeding mode and breeding cost, pollutants such as sewage, feces, and feed residues produced in the livestock and poultry breeding process pile up like mountains and cannot be scientifically and centrally treated. The harmful gas of ammonia gas in livestock and poultry pens is as high as 50ppm-100ppm or more , some hydrogen sulfide gas is as high as 20ppm-50ppm or more. It not only has a negative impact on the ecological environment, but also po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18B01D53/84B01D53/52B01D53/58C12R1/125C12R1/25C12R1/10C12R1/02C12R1/865C12R1/01
CPCC12N1/20C12N1/18B01D53/84B01D53/52B01D53/58B01D2258/0266B01D2251/95Y02A50/20
Inventor 杨建设张元元
Owner 四川明湖环保科技有限公司
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