Saline-alkaline resistant Bacillus pacificus, and biogen and application thereof

A technology of bacillus and live bacteria preparations, applied in the field of agricultural biology, can solve problems such as limited control effects, achieve the effects of reducing disease occurrence, high colonization rate, and improving yield and quality

Active Publication Date: 2019-12-27
SHANDONG ZOETICLAND BIOLOGICAL TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, beneficial microorganisms such as Bacillus cereus, Trichoderma harzianum, Bacillus subtilis, Bacillus thuringiensis, Streptomyces lavender, Streptomyces griseus and other ben...

Method used

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  • Saline-alkaline resistant Bacillus pacificus, and biogen and application thereof
  • Saline-alkaline resistant Bacillus pacificus, and biogen and application thereof
  • Saline-alkaline resistant Bacillus pacificus, and biogen and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the screening of Pacific bacillus YJJK-16

[0020] (1) Screening of saline-alkali-tolerant strains

[0021] Bacillus pacificus YJJK-16 was isolated from cabbage rhizosphere soil. The soil was sampled in Dongying City, Shandong Province, which is a saline-alkali soil. The specific separation method is: weigh 10 g of soil sample, put it into a pre-sterilized 250 mL Erlenmeyer flask filled with 90 mL of deionized water and 10-20 glass beads, shake at 180 r / min for 20 min. Using the gradient dilution method, the soil solution was diluted to 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 . Take 0.1mL of soil solution with different dilutions, put it into a high-salt-alkaline selective medium plate, spread it evenly, and then incubate at 37°C for 24-48h. According to the difference in colony and cell morphology, different strains were transferred to the slant of the preservation medium test tube, cultured at 37°C for 1-2d, and after the bacterial lawn was cov...

Embodiment 2

[0027] Embodiment 2: Identification of Pacific bacillus YJJK-16

[0028] (1) Morphological and physiological and biochemical characteristics

[0029] The colony and thalline characteristics of the Bacillus pacificus YJJK-16 strain: cultured on LB medium at 37°C for 2 days, the colony is milky white, 1.5-2.5mm in diameter, translucent, raised, smooth and moist on the surface, with regular edges, The texture is relatively uniform; the bacteria are rod-shaped and have spores. The LB medium is the Luria-Bertani medium, and its formula is: 5 g of yeast powder, 10 g of tryptone, 10 g of sodium chloride, 20 g of agar, 1000 mL of water, and pH 7.0.

[0030] The physiological and biochemical characteristics of the Bacillus pacificus YJJK-16 strain: positive Gram staining test, positive contact enzyme test, positive starch hydrolysis test, positive motility test, positive nitrate reduction test, positive gelatin liquefaction test, D-glucose The acid production test is positive, the an...

Embodiment 3

[0034] Embodiment 3: the preparation of bacillus pacificus YJJK-16 bacterial agent

[0035] The preparation method of described bacillus pacificus YJJK-16 bacterial strain bacterial agent comprises the following steps:

[0036] 1) Activation of strains: Transfer the low-temperature-preserved Bacillus pacificus YJJK-16 strains to the slant of LB medium test tubes, and culture them at 37°C for 24 hours for activation. The LB medium is the Luria-Bertani medium, and its formula is: 5 g of yeast powder, 10 g of tryptone, 10 g of sodium chloride, 20 g of agar, 1000 mL of water, and pH 7.0.

[0037] 2) Preparation of seeds in a triangular flask: Scrape the activated lawn of Bacillus pacificus YJJK-16 with an inoculation loop, inoculate it in LB medium, and incubate at 37°C for 24 hours. The LB medium is the Luria-Bertani medium, and its formula is: 5 g of yeast powder, 10 g of tryptone, 10 g of sodium chloride, 20 g of agar, 1000 mL of water, and pH 7.0.

[0038] 3) Preparation of ...

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Abstract

The invention discloses saline-alkaline resistant Bacillus pacificus, and a biogen and application thereof. Bacillus pacificus YJJK-16 isolated from rhizosphere soil of chinese cabbages in saline andalkaline land is preserved in a general microbial center of China committee for culture collection of microorganisms on July 26, 2009, and the preservation number is CGMCC No.18300. Propyl gallate, biochemical fulvic acid powder and kathon are added into fermentation liquid of bacterial strains and stirred evenly to obtain the biogen. The Bacillus pacificus YJJK-16 has high saline-alkaline resistance, the colonization rate in saline alkali soil is high, the antagonistic effect is high on chinese cabbage soft rot pathogenic bacteria, the Bacillus pacificus YJJK-16 can be used for production ofmicrobial fertilizer, especially microbial fertilizer special for the chinese cabbages in the saline and alkaline land, is used for biological control of chinese cabbage soft rot, occurrence of diseases is reduced, and the yield and the quality are improved.

Description

technical field [0001] The invention relates to a saline-alkali-tolerant pacific bacillus and its application in preventing and treating cabbage soft rot, belonging to the field of agricultural biotechnology. Background technique [0002] Chinese cabbage, also known as yellow sprouts and Chinese cabbage, is a Brassica plant in the family Brassicaceae. It is widely cultivated in my country and is known as the "king of all vegetables". Soft rot has always been one of the key factors restricting the high-yield, high-quality cultivation and large-scale development of Chinese cabbage. It occurs commonly in all parts of the country, and the damage period is very long. It can cause cabbage to rot and cause very serious losses in the field, during storage and transportation, and even in the market. The yield loss can reach 30%-50%, and the storage loss can reach about 20%. [0003] The pathogenic bacteria of soft rot belong to the genus Erwinia (Erwinia), which are divided into thr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/07
CPCC12N1/20C12N1/205C12R2001/07
Inventor 孙中涛辛寒晓刘丽英赵升远孙国科杨越超史庆华候汉学寇娟妮刘珂欣
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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