Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for evaluating anti-aging effect of skin by combining multiple in-vitro models based on enzymology system

A model evaluation and anti-aging technology, which is applied in biochemical equipment and methods, scientific instruments, microbial measurement/inspection, etc., can solve problems such as high price, inability to realize large-scale application, irregular expression of markers, etc.

Inactive Publication Date: 2019-12-31
GUANGZHOU HUADAI BIOLOGICAL TECH CO LTD
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A few of these three-dimensional reconstructed tissue-engineered skins have been commercially available, providing a source of material for the evaluation of skin anti-aging efficacy, but they are expensive and cannot be applied on a large scale in the short term, and there are many full-thickness skin models for anti-aging effects. The cell co-culture technology is immature, the tissue structure is relatively simple, the differentiation of each cell layer is poor, and the defects such as irregular expression of markers, so further improvement is needed
[0009] The in vitro methods for evaluating the anti-aging efficacy of cosmetic raw materials and final products in the above three dimensions have certain limitations, and lack weight analysis and decision-making strategies. In-depth research should be conducted to establish hierarchical step-by-step models, or develop feasible integrated testing strategies
In addition, the mechanism of aging is very complex, and the selection and reasonable combination of evaluation endpoints are two major factors for the practicality of the solution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating anti-aging effect of skin by combining multiple in-vitro models based on enzymology system
  • Method for evaluating anti-aging effect of skin by combining multiple in-vitro models based on enzymology system
  • Method for evaluating anti-aging effect of skin by combining multiple in-vitro models based on enzymology system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Evaluation of the anti-aging effect of a certain brand of glucosyl rutin based on chemical level, cellular level, and 3D skin model

[0072] 1. Prediction of anti-aging efficacy of raw materials based on chemical level

[0073] Elastase inhibition test: 100 μL of 0.2 mol / L tris-HCl buffer (Ph=8.0), 25 μL of 10 mmol / L MAAPVN (N-methoxysuccinyl-alanine-alanine-proline Amino acid-valine-4-nitroaniline) and 50 μL of raw material dilution were mixed, and incubated at 25°C for 15 minutes; then 25 μL of 0.3U / mL elastase (the highest activity) was added to continue incubation for 15 minutes. The microplate reader detects the OD value under the condition of 410nm, and calculates the inhibition rate by the following formula. EGCG ((Epigallocatechin gallate, epigallocatechin gallate) is a positive control. Inhibition rate (%)=[(absorbance of control group-absorbance of sample group) / absorbance of control group]×100%. Different concentrations of test substances There is...

Embodiment 2

[0106] Example 2 Evaluation of anti-aging effects of a certain brand of tocopherol derivatives based on chemical level, cellular level, and 3D skin model methods

[0107] 1. Prediction of anti-aging efficacy of raw materials based on chemical level

[0108] Hyaluronidase inhibition test: Mix 100 μL of hyaluronidase, 100 μL of PBS buffer, 50 mmol / L of NaCl and 0.01% BSA with 50 μL of sample solution as a medium, and incubate in the medium at 37°C for 10 min . Then add 100 μL of hyaluronidase solution (0.03%, 300 mmol / L phosphate solution, pH=5.35) as the start of reaction, and incubate at 37° C. for 45 min. Unhydrolyzed hyaluronic acid was precipitated with 1.0 mL of acid albumin solution (0.1% BSA solution 24 mmol / L sodium acetate and 79 mmol / L acetic acid, pH=3.75). Then the mixture was left at room temperature for 10 min, and the absorbance of the mixture was detected at 600 nm with a microplate reader. The test group without enzyme was the control of the maximum inhibiti...

Embodiment 3

[0142] Example 3 Evaluation of the anti-aging effect of a certain brand of collagen derivatives based on chemical level, cellular level, and 3D skin model methods

[0143] 1. Prediction of anti-aging efficacy of raw materials based on chemical level

[0144] 1.1 Collagenase inhibition test: Use ninhydrin reaction to monitor the amount of peptides fragmented by collagenase, mix collagen I with TES (pH7.5, containing Tris-HCl, EDTA and SDS) buffer, dilute The test substance and collagen IV were mixed evenly, incubated at 37°C for 5h, and then centrifuged at 2000rpm for 5min. Collect the supernatant, mix it with ninhydrin, heat at 80°C for 10min and cool down. The solution was mixed with isopropanol at a ratio of 1:1, and centrifuged at 12000 rpm for 10 min at 4 °C. The supernatant was added to a 96-well plate, and its absorbance was detected at 600 nm. Calculate inhibition rate. Take EGCG ((Epigallocatechin gallate, epigallocatechin gallate) as positive control. Inhibition r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for evaluating the anti-aging effect of skin by combining multiple in-vitro models based on an enzymology system. The method comprises the following steps: (1) predicting the anti-aging effect based on a chemical level; (2) predicting the anti-aging effect based on a cellular level; (3) predicting the anti-aging effect based on a 3D skin model; and (4) establishinga prediction model for evaluating the anti-aging effect of skin by combining multiple in-vitro models based on an enzymology system. The method can comprehensively reflect multi-level expression andnon-animal test of a test substance in the enzymology system, can perform comprehensive anti-aging effect prediction on the test substance, is rich in data information, and can be widely applied.

Description

technical field [0001] The invention belongs to the technical field of skin anti-aging evaluation, and in particular relates to a method for evaluating skin anti-aging efficacy based on an enzymatic system combining multiple in vitro models. Background technique [0002] As a barrier between the human body and the outside world, the skin plays an important protective role in human health. Skin aging is a complex multi-factorial process. Skin aging is divided into endogenous aging and exogenous aging, and the natural aging caused by age is endogenous aging. The main manifestations are dry skin, wrinkles, sagging, and sagging. Exogenous aging is mainly caused by environmental factors, including ultraviolet radiation, air pollutants, fine particulate matter (PM2.5, P10), ozone, aerosols, smoking, and exposure to harmful chemical substances. Among them, ultraviolet radiation and PM2.5 are the main exogenous factors of skin aging, and skin aging caused by long-term exposure to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/573C12Q1/02
CPCG01N33/573G01N33/5008G01N2333/95G01N2333/96494G01N2333/966G01N2333/928
Inventor 杜娟程树军冯鉴鸿
Owner GUANGZHOU HUADAI BIOLOGICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com