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Preparation method of iron oxide nanoparticle compound, compound and high-resolution cerebral blood volume MRI imaging method

A technology of iron oxide nano-particle composites, which is applied in preparations for in vivo experiments, pharmaceutical formulations, drug delivery, etc., to achieve the effects of reducing adsorption, extending the time window, and avoiding image correction

Inactive Publication Date: 2020-01-10
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, this CBV imaging technique uses the lanthanide heavy metal Gd complex for contrast enhancement. In recent years, a large number of literatures have reported that Gd contrast agents can be selectively deposited in brain tissue, which will bring certain benefits to this CBV imaging technique. risks of

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  • Preparation method of iron oxide nanoparticle compound, compound and high-resolution cerebral blood volume MRI imaging method
  • Preparation method of iron oxide nanoparticle compound, compound and high-resolution cerebral blood volume MRI imaging method
  • Preparation method of iron oxide nanoparticle compound, compound and high-resolution cerebral blood volume MRI imaging method

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preparation example Construction

[0039] The invention provides a method for preparing iron oxide nanoparticle composites, comprising the following steps:

[0040] Polyacrylic acid-Fe 3 o 4 The preparation of polyacrylic acid is completely dissolved in polyol solvent, then iron acetylacetonate is added, in an inert gas environment, after heating to remove water, continue to heat until iron acetylacetonate thermally decomposes, and the decomposition product Fe 3 o 4 Combined with the partial carboxyl group of polyacrylic acid, after heating and refluxing, iron oxide nanoparticles modified in situ by polyacrylic acid are obtained, and the structure is polyacrylic acid-Fe 3 o 4 ;

[0041] For the second surface modification, the amino group in monoaminopolyethylene glycol is coupled with the carboxyl group of the polyacrylic acid-modified iron oxide nanoparticles prepared in step S1 to obtain iron oxide modified again with monoaminopolyethylene glycol Nanoparticle composite with the structure of monoaminopol...

Embodiment 1

[0045] Preparation of iron oxide nanoparticle composites, the process flow chart of the preparation method is as follows figure 1 as shown, figure 1 Medium Fe(acac) 3 Indicates iron acetylacetonate, PEG-NH 2 Indicates monoaminopolyethylene glycol.

[0046] (1) Polyacrylic acid-Fe 3 o 4 preparation of

[0047] Add polyacrylic acid with a molecular weight of 2000 and a monomer content of 4mmol and 25ml of triethylene glycol solvent into a 100ml L three-necked flask. After PAA (polyacrylic acid) is completely dissolved in triethylene glycol and becomes clear, add 1mmol of acetylacetone For iron, pass inert gas argon for 5 minutes; then heat to 120°C at a rate of 3°C / min, keep warm and remove water for 1h; continue to heat at a rate of 3°C / min to 250°C until iron acetylacetonate thermally decomposes, and the decomposition product Fe 3 o 4 Adsorbed by polyacrylic acid, the reaction system gradually changed from reddish brown to black solution, and iron oxide nanoparticles mo...

Embodiment 2

[0055] Preparation of Iron Oxide Nanoparticle Composite

[0056] (1) Polyacrylic acid-Fe 3 o 4 preparation of

[0057] Add polyacrylic acid with a molecular weight of 4000 and a monomer content of 1mmol and 25ml of diethylene glycol solvent into a 100ml L three-necked flask. After PAA (polyacrylic acid) is completely dissolved in triethylene glycol and becomes clear, add 1mmol of acetylacetone For iron, pass inert gas argon for 7 minutes; then heat to 100°C at a rate of 2°C / min, keep warm to remove water for 1h; continue to heat at a rate of 5°C / min to 250°C until iron acetylacetonate thermally decomposes, and the decomposition product Fe 3 o 4 Adsorbed by polyacrylic acid, the reaction system gradually changed from reddish brown to black solution, and iron oxide nanoparticles modified in situ by polyacrylic acid were prepared, with the structure of polyacrylic acid-Fe 3 o 4 ;like figure 1 As shown, the inner core is Fe 3 o 4 , by adsorbing polyacrylic acid 2 to Fe 3 ...

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Abstract

The invention provides a preparation method of an iron oxide nanoparticle compound, which comprises the following steps: carrying out in-situ modification by using polyacrylic acid in the iron oxide preparation process, and carrying out secondary surface modification by using monoamino polyethylene glycol to obtain the iron oxide nanoparticle compound subjected to twice surface modification. The invention also provides the compound and a high-resolution cerebral blood volume MRI imaging method thereof. The size of the iron oxide nanoparticle compound provided by the invention is far greater than that of a clinical gadolinium complex contrast agent, so that the probability that the iron oxide nanoparticle compound leaks from a blood vessel after being injected into the blood vessel is reduced; meanwhile, a polyethylene glycol modifier can effectively reduce adsorption of plasma protein to the iron oxide nanoparticle compound, a time window of MRI image acquisition after intravenous injection is prolonged, a spin echo or gradient echo pulse sequence with a high spatial resolution can be selected, and the spatial resolution and the image quality of the obtained CBV image are guaranteed.

Description

technical field [0001] The invention relates to the technical field of magnetic resonance perfusion imaging, in particular to a preparation method of an iron oxide nanoparticle composite, the composite, and a high-resolution cerebral blood volume MRI imaging method. Background technique [0002] Cerebral blood volume (CBV) is a very important physiological parameter to evaluate the survival and function of brain tissue. Under normal physiological conditions, there will be certain fluctuations in cerebral blood volume due to neuron activity. This CBV signal related to neuron activity is often used in functional magnetic resonance brain imaging research; on the other hand, in stroke, brain tumor And in pathological states such as Alzheimer's disease, cerebral blood volume is also closely related to the process of many nervous system diseases. Therefore, accurate measurement of cerebral blood volume, an important perfusion parameter, is of great scientific significance for fun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12
CPCA61K49/186A61K49/126
Inventor 易佩伟王慧杨晓冬
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI