Wheat protein peptide with blood pressure-lowering activity and preparation method of wheat protein peptide

Abstract

The present invention provides wheat protein peptide with blood pressure-lowering activity and a preparation method of the wheat protein peptide. The preparation method comprises the following steps:using electrolyzed water to pretreat wheat gluten meal, preparing a wheat gluten meal mixed suspension, conducting stepwise enzymolysis by using alkaline protease and protease derived from pseudomonasaeruginosa, and conducting filtration, concentration and spray-drying. The method is used to prepare the low-molecular-weight wheat peptide, a protein recovery rate is 65% or more, and the wheat peptide with a molecular weight of less than 1,000 Da accounts for 95% or more. The method is high in conversion rate of a blood pressure-lowering peptide, the in-vitro ACE semi-inhibition concentration is 0.21 mg / mL by in-vitro activity determination, and the wheat protein peptide also has good gastrointestinal digestive stability; and two novel peptides (SAGGYIW and APATPSFW) with high ACE inhibition activity are isolated and identified from the wheat peptide prepared by the method, and the in-vitro ACE half inhibitory concentrations are 2.65 [mu]M and 41.1 [mu]M, respectively.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a wheat protein peptide with blood pressure lowering activity, a preparation method thereof, and an application thereof in preparing blood pressure lowering food or medicine. Background technique [0002] Biologically active peptides generally consist of 2-20 amino acids, and are released from the protein matrix by enzymatic hydrolysis (Moller et al. European Journal of Nutrition, 2008, 47:171-182). At present, many studies have proved that bioactive peptides have low antigenic properties, are easier to absorb than parent proteins, and have various physiological regulatory activities, including antibacterial activity, antithrombotic activity, blood pressure lowering activity, antioxidant activity and immunomodulatory activity. Wait. Bioactive peptides are derived from food proteins, which have lower drug resistance induction than traditional drugs, and are considered a...

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Problems solved by technology

The ultrasonic pretreatment process will be accompanied by heat generation, which requires high technical equipment requirements and strict control of ultrasonic power, temperature and other conditions; extrusion pretreatment requires special equipment, and the pretreatment material needs to be dried in the later stage to increase the process; although high-temperature cooking The pretreatment equipment is simple, but studies have shown that long-term high-temperature pretreatment will produce lysalanine that is harmful to the human body

The above invention patents all disclose the method of raw material pretreatment combined with protease hydrolysis to prepare bioactive peptides. At present, there is no report on the use of electrolyzed water pretreatment for the preparation of bioactive peptides.

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