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Nucleic acid removing agent

A nucleic acid and reagent technology, applied in the field of molecular biology, can solve problems such as corrosion and irritation, and achieve the effects of simple and convenient operation, mild and non-irritating odor, and convenient storage.

Active Publication Date: 2020-01-14
青岛立见生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nucleic acid removal agent developed by the present invention can effectively solve the problems of corrosion, irritation, high efficiency and stability encountered in the process of nucleic acid degradation

Method used

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Examples

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Effect test

Embodiment 1

[0021] A nucleic acid remover, containing the following components and mass percentage: 5% hydrogen peroxide, 0.1% sodium hypochlorite, 0.1 oxalic acid, 0.1% acetic acid, 1% AEO-9, the rest is made up with deionized water, and the final pH value is adjusted to 3.5 .

Embodiment 2

[0023] A nucleic acid remover, containing the following components and mass percentage: 10% hydrogen peroxide, 3% sodium hypochlorite, 3% oxalic acid, 2% acetic acid, 8% AEO-9, the rest is made up with deionized water, and the final pH value is adjusted 3.5.

Embodiment 3

[0025] Utilize the nucleic acid removal agent described in embodiment 1 and embodiment 2 to degrade the genome, the specific method is as follows:

[0026] (1) DNA / RNA genome extraction kit to extract nucleic acid

[0027] ①Take 0.2 g of pig liver sample, add 5 times the weight of normal saline, grind it evenly, add 20 μL of proteinase K solution, mix well, and lyse in a water bath at 56°C for 15 minutes;

[0028] ② Centrifuge at 12,000 rpm at room temperature for 5 minutes, take 200 μL of the supernatant and add it to a new centrifuge tube, then add 200 μL of buffer solution, mix thoroughly by inversion, and place at 70°C for 10 minutes;

[0029] ③ Add 200 μL of absolute ethanol, shake and mix well for 15 seconds;

[0030] ④ Add the above solution into an adsorption column, centrifuge at 12,000 rpm for 30 seconds, discard the waste liquid, and put the adsorption column back into the collection tube;

[0031] ⑤ Add 500 μL of washing solution to the adsorption column, centrif...

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Abstract

The invention relates to a nucleic acid removing agent, which belongs to the field of molecular biology. The nucleic acid removing agent contains the following components in percentage by volume: 5%-10% of an oxidant, 0.1%-3% of a chlorine-containing reagent, 0.1%-3% of an acid-containing reagent, 0.1%-2% of a stabilizer, 1%-8% of a surfactant and the balance deionized water. The nucleic acid removing agent is odorless, is mild in properties, possesses no irritation effect, is simple and convenient to operate, is convenient to store and long in storage period, can be directly sprayed on the ground, wall surfaces and tabletops of laboratories, the tabletops of biosafety cabinets, the surfaces of pipettors, the outer surfacess of various instruments, the outer walls of centrifugal tubes andthe like during use, and can also be directly used for wiping the instruments, the tabletop, the tabletop and the like. By using the nucleic acid removing agent, nucleic acid fragments can be effectively degraded within several minutes, and laboratory aerosol pollution is prevented.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a nucleic acid removing agent. Background technique [0002] Since its inception in 1985, PCR technology has been widely used in all life-related sciences such as medicine, forensic science, agronomy, and biology, and has become one of the necessary basic technologies in the field of life research. In particular, the fluorescent PCR technology developed based on ordinary PCR technology is more than 100 times more sensitive than ordinary PCR, and is favored by a large number of scientific researchers. It has become a common method for the diagnosis of disease pathogens in the fields of medicine and veterinary medicine. But at the same time, the unique principle of PCR technology tells us that one molecule will produce billions of molecules after PCR reaction. This high sensitivity means that during the operation of PCR, the environment and laboratory are extremely slig...

Claims

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Application Information

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IPC IPC(8): C11D1/72C11D1/66C11D1/29C11D3/20C11D3/39C11D3/04C11D3/10C11D3/60C11D11/00C07H21/04C07H1/00B01D53/78B01D53/72
CPCC11D1/72C11D1/008C11D1/662C11D1/29C11D3/2079C11D3/2082C11D3/3942C11D3/046C11D3/10C07H21/04C07H1/00B01D53/78B01D53/72B01D2251/106B01D2251/108B01D2251/70B01D2257/704C11D2111/24C11D2111/14
Inventor 张志官丽娟宫枫举李翠翠孙学强
Owner 青岛立见生物科技有限公司
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