Preparation of a direct-writing 3D printing bio-ink and its 3D printing method
A 3D printing and bio-ink technology, applied in the direction of biochemical equipment and methods, microorganisms, 3D object support structures, etc., can solve the problems of easy dispersion and poor stability of fibers, and achieve high biocompatibility, adjustable viscosity, and preparation The effect of simple method
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Embodiment 1
[0042] A preparation method for direct writing molding 3D printing bio-ink, comprising the steps of:
[0043] S1. Preparation of cellulose nanofiber methacrylate freeze-dried fibers
[0044] Disperse 1-5 g of cellulose nanofiber powder in deionized water at 4-50° C., and mechanically stir for 30-120 min at a rotational speed of 200-1000 r / min to disperse evenly. Stirring in a water bath at 4-25°C for 12-24 hours to obtain a cellulose nanofiber dispersion liquid with a mass percentage of 1-5%. According to the mass of cellulose nanofiber powder in the dispersion, add 1 to 20 times the volume of methacrylic anhydride liquid dropwise at a rate of 0.5 to 1 mL / min, and react for 2 to 3 hours, then add 3 to 10 mol / L of hydroxide sodium solution, adjust the pH value of the mixed solution to the range of 8.0-9.0, and continue to react for 12-24 hours to obtain the cellulose nanofiber methacrylate precursor. Clamp the cellulose nanofiber methacrylate precursor with a 5000-14000 molec...
Embodiment 2
[0061] A direct-writing 3D printing bio-ink, the preparation process of which is the same as in Example 1, except that in step S1, 1g of cellulose nanofiber powder is added, and in the prepared cellulose nanofiber methacrylate liquid, the fiber The mass percentage of the plain nanofiber methacrylate is 1%, the mass percentage of the photoinitiator is 0.05%, the mass percentage of the PBS buffer solution is 98.95%, and the sum of the mass percentages of the above components is 100%.
[0062] The LAP photoinitiator used in the present invention has an improved polymerization reaction kinetic mechanism, so that the macromolecular hydrogel or other polymer materials can encapsulate biological cells under lower initiator concentration and longer-wavelength light. Under the action of blue light, LAP rapidly triggers the curing of photosensitive hydrogel materials. Compared with ultraviolet photoinitiators, blue light has less damage to biological cells, thereby increasing the surviv...
Embodiment 3
[0064] A kind of direct writing molding 3D printing biological ink, its preparation process is the same as embodiment 1, difference is that in step S1, add 5g cellulose nanofiber powder, in the prepared cellulose nanofiber methacrylate liquid, fiber The mass percentage of plain nanofiber methacrylate is 5%, the mass percentage of photoinitiator is 0.5%, the mass percentage of PBS buffer solution is 94.5%, and the sum of the mass percentages of the above components is 100%.
[0065] The cellulose nanofiber used in the present invention is a natural polymer material, which has the characteristics of adjustable viscosity, structural orientation, wide sources, good mechanical properties and the like. Grafting olefin groups in the cellulose nanofiber system enables it to obtain the photocurable properties of artificially synthesized polymers. A stable cellulose nanofiber methacrylate hydrogel can be prepared by photocuring induced by blue light.
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