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Autotrophic denitrification nitrogen-removal microbial inoculum and preparation method thereof

A technology of nitrifying denitrifying bacteria agent and Thiobacillus denitrifying bacteria, applied in biochemical equipment and methods, chemical instruments and methods, methods based on microorganisms, etc., can solve the problem of restricting the use of autotrophic denitrifying bacteria and the difficulty of cultivating autotrophic bacteria , long debugging time and other problems, to achieve the effect of quickly starting autotrophic denitrification, saving the time for inoculation and film formation, and fast sewage treatment time

Inactive Publication Date: 2020-02-21
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, autotrophic denitrification also has disadvantages, that is, autotrophic bacteria are difficult to cultivate and grow slowly, which makes it take a long time to debug in engineering applications.
Taking the sulfur-limestone column as an example, it takes more than 10 days to complete the inoculation and film formation, which largely restricts the application of autotrophic denitrifying bacteria in engineering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] First prepare 500ml of medium according to the following formula: manganese sulfate 0.02g / L, dibasic sodium phosphate 1.2g / L, potassium dihydrogen phosphate 1.8g / L, sodium bicarbonate 1g / L, magnesium sulfate heptahydrate 0.4g / L, Ammonium chloride 0.5g / L, calcium chloride 0.05g / L, ferric chloride 0.02g / L, potassium nitrate 5g / L, sodium thiosulfate 10g / L, the rest is water, adjust PH=7.

[0028] After preparation, sterilize at 121°C and cool to room temperature, then inoculate the slant strain of Thiobacillus denitrificans, and cultivate it at 30°C for about 15-20 days, stirring continuously during this period, the culture solution becomes milky white and turbid and produces gas, and the number of viable bacteria reaches 500 million per milliliter has been cultivated, which is the biological denitrification functional agent.

[0029] Preparation of embedding agent: Take 18g of sodium alginate, 2g of polyvinyl alcohol, add 500ml of water, heat at 90°C for 20 minutes to dis...

Embodiment 2

[0034] First prepare 500ml of medium according to the following formula: manganese sulfate 0.02g / L, dibasic sodium phosphate 1.2g / L, potassium dihydrogen phosphate 1.8g / L, sodium bicarbonate 1g / L, magnesium sulfate heptahydrate 0.4g / L, Ammonium chloride 0.5g / L, calcium chloride 0.05g / L, ferric chloride 0.02g / L, potassium nitrate 5g / L, sodium thiosulfate 10g / L, the rest is water, adjust PH=7.

[0035] After preparation, sterilize at 121°C and cool to room temperature, then inoculate the slant strain of Thiobacillus denitrificans, and culture at 30°C for about 15-20 days, stirring continuously during this period, the culture solution appears milky white and turbid and produces gas, and the number of viable bacteria detected reaches 500 million per milliliter has been cultivated, which is the biological denitrification functional agent.

[0036] Preparation of embedding agent: Take 20g of sodium alginate, add 500ml of water, heat at 90°C for 20 minutes to dissolve completely, coo...

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Abstract

The invention discloses an autotrophic denitrification nitrogen-removal microbial inoculum and a preparation method thereof, which relate to the technical field of sewage treatment. The autotrophic denitrification nitrogen-removal microbial inoculum comprises a biological nitrogen-removal functional agent, an embedding agent, a cross-linking agent, a nutritional agent and an environmental solvent,the thiobacillus denitrificans in an optimal active state and required nutrients are prepared into an active microbial agent by utilizing an immobilized cell technology, and the active microbial agent can be directly used, so that the purposes of realizing immediate use after being put into use, greatly shortening the debugging time of an autotrophic denitrification process and greatly simplifying the operation process are achieved. The invention further comprises a preparation method of the autotrophic denitrification nitrogen-removal microbial inoculum, so that the active microbial agent with the best activity and the maximum bacteria amount is obtained and used for embedding, and the purpose that the effect of the autotrophic denitrification nitrogen-removal microbial inoculum is better is achieved.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a bacterial agent for sewage biological treatment and a preparation method thereof. Background technique [0002] Nitrogen is a major source of pollution in sewage, which mainly exists in the form of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and organic nitrogen, which is very harmful to water pollution, poisoning aquatic organisms (ammonia nitrogen), causing eutrophication Lack of oxygen can even worsen water bodies. The country has very strict controls on nitrogen in wastewater, so the removal of nitrogen in sewage treatment is an important process. [0003] At present, the removal methods of nitrogen in sewage are divided into physical and chemical methods and biological methods. The physical and chemical methods mainly use technologies such as ion exchange, membrane separation, and stripping. These methods generally have disadvantages such as high investm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/084C12N11/04C02F3/34C02F101/16C12R1/01
CPCC02F3/34C02F2101/163C02F2305/06C12N1/20C12N11/04C12N11/08C12N11/10
Inventor 周斌肖炜吴少华
Owner YUNNAN UNIV