Method for extracting ganoderma triterpenes by continuous phase transition and ganoderma triterpenes

Abstract

The invention discloses a method for extracting ganoderma triterpenes by continuous phase transition and the ganoderma triterpenes. The method for extracting the ganoderma triterpenes by continuous phase transition includes the steps: slicing, crushing and then drying blocky lucid ganoderma until water content is lower than or equal to 15% to obtain lucid ganoderma powder, and extracting polysaccharides by continuous phase transition to obtain lucid ganoderma residues; taking ethanol water solution as an extraction agent after drying, enabling the extraction agent to flow through an extractionkettle at the flow speed of 20-100L / h under the conditions of the extraction temperature of 60-90 DEG C, the pressure of 0.02-0.25MPa and the ethanol concentration of 50%-95%, and performing continuous extraction for 120-300 minutes. Analysis temperature is 50-75 DEG C, and analysis pressure ranges from -0.10MPa to 0.10MPa. Compared with a traditional Soxhlet extraction method, the method has theadvantages that the extraction rate of the ganoderma triterpenes is increased by 10%-30%, extraction time is shortened by 40%-60%, the method is high in extraction rate, strong in operability, environmentally friendly and suitable for industrial production, and energy is saved.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation. More specifically, it relates to a method for continuous phase-change extraction of ganoderma triterpenes and ganoderma triterpenes. Background technique [0002] Ganoderma lucidum is a kind of large fungus, which belongs to Basidiomycetes, Polyporaceae, Polyporaceae, and Ganoderma genus in fungal classification. There are many active components in Ganoderma lucidum and their composition is complex. Currently, more than 150 compounds have been isolated, mainly including Polysaccharides, triterpenoids, sterols, alkaloids, furan derivatives, aminopeptides, and inorganic elements. Among them, Ganoderma lucidum triterpenoids are one of the important chemical components of Ganoderma lucidum, which have anti-tumor, immune regulation, hypoglycemic, anti-aging, anti-oxidation and scavenging free radicals effects. The biggest feature of Ganoderma lucidum triterpenes is that it can acti...

AI Technical Summary

Problems solved by technology

Traditional heat extraction and Soxhlet extraction have low extraction rate and long time, and higher temperature will destroy heat-sensitive active substances in Ganoderma lucidum, etc.

Ultrasonic extraction, microwave extraction and supercritical extraction have small processing capacity, high cost, complicated process, and high industrial application cost

[0005] Most of the evaluation methods disclosed in the existing documents or patents on the extraction process of triterpenoids are analyzed by HPLC, and the standard substance position of known molecular weight is used to judge whether the extract exists or to calibrate the distribution interval of the molecular weight. This method is used in biological In the field of chemistry, it is a conventional method, which is rarely used in the extraction process of traditional Chinese medicine due to its complexity, and because the method uses high performance liquid phase method, the injection volume is relatively small, resulting in relatively low detection sensitivity

The evaluation method of "single lower limit" for the quantification of individual "active ingredients (or index ingredients)" that is currently widely used completely draws on the research ideas of natural medicines and is not suitable for the evaluation of the extraction process of traditional Chinese medicines.

[0006] Patent No. CN1560072A discloses a method for preparing Ganoderma triterpenes and Ganoderma polysaccharides from Ganoderma lucidum. After the dried Ganoderma lucidum is crushed, supercritical CO 2 Extraction technology extracts Ganoderma lucidum triterpenoids, and then uses low-temperature extraction and rapid concentration technology to obtain Ganoderma lucidum polysaccharides, but adopts supercritical CO 2 The equipment required for the extraction technology is relatively large, the operation is difficult, and the production cost is high

Patent No. CN105294875B discloses a method for extracting Ganoderma lucidum polysaccharides from Ganoderma lucidum fruiting bodies. After the Ganoderma lucidum fruiting bodies are pulverized, the cellulose and lignin that make up the cell wall are reacted with the oxidation system of sodium chlorite-glacial acetic acid. Digestion and fracture release more active substances, which greatly improves the extraction efficiency of polysaccharides. However, Ganoderma lucidum triterpenoids and polysaccharides are mostly used in medicine and health care, and sodium chlorite is highly carcinogenic. Extracting products with this method is likely to cause safety hazards

The patent publication No. CN 109893547 A discloses a method for extracting ganoderma triterpene acids and ganoderma polysaccharides from ganoderma lucidum, which is pretreated by using sodium hydroxide-water solution and extracted by means of dynamic reflux adsorption, and the process is complicated. High cost, not suitable for industrial mass production

Images