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Fluorine-modified silicon rubber with protein non-specific adsorption resistance, preparation method and applications thereof

A non-specific, silicone rubber technology, applied in the field of surface modification of biomedical materials, can solve the problems of electrode conductivity isolation, loss of effectiveness, protein adhesion, etc., to achieve increased thickness, long action time, and good biocompatibility Effect

Pending Publication Date: 2020-04-07
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term implantation of silicone electrodes will cause protein adhesion and cell adhesion, resulting in the isolation of the conductivity of the electrode and loss of effectiveness

Method used

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  • Fluorine-modified silicon rubber with protein non-specific adsorption resistance, preparation method and applications thereof
  • Fluorine-modified silicon rubber with protein non-specific adsorption resistance, preparation method and applications thereof
  • Fluorine-modified silicon rubber with protein non-specific adsorption resistance, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]Put a 10cm×10cm×1mm silicone rubber sheet in a 1L beaker and ultrasonically clean it with deionized water, then dry it at a vacuum of 10KPa and a temperature of 129°C for 24h, and clean the surface of the silicone rubber under 110W UV light for 20 minutes , to remove surface oxides and other contaminants. Immediately after ozone treatment, immerse the silicone rubber sheet in deionized water and incubate in an oven at 80°C for 24 hours, then dry it in nitrogen, vacuum at 50KPa, and temperature at 25°C for 24h, and store it in a sealed container; at room temperature, it is calculated by volume percentage 5% Tridecafluorooctyltriethoxysilane (PFOTES) and 81% methanol, 14% mass fraction of 36% acetic acid aqueous solution are added to a dry petri dish, the total volume of the mixed solution is 20ml; Clean the silicon rubber sheet (10cm×10cm×1mm), seal the petri dish with plastic wrap, put it in a vacuum drying oven, vacuumize it for about 15min (vacuum degree is 50KPa), and...

Embodiment 2

[0050] The silicone rubber modified with tridecafluorooctyltriethoxysilane obtained in Example 1 was washed 3 times with deionized water, soaked in PBS buffer solution (pH=7.4) for 60 min, N 2 Blow dry; Petri dish is rinsed 3 times with PBS damping fluid (pH=7.4); Bovine serum albumin is mixed with the PBS buffering fluid of pH=7.4 to be the BSA solution that concentration is 5mg / L; Then BSA solution is added into petri dish, Add tridecafluorooctyltriethoxysilane-modified silicone rubber (completely submerged in BSA solution), soak at room temperature for 24 hours, take out the silicone rubber after the reaction, wash it with PBS buffer solution for 3 times, and then wash it with deionized water for 3 times. then use N 2 Blow dry, and finally observe the fluorescence under a fluorescence microscope.

Embodiment 3

[0052] Rinse the unmodified silicone rubber and PFOTES-modified silicone rubber in Example 1 once with appropriate amount of distilled water, blow dry with nitrogen, and incubate in 20mL of 37°C physiological saline for 0.5h, then modify the silicone rubber and PFOTES Place the silicone rubber in an ultra-clean bench for ultraviolet sterilization, and then add it to a 24-well cell culture plate (the size of the silicone rubber or modified silicone rubber in each well is 0.5cm×0.5cm×1mm), and add 500μL DMEM to each well Complete medium (DMEM basal culture plus 5% fetal bovine serum (brand Gibco, product number A3160802) and 1% double antibody (brand Gibco, product number 15140-122, wherein double antibody components: each milliliter double antibody contains 10000units penicillin and 10000μg streptomycin) in 5% CO 2 Equilibrate in a 37°C incubator for 24 hours. Aspirate the medium, add 500 μL of mouse fibroblast (L929) cell suspension (the medium is DMEM complete medium), in wh...

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Abstract

The invention discloses a fluorine-modified silicon rubber with protein non-specific adsorption resistance, a preparation method and applications thereof. The method comprises the following steps: (1)soaking a silicon rubber into water, and incubating to obtain a treated silicon rubber; and (2) uniformly mixing 1-10% by volume of fluorine-containing silane, 78-81% by volume of methanol and 12-18%by volume of an acetic acid aqueous solution, adding the silicon rubber treated in the step (1), reacting, washing, and drying to obtain the fluorine-modified silicon rubber with protein non-specificadsorption resistance. According to the invention, a silicon rubber is modified with fluorine-containing silane with hydrophobic property to construct a silicon rubber surface with protein adhesion resistance and fibroblast adhesion resistance, and the properties of the material are verified through cell experiments, so that the protein adhesion resistance and the fibroblast resistance of the silicon rubber surface are enhanced; and the obtained material has good protein adhesion resistance and biocompatibility, and is a modified material capable of being used in the field of human body implantation equipment.

Description

technical field [0001] The invention belongs to the field of surface modification of biomedical materials, and in particular relates to fluorine-modified silicone rubber with anti-protein non-specific adsorption and its preparation method and application. Background technique [0002] Exposure of synthetic biomaterials to biological media often triggers a series of complex chain reactions. These interface-related phenomena often lead to some serious clinical complications, such as implant-related infection or thrombosis of blood contacting the artificial surface. It is generally accepted that the first step in this biological sequence is the interaction of the protein with the outer surface. Therefore, inhibiting the adsorption of proteins to surfaces is a key requirement for improving the biocompatibility of various biomedical devices over time. Obviously, the focus of biomaterials research is to design new materials that can resist the non-specific adsorption of proteins...

Claims

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Application Information

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IPC IPC(8): C08J7/12C08L83/04A61L31/06A61L31/14
CPCC08J7/12A61L31/06A61L31/14C08J2383/04C08L83/04
Inventor 马栋梁景兰薛巍
Owner JINAN UNIVERSITY
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