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Silk-screen printing carbon nanotube sensor and preparation method thereof and pesticide detection application

A technology of screen printing and carbon nanotubes, which is applied in the fields of analytical chemistry and electrochemical sensing, can solve the problems of cumbersome activation process, poor parallelism of electrode batches, and expensive equipment, and achieves strong anti-interference and good reproducibility. , prepare a simple effect

Pending Publication Date: 2020-05-01
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional detection of organophosphorus pesticides mainly uses chromatography-mass spectrometry technology. Although these methods can achieve accurate analysis, they are expensive in equipment, high in detection costs, poor in portability, long in sample pretreatment time and cumbersome process, and require specialized technicians to operate. Online detection cannot be realized, which also limits the demand for real-time, fast, and field detection methods pursued in analysis work
Although traditional solid-state carbon electrodes and glassy carbon electrode sensors can be used for the detection of organophosphorus pesticides, the parallelism between electrode batches is poor, and the electrode processing and activation processes are cumbersome, resulting in large deviations in the detection of electrode sensors.

Method used

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  • Silk-screen printing carbon nanotube sensor and preparation method thereof and pesticide detection application
  • Silk-screen printing carbon nanotube sensor and preparation method thereof and pesticide detection application
  • Silk-screen printing carbon nanotube sensor and preparation method thereof and pesticide detection application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Take 10 μL of 2 mg / mL PDDA solution and drop it on the interface of the activated screen-printed carbon nanoelectrode (referred to as the bare electrode), incubate for 30 min, wash with ultrapure water, and then blow dry with nitrogen to obtain the PDDA / screen-printed carbon nanotube sensor (C / PDDA), its surface covers the first PDDA modified film;

[0088] Take 5 μL of 0.1 mg / ml acetylcholinesterase solution and drop it on the interface of the above PDDA / screen-printed carbon nanoelectrode (C / PDDA), incubate for 60 min, rinse with PB buffer solution, and then blow dry with nitrogen to obtain acetylcholinesterase / PDDA / screen printing carbon nanotube sensor (C / PDDA / AChE), its outermost layer covers the first acetylcholinesterase modified membrane;

[0089] Take 10 μL of 2 mg / mL PDDA solution and drop it on the interface of the acetylcholinesterase / PDDA / screen-printed carbon nanotube sensor (C / PDDA / AChE) prepared above, incubate for 30 min, wash with ultrapure water, a...

Embodiment 2

[0096] Prepare the catalytic substrate acetylthiocholine chloride PB solution of 0.5mmol / L, 1mmol / L, 2mmol / L, 4mmol / L, 8mmol / L with PB solution (pH 7.4, 100mmol / L), the embodiment 1 The prepared PDDA / acetylcholinesterase / PDDA / acetylcholinesterase / PDDA / screen-printed carbon nanotube sensors (PDDA / AChE / PDDA / AChE / PDDA / CNTSPE) were mixed with different concentrations of acetylthiocholine chloride PB solution After acting for 20 minutes, electrochemical voltammetry (CV) measurement was carried out in the potential window of -0.2~+1.2V. The measurement results are as follows: figure 2 shown.

[0097] from figure 2 It can be seen from the figure that the CV scanning curve of the PB solution is flat and the background signal is very low; when the above sensors are respectively reacted with 0.5mmol / L and 1mmol / L acetylthiocholine chloride PB solution, the CV scanning curve has no obvious signal Change; continue to increase the concentration of acetylthiocholine chloride, that is, a...

Embodiment 3

[0099] Prepare methanol stock solution of 100mg / L pesticide chlorpyrifos (chlorpyrifos) and paraoxon (Paraoxon) standard substance respectively, prepare concentration respectively with PB (pH 7.4, 100mmol / L) buffer solution and be 50,100,200, 400, 500, 600ng / mL pesticide standard working solution.

[0100] The PDDA / acetylcholinesterase / PDDA / acetylcholinesterase / PDDA / screen printing carbon nanotube sensor (PDDA / AChE / PDDA / AChE / PDDA / CNTSPE) prepared in Example 1 was mixed with different concentrations of chlorpyrifos and paraoxon respectively. The solution was reacted for 20 minutes, after taking it out, it was rinsed with PB buffer solution, blown dry with nitrogen, and then incubated with PB solution of 4mol / L acetylthiocholine chloride for 30 minutes, and the electrochemical signal was measured at the potential window of -0.2~+1.2V. The linear relationship between electrochemical response signal and pesticide concentration is as follows: image 3 shown.

[0101] from image...

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Abstract

The invention relates to a silk-screen printing carbon nanotube sensor and a preparation method thereof and pesticide detection application. The silk-screen printing carbon nanotube sensor comprises asilk-screen printing carbon nanotube electrode, and a first PDDA modification film, a first acetylcholinesterase modification film, a second PDDA modification film, a second acetylcholinesterase modification film and a third PDDA modification film which are sequentially coated on the surface of the silk-screen printing carbon nanotube electrode. The silk-screen printing carbon nanotube sensor canimprove the detection sensitivity and interference resistance, has the advantage of good detection reproducibility, and can be used for determining organophosphorus pesticides in an environment watersample system.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and electrochemical sensing, and relates to a method for preparing and detecting organophosphorus pesticides based on a multi-layer cholinesterase screen-printed carbon nanotube electrode sensor. Phosphorus pesticide detection. Background technique [0002] Organophosphorus pesticides will cause damage to the human immune system, nervous system, cardiovascular and cerebrovascular system, respiratory system, etc. after entering the human body through dietary routes, and also cause serious harm to human health, resulting in acute poisoning accidents and even death of a large number of individuals . [0003] The traditional detection of organophosphorus pesticides mainly uses chromatography-mass spectrometry technology. Although these methods can achieve accurate analysis, the equipment is expensive, the detection cost is high, the portability is poor, the sample pretreatment time is lo...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30B82Y15/00B82Y30/00B82Y40/00
CPCG01N27/48G01N27/308B82Y15/00B82Y30/00B82Y40/00
Inventor 梁刚潘立刚靳欣欣毕延娣徐笠
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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