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A kind of Bacillus Velez hmb28023 and its application

A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of no discovery and control of botrytis cinerea

Active Publication Date: 2021-09-14
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bacillus velezensis belongs to a new species of Bacillus, and it has been used to control wheat sheath blight, wheat head blight, rape sclerotinia, pepper root rot, bean wilt, tobacco red spot , banana fusarium wilt, rice blast and other dozens of diseases, but there is no report on the prevention and treatment of botrytis cinerea

Method used

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  • A kind of Bacillus Velez hmb28023 and its application
  • A kind of Bacillus Velez hmb28023 and its application
  • A kind of Bacillus Velez hmb28023 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Isolation procedure of Example 1 HMB28023 strain

[0030] January 16, 2013 to 20, the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry from the Tibet Autonomous Region Nyingchi Mainling, Gongbo'gyamda, Gyantse County, Xigaze, Tingri County, Jen cloth County, Lazi County, Nan Shan waves Kazi County, Lhasa Qushui, maizhokunggar county nine counties collect the 22 glacier lowlands, grasslands, Lake, Canyon, Yamaguchi and other various types of soil samples, were brought back to the laboratory weigh 1g of soil samples into 250mL sterilized flask, sterile water was added 100mL, placed in a shaker, 170 rpm shaking 30min, allowed to stand for 2h, and the supernatant was added 10mL 50mL sterile centrifuge tube, a thermostat at 80 deg.] C water bath for 30 minutes and then add 1mL take sterile water 9mL, Serve 10mL10 -3 Microbial times suspensions, soil suspension was then diluted to 10 -4 10 -5 10 -6 Fold dilution, the concentration of the microorganism susp...

Embodiment 2

[0031] Example 2 HMB28023 Identification Strain embodiment:

[0032] (1) Morphological characterization

[0033]Cells cultured on LB medium is a rod, 10H After culturing spores, spores of Health, oval, cysts not inflated, negative acid-fast staining, cell unaccompanied crystal, movable, Mao Zhousheng whip. On nutrient agar plates, cultured initial creamy white colonies, pus-like, rounded, neat edge, the colony was raised bread-like, moist surface; culture late as a pale yellow colonies, irregular edges, drying wrinkled surface; on nutrient agar slant streaked, rectilinear; stationary culture in a liquid medium, a white surface pellicle. These morphological features and "common bacterial identification system Manual" (East Xiuzhu, eds. Science Press, 2001) described the genus Bacillus morphological characteristics are basically the same, the initial judge HMB28023 strains belonging to Bacillus.

[0034] (2) using the 16S rDNA sequence identification and classification

[0035] Geno...

Embodiment 3

[0046] Preparation Example 3 HMB28023 embodiment microbial agents

[0047] Proceed as follows:

[0048] (1) strain activation: -80 ℃ to save at the Velez strain of Bacillus HMB28023 (this strain HMB28023 been in November 25, 2019 deposited at the Chinese Culture Collection Committee General Microbiology Center, under the accession number CGMCC No.19002) in LB plate medium (which components and their weight ratio: tryptone 1g, yeast extract 0.5g, NaCl 1g, agar 15g, activated (30 ℃) on a water 1000mL), pick single colony in LB slant medium (and its composition by weight is: yeast extract, 0.5g, NaCl 1g, agar 15g, water 1000mL tryptone 1g) cultured at 30 ℃ 30 hours to give activated strain;

[0049] (2) Preparation of seed solution: prepared by conventional methods LB liquid medium (and its composition by weight is: tryptone 1g, yeast extract 0.5g, NaCl 1g, water 1000mL), in a 250mL Erlenmeyer flask LB broth was charged 100mL, autoclaved, after the temperature dropped to room tempera...

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Abstract

The invention belongs to the field of biological control, and specifically discloses a Bacillus velezensis strain HMB28023, the preservation number of which is CGMCC No.19002; the invention also discloses a microbial inoculum containing the strain. The HMB28023 The control effect of the strain on Botrytis cinerea is more than 84.39%; the control spectrum is wide, the strain not only has high control effect on Botrytis cinerea, but also is effective against Pine blight, apple rot, poplar rot, walnut ulcer Bacteria and walnut brown spot bacteria also have good control effects; HMB28023 strain is highly specific to Botrytis cinerea, and is not easy to produce drug resistance; the microbial inoculum of the invention is safe for humans and animals, and has no environmental pollution problem.

Description

Technical field [0001] The present invention belongs to the field of biological control, in particular to a Velez, Bacillus bacteria also relates to the production of microbial agents, and their use. Background technique [0002] Grape diseases caused by fungi, Botrytis cinerea (Bortrytis cinerea) caused by a plurality of portions inflorescences, corolla, stems and leaves of young fruits and grapes may be the disease, thus causing annual losses grape 20% ~ 30%, 50% or more serious, therefore, grapes gray mold damage to grape production is very serious. [0003] Prevention and treatment of grape gray mold mainly uses chemical control methods, cultural practices and biological control. Chemical Control Although prevention is better, but there are environmental pollution and prone to drug resistance and other issues. The use of poor cultivation practices prevention control effect. Because of the high control effect biocontrol, strong specificity, good lasting effect, environmentally...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/689C12Q1/04A01N63/22A01P3/00C12R1/07
CPCC12Q1/689C12N1/205C12R2001/07
Inventor 王培培王雪美李社增鹿秀云马平郭庆港张晓云曲远航苏振贺赵卫松董丽红班一云
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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