Growth factor-loaded porous microsphere, and preparation method and application thereof

A technology of growth factors and porous microspheres, applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve problems such as reduced use efficiency, short residence time, and poor stability, and achieve Improved anti-inflammatory effect, high wound healing rate, and good healing effect

Pending Publication Date: 2020-05-15
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although KGF-2 has a good effect in promoting skin wound healing, its poor stability and short half-life limit its application effect, and most of KGF-2 is used in the form of solution, and the residence time is short when it is used locally on the wound , which greatly reduces the efficiency of its use
[0008] In addition, other traditional growth factors, such as epidermal growth factor (EGF), transforming growth factor (TGF), insulin-like growth factor (IGF), nerve growth factor (NGF) and platelet-derived growth factor (PDGF), are also commonly used. Poor stability and short half-life

Method used

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  • Growth factor-loaded porous microsphere, and preparation method and application thereof
  • Growth factor-loaded porous microsphere, and preparation method and application thereof
  • Growth factor-loaded porous microsphere, and preparation method and application thereof

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Embodiment

[0041] Material

[0042] Carboxymethyl chitosan (CMC, Mn = 150-200kDa, carboxylation degree ≥ 80%) was purchased from Shanghai Yuanye Biotechnology Co., Ltd., sodium alginate, Span 80, Dulbecco's modified eagle medium (DMEM), phosphate Buffer (PBS) and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Calcium chloride (purity 99.9%) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd., and liquid paraffin, n-hexane, and absolute ethanol were from Wenzhou Lucheng Jinshan Chemical Reagent Instrument Company. Collagen type III N-terminal antibody, 1:250; interleukin-6 (IL-6) antibody, 1:100; alpha smooth muscle actin (α-SMA) specific antibody, 1:250; proliferating cell nuclear antigen ( PCNA), 1:250; both were purchased from Proteintech Biotech Co., Ltd. Keratinocyte growth factor 2 (KGF-2) was provided by School of Pharmacy, Wenzhou Medical University. Ultrapure wate...

experiment example

[0053] In order to fully verify the morphology, chemical structure, sustained-release performance, and functions in hemostasis and wound healing of the porous microspheres (KGF-2-PHM) loaded with keratinocyte growth factor 2 prepared by the present application, the invention Experimental tests were carried out by human beings, as follows:

[0054] 1. Morphology and chemical structure test

[0055] The surface morphology of keratinocyte growth factor 2-loaded porous microspheres (KGF-2-PHM) was characterized by field-emission scanning electron microscopy (SEM) (Hitachi SU8010, Japan), and all samples were gold-plated at 10 mA before electron microscopy 60s, the obtained SEM images were analyzed with Nano Measurer software. The microspheres were uniformly embedded in the cured epoxy resin spline, and then the spline was broken to obtain the truncated surface of the microsphere, and then the internal structure of the microsphere was observed by SEM.

[0056] The chemical struct...

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Abstract

The invention relates to a growth factor-loaded porous microsphere, and a preparation method and application thereof, and belongs to the technical field of composite biological materials. The growth factor-loaded porous microsphere is characterized in that a used microsphere is a polymer-based porous microsphere and has good hydrophilicity and water absorption performance, and the diameter is 10 to 1000 [mu]m; shallow pits with the diameter of 0.5 to 5 [mu]m and the depth of 100 to 500 nm are formed in the surface of the microsphere; and mutually communicated micropore passages with the pore diameter of 0.05 to 1.5 [mu]m are formed at the inner walls of the shallow pits and the inside of the microsphere. The micropore passages and the shallow pits of the microsphere are fully filled with adsorbed growth factor particles. The preparation method comprises the steps that growth factors are prepared into a water solution with the concentration of 10 to 500 [mu]g / mL; the microsphere is immersed into the growth factor solution to be fully swelled; and then, the absorption swelling microsphere adsorbing the growth factor solution is subjected to freeze drying. The built growth factor-loaded porous microsphere has the advantages that by utilizing the capillary micropore passages and the unique morphology of the microsphere, the growth factors are loaded in the microsphere; the biocompatibility is good; an excellent slow-release function is realized; and the skin wound healing can be effectively promoted.

Description

technical field [0001] The invention relates to the technical field of composite biomaterials, in particular to a polymer porous microsphere loaded with growth factors and its preparation method and application. Background technique [0002] Wounds that are difficult to heal also often bring great pain to patients and even cause death. Rapid hemostasis and healing of wounds can greatly reduce the pain of patients. [0003] Growth factors are active proteins or polypeptides that have extensive regulatory effects on cell growth, proliferation, differentiation and other cell functions in organisms. Growth factors have a good promoting effect on wound healing. However, its application effect is limited due to its poor stability, short active period, and high price, and most of the growth factors are used in the form of solution, which has a short residence time when used locally on the wound, which greatly reduces its application efficiency. [0004] The porous microsphere mat...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K9/16A61K47/36A61P17/02A61P7/04
CPCA61K38/18A61K38/1808A61K38/1841A61K38/185A61K38/1858A61K38/1825A61K9/1652A61P17/02A61P7/04Y02A50/30
Inventor 石长灿席光辉李校堃王晓杰
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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