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Preparation method of injectable hydrogel precursor solution, injectable hydrogel and application of injectable hydrogel

A technology of hydrogel and precursor liquid, which is applied in the field of biomedicine, can solve the problem that the shape cannot meet people's needs, and achieve the effects of low drug delivery efficiency, good injectability, and high drug delivery efficiency

Active Publication Date: 2020-05-19
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects that the hydrogels in the prior art need surgical implantation and the shape cannot meet people's needs, thereby providing a kind of injectable hydrogel with good biocompatibility. Hydrogels have the advantages of in situ molding in vivo, without surgery, and can be molded into arbitrary shapes

Method used

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  • Preparation method of injectable hydrogel precursor solution, injectable hydrogel and application of injectable hydrogel
  • Preparation method of injectable hydrogel precursor solution, injectable hydrogel and application of injectable hydrogel
  • Preparation method of injectable hydrogel precursor solution, injectable hydrogel and application of injectable hydrogel

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Embodiment 1

[0043] The preparation method of the injectable hydrogel precursor of the present embodiment comprises the following steps:

[0044] S1. Weigh 5g of PEG2000 and 1.08g of cyanoacetic acid, dissolve them in 80mL of benzene, then add 2-3 drops of concentrated sulfuric acid, and reflux at 100°C for 24 hours to obtain a light yellow liquid; After washing with sodium bicarbonate solution and drying with anhydrous magnesium sulfate, filter; the obtained filtrate was concentrated to obtain PEG2000 cyanoacetate with a yield of 80%. In this example, saturated sodium bicarbonate solution was used to wash with water three times to remove the reaction residue in the sample.

[0045] S2. Weigh 5g of vanillin in a flask, add 30mL of anhydrous dichloromethane, then add 4.94g of triethylamine, and stir the reaction in an ice bath; add 30mL of anhydrous dichloromethane into a constant pressure separatory funnel Methane, and then add 4.60g of acryloyl chloride, adjust the piston of the constant...

Embodiment 2

[0059] Same as Example 1, the difference is that the concentrations of the first solution and the second solution in step S4 are 20%. Dissolve PEG2000 cyanoacetate and water-soluble polyaldehyde respectively in PBS aqueous solution with pH=7.4 to obtain a first solution and a second solution with a concentration of 20%; quickly mix the first solution and the second solution evenly, and let stand , to produce a hydrogel.

Embodiment 3

[0061] Same as Example 1, the difference is that the concentrations of the first solution and the second solution in step S4 are 10%. Dissolve PEG2000 cyanoacetate and water-soluble polyaldehyde respectively in PBS aqueous solution with pH=7.4 to obtain a first solution and a second solution with a concentration of 10%; quickly mix the first solution and the second solution evenly, and let stand , to produce a hydrogel.

[0062] This application is based on the hydrogel formed by reversible dynamic covalent bonds. The active methylene group provided by PEG2000 cyanoacetate and the aldehyde group provided by water-soluble polyaldehyde are catalyzed by PBS and formed by Knoevenagel reaction. , which is also the first study to extend the Knoevenagel reaction to the preparation of hydrogel systems. And CN110522948A is a kind of hydrogel system based on hydrazide bond.

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Abstract

The invention provides a preparation method of an injectable hydrogel precursor solution, an injectable hydrogel prepared by the preparation method and a use method of the injectable hydrogel. According to the preparation method, PEG2000 cyanoacetate and water-soluble polyaldehyde are used as raw materials, active methylene groups provided by the PEG2000 cyanoacetate and formyl groups provided bythe water-soluble polyaldehyde form the injectable hydrogel in situ through a Knoevenagel reaction under the catalysis of PBS. The injectable hydrogel has excellent biocompatibility and biodegradability and can be used as a carrier material for 3D cell culture.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing an injectable hydrogel precursor, the prepared injectable hydrogel, and a method for using the injectable hydrogel. Background technique [0002] Hydrogels are a class of cross-linked polymers with a three-dimensional network structure, which have a large number of hydrophilic groups or domains. These networks have a high affinity for water but are insoluble due to the presence of chemical or physical bonds formed between polymer chains. Hydrogels are cross-linked polymer networks that not only absorb and retain large amounts of water, but also mimic biological fluids in the natural extracellular matrix. Hydrogels derived from natural polymers mimic many features of the extracellular matrix, thus potentially guiding cell migration and growth during tissue regeneration and wound healing, and have the potential to stably encapsulate and transplant cells. Over the...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L71/02C08L33/24C08F220/54C08F220/30C08G65/333A61L27/52A61L27/26A61L27/50
CPCC08J3/075C08F220/54C08G65/33368A61L27/52A61L27/26A61L27/50C08J2371/02C08J2333/24A61L2400/06C08L71/02C08L33/24
Inventor 于冰丛海林焦彩彩申有青高利龙
Owner QINGDAO UNIV
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