Method for Biosynthesizing Organic Selenium Using Seafood Mushroom

A technology for seafood mushrooms and organic selenium, applied in the field of microbiology, can solve problems such as no MeSeCys, and achieve the effects of controllable amount of selenium added, short fruiting and growth time, and white and tender color

Active Publication Date: 2021-05-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported MeSeCys are mainly through chemical synthesis, see CN200710051362.3 and CN200910115585.0, etc., but no biosynthetic method is used to obtain MeSeCys

Method used

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  • Method for Biosynthesizing Organic Selenium Using Seafood Mushroom
  • Method for Biosynthesizing Organic Selenium Using Seafood Mushroom
  • Method for Biosynthesizing Organic Selenium Using Seafood Mushroom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 strain breeding and the acquisition of selenium-enriched carrier

[0033] The seafood mushroom strain H023 preserved in the Edible Mushroom Research Office of China Agricultural University was cultured on PDA medium for 14 days, and 5 mm of bacteria cakes were taken from the growth edge of the colony to inoculate sodium selenate and sodium selenite at 1.0 mg / kg respectively. , on 5.0mg / kg and 20.0mg / kg plates, cultured at 21°C for 10 days, and measured the diameter of the colony. The results showed that the colony growth of H023 was significantly inhibited under the treatment of 5.0mg / kg sodium selenite; at 25.0mg / kg Sodium selenate treatment significantly inhibited the growth of H023 mycelia ( figure 2 ).

[0034] Cultivate the seafood mushroom strain H023 preserved in the Edible Mushroom Research Office of China Agricultural University on PDA medium for 14 days, take 5mm fungus cakes from the growth edge of the colony and inoculate them on 10 PDA plates...

Embodiment 2

[0036] Embodiment 2 sodium selenate treatment selenium-enriched cultivation

[0037] The weight ratio of Douglas fir sawdust, corn cobs, soybean hulls and wheat bran with uniform particle size is 40:30:15:15, the pH is adjusted to 8 with lime powder, the moisture content is 80%, and the selenate concentration gradient is set to 0.5, 1.0, 2.5, 5.0, 10.0, 20.0, 40.0mg / kg, each culture bag is loaded with 2.0kg of mixed material, 121 ℃ high temperature, autoclave for 2 hours. After cooling to room temperature, the bacteria were inoculated. Take a 5mm uniform bacterium cake from the test tube for preserving the strain and inoculate it into a 500mL Erlenmeyer shaker flask filled with 150mL liquid PDA medium, and culture it with shaking at 170-180r / min at 21-23°C for 4 days as the first-grade bacteria kind. Use a sterile pipette to draw 5mL of bacteria from the first-level strain shake flask covered with mycelium balls and transfer it to a 500mL shake flask containing 150mL of PDA ...

Embodiment 3

[0038] Embodiment 3 Sodium Selenite Treatment Selenium-enriched Cultivation

[0039] Use Douglas fir sawdust, corn cobs, soybean hulls, and wheat bran with a weight ratio of 40:30:15:15, use lime powder to adjust the pH to 8, the moisture content is 80%, and the selenite concentration gradient is set 0.5, 1.0, 2.5, 5.0, 10.0, 20.0, 40.0 mg / kg, each culture bag is filled with 2.0 kg of mixed material, and sterilized in a high temperature and high pressure autoclave at 121 °C for 2 hours. After cooling to room temperature, the bacteria were inoculated. Take a 5mm uniform bacterium cake from the test tube for preserving the strain and inoculate it into a 500mL Erlenmeyer shaker flask filled with 150mL liquid PDA medium, and culture it with shaking at 170-180r / min at 21-23°C for 4 days as the first-grade bacteria kind. Use a sterile pipette to draw 5mL of bacteria from the first-level strain shake flask covered with mycelium balls and transfer it to a 500mL shake flask containin...

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Abstract

The invention provides a method for biosynthesizing organic selenium by using seafood mushrooms. The invention provides a seafood mushroom mutant strain obtained by ultraviolet mutagenesis capable of efficiently converting inorganic selenium in a culture medium into organic selenium, and the preservation number is CCTCC M 2019981. The fruiting and growth time of seafood mushrooms are short, the cultivation conditions are controllable, the amount of selenium addition is controllable, and the growth state is stable. The present invention preferably uses sodium selenate, sodium selenite and nano-selenium as the added forms, which have no effect on the biomass of the fruiting body of the seafood mushroom under the condition of a concentration of 40 mg / kg, and can promote the growth of the fruiting body of the seafood mushroom to a certain extent. Yield is improved, and selenomethylselenocysteine ​​can reach 39.4% of the total selenium content. During the cultivation process, the mycelium of the seafood mushroom grows rapidly, and the mushroom bag is filled in a short time. The seafood mushroom has a white and tender color and a high yield, which provides a powerful means for the preparation of organic selenium such as selenium methyl selenocysteine.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a method for biosynthesizing organic selenium by using seafood mushrooms. Background technique [0002] Seafood mushroom belongs to Basidiomycotina, Laminomycetes, Agaricales, White Mushrooms, and Amygium genus. It is a large woody saprophytic fungus with delicious meat and excellent taste. Seafood mushrooms are rich in amino acids, among which the content of lysine and arginine is higher than that of common mushrooms, which helps teenagers improve their intelligence. Seafood mushroom extract has a variety of physiologically active ingredients, among which fungal polysaccharides, purine, and adenosine can enhance immunity, promote antibody formation, prevent aging, fight cancer, and delay aging. At the same time, seafood mushroom is also a low-calorie, low-fat health food. [0003] Selenium is an essential trace element for the human body. The human body cannot synthesize selenium s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20A23L31/00A61K36/07A61K8/9728A61Q19/00
CPCA61K36/07A61Q19/00A23L31/00A61K8/9728A01G18/00A01G18/20
Inventor 郭岩彬胡婷赵桂慎丁惠惠改芳
Owner CHINA AGRI UNIV
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