A kind of plant anti-transpiration agent and preparation method thereof
An anti-transpiration agent and plant technology, which is applied in the field of plant anti-transpiration agent and its preparation, can solve the problems of hindering plants from absorbing oxygen and carbon dioxide emissions, affecting plant photosynthesis and respiration, and being easy to be rained down, etc., so that the process is easy Realization, low production cost and high product yield
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[0049] A preparation method of plant anti-transpiration agent, comprising the following steps:
[0050] A) making a rhamnolipid fermentation broth by fermenting a rhamnolipid-producing bacterial strain;
[0051] B) Separation of rhamnolipids from fermentation broth: After the fermentation is completed, a high-concentration rhamnolipid sample is obtained through double-effect vacuum concentration. After acid precipitation and ethyl acetate extraction, further concentration, and finally drying to obtain rhamnolipid samples.
[0052] C) Compounding the rhamnolipid with other components to obtain a plant anti-transpiration agent.
[0053] A preparation method for a fermented liquid containing rhamnolipids, comprising the following steps:
[0054] 1) Inoculating the rhamnolipid-producing bacterial strain into the seed culture medium at a ratio of 1-3% for expanded culture to obtain the seed bacteria fermentation liquid.
[0055] 2) Inoculate the fermented liquid of the seed bact...
Embodiment 1
[0062] Embodiment 1 (anti-transpiration strength experiment)
[0063] Select the local heather leaves in Zhoushan, harvest them in the morning, wash off the dirt, and dry them gently. Leaf roots were inserted into a 100 ml beaker containing 50 ml of phytoantitranspiration solution. Distilled water was used as a control. Place the beaker with the leaves in a room temperature of 26°C, and illuminate it with a 5,000-meter-candle fluorescent lamp for 24 hours, as shown in image 3 shown. Measure the amount of water loss per cup, use an area meter to find the leaf area, and calculate the transpiration intensity. The results are shown in Table 1 and figure 1 shown.
[0064] from figure 1 As can be seen from Table 1, the leaves added with rhamnolipid and polyglutamic acid solution after 24h have shown a better anti-transpiration effect, wherein 0.5% rhamnolipid+0.5% polyglutamic acid The transpiration intensity of amino acid is the smallest, that is, the anti-transpiration effe...
Embodiment 2
[0072] Embodiment 2: the acquisition of rhamnolipid
[0073] Preparation of seed medium: Mineral salt medium (MSM)+2% yeast powder (mass volume ratio), take Pseudomonas aeruginosa zs1.1 in the glycerol tube and insert the seed medium at a ratio of 2%, at 30°C , and cultured for 7 hours under the condition of a shaking table with a rotating speed of 180r / min.
[0074] Preparation of fermentation medium: fish oil 15g / L, camphor oil 15g / L, palm oil 10g / L, NaNO 3 5.43g / L, NH 4 NO 3 2.56g / L, Na 2 PO 4 10g / L, KH 2 PO 4 7.7g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 10.01g / L, trace element solution 3mL / L (FeSO 4 ·7H 2 O 18g / L; ZnSO 4 ·7H 2 O 3.0g / L; MnSO 4 2H 2 O 3.0g / L), yeast powder 0.5g / L.
[0075] Fill 30L of fermentation medium into a 50L tank, adjust the initial pH value of the medium to 7, and adopt vertical in-situ sterilization. The initial conditions are: rotating speed 300rpm, dissolved oxygen 45%, tank pressure about 0.04mPa.
[0076] The expanded seed bacter...
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