Preparation method of choline eutectic solvent and application of choline eutectic solvent in extraction of flavonoid compounds

Abstract

The invention discloses a preparation method of a choline eutectic solvent and application of the choline eutectic solvent in extraction of flavonoid compounds. The preparation process of the cholineeutectic solvent is as follows: taking proper amounts of choline chloride or choline hydroxide for uniformly mixing with a proper amount of an organic acid according to a ratio (mole number) of 1:1-1:10; putting a uniformly mixed reactant into a flat-bottomed flask with a proper size, and then adding a proper amount of water to initiate a reaction; and putting a magnetic stirrer at the temperature of 25-50 DEG C, putting the flat-bottomed flask on a magnetic stirrer, performing stirring for 1-24h at a proper rotating speed, after reactants in the bottle become transparent and uniform viscousliquid, transferring the liquid in the bottle into a rotary evaporator, and performing spin-drying on excessive moisture to obtain a viscous transparent liquid, namely a DES solvent finished product.The flavonoid compound extracted by the method has the characteristics of high purity, high extraction rate and simple and convenient process, the extraction time is shortened, the traditional solventis changed into an environment-friendly DES solvent which is easy to decompose, and the flavonoid compound is a real green chemical.

Description

technical field [0001] The invention belongs to the field of natural product extraction, in particular to a preparation method of a choline deep eutectic solvent and its application in extracting flavonoids from natural plants, and to a new extraction process of flavonoid natural products. Background technique [0002] Flavonoids are a class of natural compounds with a chromone ring, and are one of the important active ingredients in Chinese herbal medicine. The general structural formula of flavonoids is as follows: [0003] [0004] Flavonoids play an important role in traditional Chinese medicine: such as anti-inflammatory, analgesic, antibacterial and other effects, flavonoids are widely distributed in various green plants. my country is a big plant country and a big country of traditional Chinese medicine, with the world's largest resources of Chinese herbal medicine. With the advancement of science and technology and the development of modern technology and medical...

AI Technical Summary

Problems solved by technology

[0006] In recent years, most of the patents for extracting flavonoids from plants are traditional solvent extraction methods. For example, Chinese patent CN103349724A uses Fritillaria as the extraction raw material, ethanol as the main extraction solvent for reflux extraction, rotary evaporation to dryness to obtain the extract and low temperature storage; Chinese patent CN110302248 uses Chinese toon as the extraction material, uses ethanol as the extractant, uses choline chloride-1,2 propanediol as the reaction medium to extract the flavonoids in Chinese toon, spins to dryness to obtain the extract and preserves it at low temperature; in Chinese patent CN107628959A Taking choline as the basic raw material and adding a certain amount of AlCl3 or ZnCl2 to form a complex with it as a deep eutectic solvent DES to extract flavonoid glycosides at 90 ° C, this method not only reduces the cost, but also simplifies the process and obtains higher extraction rate, but the deep eutectic solvent DES contains Al, Zn and other metal elements that cannot be degraded by the environment, so it still has certain pollution to the environment; in the Chinese patent CN109701361A, choline chloride and polyhydric alcohols are used as raw materials to prepare A number of deep eutectic solvents DES, and using the DES to absorb SO2 and NO2 pollutants in toxic flue gas, this technology can transform some materials from a single solid phase to an inorganic liquid phase, and the adsorption conditions are mild and the process is simple. solves the problem of absorption and treatment of toxic and harmful substances in flue gas, but the deep eutectic solvent DES uses polyols as raw materials, but organic alcohol reagents will cause toxic residues

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