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Fluorescence resonance energy transfer system based on up-conversion nano material and sibo green and used for detecting phospholipase A2 in serum

A phospholipase and fluorescence technology, applied in analytical chemistry and nanometer fields, can solve the problems of increasing detection steps and costs

Active Publication Date: 2020-06-19
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods usually require the coupling of fluorophores and phospholipids to obtain functionalized fluorescent probes, which increases detection steps and costs

Method used

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  • Fluorescence resonance energy transfer system based on up-conversion nano material and sibo green and used for detecting phospholipase A2 in serum
  • Fluorescence resonance energy transfer system based on up-conversion nano material and sibo green and used for detecting phospholipase A2 in serum
  • Fluorescence resonance energy transfer system based on up-conversion nano material and sibo green and used for detecting phospholipase A2 in serum

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0051] Preparation of Liposome Nanoprobes Loaded with SG

[0052] 1) Preparation of SG-loaded liposome nanoprobes: The liposome nanoprobes were prepared by membrane hydration method. Dissolve dioleoylphosphatidylcholine (DOPC) in a mixed solution of chloroform and methanol and pour it into a round-bottomed flask; evaporate the solvent in a rotary evaporator to form a lipid film; after obtaining a uniform film, blow nitrogen to remove traces The organic solvent was stored overnight in a vacuum desiccator in the dark. Add Tris-HCl buffer solution II to the uniform film made above, fully rotate and hydrate in a water bath, and sonicate the water bath at room temperature; A polycarbonate membrane with a diameter of nm was extruded back and forth nine times to obtain a liposome suspension with a certain particle size. Its process is as follows figure 1 shown. The specific steps for the preparation of liposomes are as follows: 0.0197 g (0.025 mmol) of dioleoylphosphatidylcholine...

example 2

[0059] Construction of LRET-based upconversion fluorescent sensing platform

[0060] 1) Synthesis of rare earth-doped up-conversion nanomaterials: Take a 100 mL three-necked flask and add prepared rare earth stearates Y, Yb, Tm (molar ratio: 76.5%: 23%: 0.5%) for a total of 0.8 mmol, octadecene (ODE) 8 mL, NaF 28 mmol, oleic acid (OA) 12 mL, heated to 145 °C in an argon atmosphere, and kept for 35 min for dehydration and degassing; then rapidly raised to 315 °C, kept After reacting for 40 min, cool to room temperature, centrifuge the obtained product at 11000 rpm for 3 min, discard the supernatant, wash the precipitate with ethanol, cyclohexane, and distilled water until there is no NaF component, and dry it in vacuum at 60°C for later use to obtain oleic acid-coated β-phase UCNPs. NaYF synthesized by high temperature pyrolysis 4 : The TEM and XRD characterizations of Yb, Er nanocrystals fully demonstrate that the obtained crystal form is single, the size is uniform, and the...

example 3

[0065] Fluorescence Resonance Energy Transfer of UCNPs-SG

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Abstract

The invention discloses a fluorescence resonance energy transfer system based on an up-conversion nano material and sibo green, and the fluorescence resonance energy transfer system is used for detecting phospholipase A2 in serum. According to the invention, the rare earth doped up-conversion nano material is designed as a sensor of a fluorescent probe; and under the excitation of near-infrared light and in the presence of PLA2, UCNPs and SG generate an LRET effect, so that the blue light emission of the UCNPs at 476 nm is weakened, and the green fluorescence of the SG at 522 nm is enhanced. The quantitative detection of the activity of PLA2 is realized through the change of fluorescence ratios at 522 nm and 475 nm before and after the PLA2 is added. The sensor has the advantages that dueto the unique anti-Stokes luminescent property, the rare earth doped up-conversion luminescent nano material has the advantages of good photochemical stability and no autofluorescence. The experimental process is simplified. The method is based on the principle of fluorescence energy resonance transfer, has the advantages of high detection sensitivity and high detection specificity, and can be used for detecting phospholipase A2 in serum.

Description

technical field [0001] The invention relates to an analysis method based on the principle of fluorescence resonance energy transfer between upconversion nanomaterials and cyber green, and belongs to the field of analytical chemistry and nanotechnology. [0002] The present invention relates to constructing a non-labeled fluorescence resonance energy transfer method with rare earth-doped upconversion nanomaterials and liposome nanoprobes loaded with cyber green, which is used for phospholipase A in serum 2 detection. Background technique [0003] Phospholipase A 2 (PLA 2 ) is a hydrolase that can catalyze the diacyl group on the phospholipid glycerol molecule, and almost all cells contain PLA 2 , PLA is even found in bacteria and protozoa 2 . PLA can be activated by mechanical damage, endotoxin, angiotensin Ⅱ, etc. 2 Participate in physiological processes such as inflammatory response, membrane remodeling, and intercellular signal transduction. Recent studies have show...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/58
CPCG01N21/6428G01N33/582
Inventor 兰建明陈敬华李春艳吴芳罗登旺方垚沈毅萍
Owner FUJIAN MEDICAL UNIV
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