Enzymolysis composition, soybean meal enzymolysis method and preparation method of organic trace element feed additive

Abstract

The invention discloses a soybean meal enzymolysis composition. The soybean meal enzymolysis composition comprises the following components in parts by weight: 10 to 20 parts of calcium oxide, 3 partsof aspergillus oryzae, 5 parts of auxiliary enzyme, 0.5 part of riboflavin and 66 parts of compound protease, wherein the 5 parts by weight of auxiliary enzyme is composed of 1 part by weight of phytase (5000 IU / g), 2 parts by weight of xylanase (100000 IU / g), 0.5 part by weight of mannan (10000 IU / g), 0.5 part by weight of cellulase (10000 IU / g), 1 part by weight of amylase (10000 IU / g) and 1 part by weight of galactosidase (10000 IU / g), and the 66 parts by weight of compound protease is composed of 40 parts by weight of alkaline protease (100000 IU / g), 2 parts by weight of keratinase (500000 IU / g), 20 parts by weight of neutral protease (50000 IU / g), 3 parts by weight of acid protease (50000 IU / g) and 1 part by weight of peptidase.

Description

technical field [0001] The invention relates to the technical field of animal feed processing, in particular to an enzymatic hydrolysis composition, a method for enzymatic hydrolysis of soybean meal and a preparation method for organic trace element feed additives. Background technique [0002] Soybean meal is rich in nutritional value, its water-soluble protein can account for about 90%, and it is rich in amino acids, high in digestibility, and good in palatability. It is one of the most important plant protein sources for poultry and livestock. Soybean meal contains a variety of beneficial nutrients such as phospholipids, soybean isoflavones, peptides, and amino acids. [0003] Amino Acids: Mainly absorbed in the small intestine, each amino acid is absorbed one by one. Various amino acids are mainly absorbed through the active transport method that requires sodium ions to consume energy. The intestinal mucosa uses the sodium ion concentration gradient inside and outside th...

AI Technical Summary

Problems solved by technology

[0015] But its shortcomings are also obvious, mainly as follows: (1) The hydrolysis is not complete, the protein hydrolysis rate is about 20-40%, and there are many intermediate products

(2) The degradation of antigenic protein is low. Due to the large molecular weight and compact structure of glycinin and β-conglycinin, a large number of enzyme cleavage sites are hidden inside the protein molecule, which is difficult to be hydrolyzed by protease, generally around 5-20%. This is also the reason for limiting the application in feed. The taste of most enzymatic soybean meal is bitter and astringent, which seriously affects the palatability and dosage of animals.

Disadvantages: tryptophan with high nutritional value is almost completely destroyed, and reacts with aldehyde-containing compounds (such as sugar) to form a black substance called humin, so the hydrolyzate is black

The disadvantage is that the amino acids produced by hydrolysis undergo optical isomerization, and the products include D-type and L-type amino acids.

D-type amino acids cannot be decomposed and utilized by animals, so the nutritional value is halved; in addition, most of serine, threonine, lysine, cystine, etc. are destroyed, so alkaline hydrolysis is generally rarely used