Unlock instant, AI-driven research and patent intelligence for your innovation.

Fatty acid MALDI mass spectrometry imaging method based on in-situ derivatization and application thereof

A mass spectrometry imaging and derivatization reaction technology, which is applied in the field of fatty acid MALDI mass spectrometry imaging based on in-situ derivatization, can solve the problems of difficulty in simultaneous determination and low mass spectrometry response, and achieve simple derivatization reagents, improved sensitivity, and derivatization conditions. mild effect

Active Publication Date: 2020-08-25
SHANDONG ANALYSIS & TEST CENT
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely low mass spectrometric response of fatty acids in positive ion detection mode, it is difficult to achieve simultaneous determination of metabolites such as phosphatidylcholine, lysophosphatidylcholine, choline, and carnitine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fatty acid MALDI mass spectrometry imaging method based on in-situ derivatization and application thereof
  • Fatty acid MALDI mass spectrometry imaging method based on in-situ derivatization and application thereof
  • Fatty acid MALDI mass spectrometry imaging method based on in-situ derivatization and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Mass spectrometry imaging analysis of fatty acid derivatization in rat kidney tissue

[0044] (1) Take the rat kidney tissue and use the Thermo CryoStar microtome to make two coronal sections of the kidney tissue with a thickness of 12 μm for use;

[0045] (2) Transfer 2 kidney tissue sections to 2 ITO-indium tin oxide conductive glass slides, and place them in a vacuum desiccator to dry for 20 minutes;

[0046] (3) Precisely weigh 15mg of N,N,N-trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide in a 10mL volumetric flask, add 10mL acetonitrile: water (90 : 10, v / v) solution, vortex to mix well, and ultrasonic for 5 minutes to obtain N, N, N-trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide solution, which is ready for use ;

[0047] (4) Precisely weigh 6 mg of HBOt in a 10 mL volumetric flask, add 10 mL of acetonitrile: water (90:10, v / v) solution, vortex to mix, and ultrasonic for 5 minutes to obtain the HBOt solution for use;

[0048] (5) Precisely weigh 12 m...

Embodiment 2

[0060] (15) Targeted mass spectrometry data extraction of carnitine, choline, lysophosphatidylcholine, and phosphatidylcholine in derivatized kidney tissue slices by SCiLS Lab 2018b data processing software Example 2: Fatty acids in rat brain tissue Derivative mass spectrometry imaging analysis

[0061] (1) Take the rat brain tissue and use the Thermo CryoStar microtome to make a coronal section of the brain tissue with a thickness of 12 μm;

[0062] (2) Transfer the brain tissue section to an ITO-indium tin oxide conductive glass slide, and place it in a vacuum desiccator for 20 minutes;

[0063] (3) Precisely weigh 15mg of N,N,N-trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide in a 10mL volumetric flask, add 10mL acetonitrile: water (90 : 10, v / v) solution, vortex to mix well, and ultrasonic for 5 minutes to obtain N, N, N-trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide solution, which is ready for use ;

[0064] (4) Precisely weigh 6 mg of HBOt in a 10 mL volumetric flas...

Embodiment 3

[0073] Example 3: Synthesis of N,N,N-trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide

[0074] (1) Add 2.5 mM 1-(2-dimethylaminoethyl)piperazine to 20 mL of ether and vortex for 3 minutes;

[0075] (2) Add 0.5 mM methyl iodide to the above solution and vortex for 3 minutes;

[0076] (3) At room temperature, stir the above solution under a magnetic stirrer at 100rpm / min for 1 hour, then filter it, wash it with ether 3 times, and dry the solid matter on the filter paper to obtain N, N, N- Trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide.

[0077] Figure 4 It is the high resolution mass spectrum and product ion scanning mass spectrum of N,N,N-trimethyl-2-(piperazin-1-yl)ethane-1-ammonium iodide.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a fatty acid MALDI mass spectrum imaging method based on in-situ derivatization and application thereof, and belongs to the technical field of mass spectrum detection. The method at least comprises the following steps: spraying a derivatization reagent solution and a catalyst solution onto the surface of a prepared tissue slice, and carrying out a derivatization reaction ina closed environment containing saturated organic solvent gas to obtain a fatty acid derivatization product after the reaction is finished; carrying out matrix spraying on the derivatized tissue slice, and then carrying out MALDI mass spectrometry imaging analysis. According to the method, the detection sensitivity of the fatty acid in the tissue can be effectively improved, and simultaneous visual analysis of the fatty acid and metabolites such as phosphatidylcholine, lysophosphatidylcholine, choline, carnitine and the like in the biological tissue is realized, so that the method has good practical popularization and application values.

Description

Technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and relates to a MALDI mass spectrometry imaging method capable of highly sensitively detecting the spatial distribution of fatty acids in biological tissues in a positive ion mode, in particular to a fatty acid MALDI mass spectrometry imaging method based on in-situ derivatization and its application . Background technique [0002] Disclosure of the background information is only intended to increase the understanding of the overall background of the present invention, and is not necessarily regarded as an acknowledgement or in any form suggesting that the information constitutes the prior art known to those of ordinary skill in the art. [0003] Fatty acids are an important class of endogenous metabolites. In addition to being the main constituent molecules of lipids in biological membranes such as cell membranes, mitochondrial membranes and plasma membranes, fatty acids can also e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28C07D295/13
CPCG01N27/64G01N1/28C07D295/13
Inventor 孙成龙王晓刘伟耿岩玲
Owner SHANDONG ANALYSIS & TEST CENT