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Copper-iodine cluster compound @ photosensitizer composite nano particle and application thereof as X-ray photodynamic therapy drug

A composite nanoparticle and nanoparticle technology, applied in copper iodine cluster compound@photosensitizer composite nanoparticle and its application field as an X-ray photodynamic therapy drug, can solve problems such as human toxicity, achieve low toxicity and simple preparation method Easy, mild conditions

Active Publication Date: 2020-09-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main research on X-PDT is to develop new X-ray sensitizers. The currently reported X-ray nano-scintillators can be divided into sensitizers based on doped lanthanides, metal oxide (sulfur) compound sensitizers, transition metal Cluster complexes, etc., almost all reported X-ray sensitizers contain rare earths or heavy metals with high atomic numbers, which are extremely toxic to the human body

Method used

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  • Copper-iodine cluster compound @ photosensitizer composite nano particle and application thereof as X-ray photodynamic therapy drug
  • Copper-iodine cluster compound @ photosensitizer composite nano particle and application thereof as X-ray photodynamic therapy drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Cu 4 I 4 (P-(m-Tol) 3 ) 4 Preparation of @PpIX nanoparticles

[0026] Weigh 40 mg Cu 4 I 4 (P-(m-Tol) 3 ) 4and 4 mg of protoporphyrin (PpIX) were dissolved in 0.8 ml of chloroform, and ultrasonicated until completely dissolved to obtain solution A; 10 mg of sodium dodecylbenzenesulfonate and 30 mg of bovine serum albumin were dissolved in 10 ml of deionized water , sonicated until completely dissolved to obtain solution B; slowly drop solution A into solution B, and stir rapidly at room temperature for 1 h for pre-emulsification. Then the mixed solution was sonicated for 0.5 h to make it into a white microemulsion; the obtained white emulsion was transferred to a round-bottomed flask, and quickly rotated at 40 °C for 10 min by a vacuum pump, and then dialyzed to remove impurities in the solution, and finally obtained Cu 4 I 4 (P-(m-Tol) 3 ) 4 @PpIX nanoparticles.

Embodiment 2

[0027] Example 2: Cu 4 I 4 (P-(m-Tol) 3 ) 4 Preparation of @ZnPcS8 nanoparticles

[0028] Weigh 40 mg Cu 4 I 4 (P-(m-Tol) 3 ) 4 Dissolve in 0.8 ml of chloroform, sonicate until completely dissolved to obtain solution A; weigh 10 mg of sodium dodecylbenzenesulfonate and 30 mg bovine serum albumin and dissolve in 10 ml of deionized water, sonicate until completely dissolved to obtain solution B; Solution A was slowly added dropwise to solution B, and stirred rapidly at room temperature for 1 h for pre-emulsification. Then the mixed solution was ultrasonically treated for 0.5 h to make it into a white microemulsion; the obtained white emulsion was transferred to a round-bottomed flask, and quickly rotated at 40 °C for 10 min by a vacuum pump to prepare a 60 μmol / L ZnPcS8 aqueous solution, which was taken 1 mL was slowly added dropwise to the Cu 4 I 4 (P-(m-Tol) 3 ) 4 Nanoparticle aqueous solution, stirred for 24 h, and then dialyzed to remove impurities in the solutio...

Embodiment 3

[0029] Example 3: Cu 4 I 4 (P-(m-Tol) 3 ) 4 Characterization of @PpIX

[0030] Take Cu 4 I 4 (P-(m-Tol) 3 ) 4 10 μL of @PpIX nano-solution was dropped on the ordinary carbon support membrane, and dried overnight in a 30°C oven to make it fully dry. Put the product to be tested into the gold spraying machine, and put it into the sample compartment of the ultra-high resolution scanning electron microscope for testing after the gold spraying is completed. The results are shown in figure 1 . Such as figure 1 As shown in (a), Cu 4 I 4 (P-(m-Tol) 3 ) 4 The particle size of @PpIX nanoparticles is about 150nm.

[0031] Separately prepare Cu 4 I 4 (P-(m-Tol) 3 ) 4 @PpIX, Cu 4 I 4 (P-(m-Tol) 3 ) 4 and PpIX aqueous solution at 1 mg / mL, and then 200 μL each was placed in the sample cell of the X-ray fluorescence spectrometer, and the sample was excited by X-rays with 40 kV and 80 μA intensity, and the obtained fluorescence spectrum was as follows figure 2 . figur...

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Abstract

The invention discloses a copper-iodine cluster compound @ photosensitizer composite nano particle, and belongs to the technical field of medical functional materials. An X-ray sensitizer Cu4I4(P-(m-Tol)3)4 and a photosensitizer protoporphyrin PpIX or zinc octa-sulfonate phthalocyanine ZnPcS8 are compounded in a non-covalent mode through a micro-emulsion method, albumin and a surfactant namely sodium dodecyl benzene sulfonate are added, and water-soluble nano particles are prepared. Under X-ray irradiation, Cu4I4(P-(m-Tol)3)4 in the composite nano particles generates visible light, and then active oxygen (ROS) is generated by activating the photosensitizer to kill tumor cells. The preparation method is simple and easy to implement and mild in conditions, and the prepared nano material is low in price, good in stability, good in biocompatibility and low in toxicity, has a good killing effect on tumors, and has a wide application prospect in the field of X-ray photo-dynamics (X-PDT).

Description

technical field [0001] The invention belongs to the technical field of medical functional materials, and specifically relates to a copper-iodine cluster compound@photosensitizer composite nanoparticle for X-ray photodynamic therapy of tumors and a preparation method thereof. The composite nanoparticle is made of Cu 4 I 4 (P-(m-Tol) 3 ) 4 As an X-ray sensitizer, under X-ray irradiation, the energy is transferred to the photosensitizer through the FRET effect, and ROS is generated to kill tumor cells, which can be used for deep treatment of tumors. Background technique [0002] The traditional methods for treating tumors mainly include surgical resection, chemical drug therapy, and radiotherapy, but these methods have the disadvantages of large side effects, and patients suffer great pain during the treatment process. Photodynamic therapy (PDT) has attracted the attention of scientists due to its minimally invasive, good selectivity, and non-invasive treatment, and has beco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/14A61K47/02A61K47/42A61P35/00B82Y20/00B82Y30/00
CPCA61K9/143A61K9/146A61K41/0038A61K41/0071A61P35/00B82Y20/00B82Y30/00
Inventor 黄剑东王韧郝秀云
Owner FUZHOU UNIV