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Culture solution of low-temperature chlorococcum, culture method and application of low-temperature chlorococcum

A cultivation method, the technology of Chlorococcus, applied in the direction of microorganism-based methods, applications, chemical instruments and methods, etc., can solve the problems of affecting the self-purification speed of water bodies, slow growth of algae, and low concentration of living algae, so as to enhance the self-purification ability of water bodies , increase dissolved oxygen in water, and expand the effect of application fields

Pending Publication Date: 2020-09-08
王学良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the traditional common culture method, (due to low temperature and slow growth of algae), the culture time is long (15-30 days a batch), the number of live algae in the algae liquid is small, the concentration of algae is low, and there are many bacteria in the algae liquid, which cannot Meet the demand for high-quality bait for aquaculture animals, as well as the demand for the degradation and adsorption of harmful substances such as ammonia nitrogen, nitrite, phosphorus, and heavy metals in aquaculture water in low temperature periods; it cannot also purify livestock and poultry breeding wastewater, lakes, and rivers; it cannot meet the requirements The Requirements of Algae to Promote Energy Cycle and Material Cycle in Water Body
[0008] Through the above analysis, the existing problems and defects of the prior art are: when adopting common culture methods to cultivate Chlorococcus algae, at low temperatures, the growth rate of the algae is slow, and the degradation of ammonia nitrogen, nitrite, phosphorus, and heavy metals in the water body is relatively low. Slow, affecting the self-purification speed of water body
[0009] The difficulty in solving the above problems and defects is: due to the common culture method of algae, the algae species are not pure, and contain miscellaneous bacteria, which are easy to compete for nutrients in the culture solution, the culture period is long, and the concentration of viable algae in the cultured algae solution is low (Each milliliter of algae liquid contains about one million live algae)

Method used

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  • Culture solution of low-temperature chlorococcum, culture method and application of low-temperature chlorococcum
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  • Culture solution of low-temperature chlorococcum, culture method and application of low-temperature chlorococcum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The method for culturing Chlorococcum in this embodiment includes the following steps:

[0051] ① Preparation of culture fluid

[0052] The culture solution is prepared according to the following components: add 0.05g anhydrous glucose, 0.1g ammonium chloride, 0.02g dipotassium hydrogen phosphate, 0.01g magnesium sulfate heptahydrate, 0.01g sodium chloride, and 0.05 ferrous sulfate per liter of tap water. g. Calcium chloride 0.01g, boric acid 0.0001g, manganese chloride 0.0001g, zinc sulfate 0.0001g, sodium molybdate 0.0001g, copper sulfate 0.0001g, cobalt nitrate 0.0001g, adjust pH = 7.0 after mixing, that is 1L Culture medium

[0053] ②Cultivation of Chlorococcus

[0054] a Take 5 mL of the culture solution in a glass test tube, sterilize at 118°C for 12 minutes, after cooling to room temperature, pick 10 sterile Chlorococcum species, and put them into the culture solution under aseptic conditions, 12-25 Cultivate 22h under 1000-1500 Lux light at ℃ (preferably 25℃);

[0055]...

Embodiment 2

[0059] The method for culturing Chlorococcum in this embodiment includes the following steps:

[0060] ① Preparation of culture fluid

[0061] The culture solution was prepared according to the following components: 0.55g anhydrous glucose, 0.5g ammonium chloride, 0.1g dipotassium hydrogen phosphate, 0.05g magnesium sulfate heptahydrate, 0.05g sodium chloride, and 0.55 ferrous sulfate per liter of tap water. g. Calcium chloride 0.05g, boric acid 0.0005g, manganese chloride 0.0005g, zinc sulfate 0.0005g, sodium molybdate 0.0005g, copper sulfate 0.0005g, cobalt nitrate 0.0005g, adjust pH=7.0 after mixing, that is 1L Culture medium

[0062] ②Cultivation of Chlorococcus

[0063] a Take 5 mL of the culture solution in a glass test tube, sterilize it at 121°C for 21 min. After cooling to room temperature, pick 15 sterile Chlorococcus species and put them in the culture solution under aseptic conditions at 25°C. , Cultivate 24h under 1250 Lux light;

[0064] b. Take another 200 mL of the cu...

Embodiment 3

[0068] The method for culturing Chlorococcum in this embodiment includes the following steps:

[0069] ① Preparation of culture fluid

[0070] The culture broth was prepared according to the following components: add 1g anhydrous glucose, 1g ammonium chloride, 0.2g dipotassium hydrogen phosphate, 0.1g magnesium sulfate heptahydrate, 0.1g sodium chloride, 0.1g ferrous sulfate, per liter of tap water. 0.1g of calcium chloride, 0.001g of boric acid, 0.001g of manganese chloride, 0.001g of zinc sulfate, 0.001g of sodium molybdate, 0.001g of copper sulfate, 0.001g of cobalt nitrate, after mixing, adjust pH=7.0, which is 1L of culture medium ;

[0071] ②Cultivation of Chlorococcus

[0072] a Take 5 mL of the culture solution in a glass test tube, sterilize it at 125℃ for 30 min. After cooling to room temperature, pick 20 sterile Chlorococcum species and put them in the culture solution under aseptic conditions at 15℃ , Cultivate for 26h under 1500 Lux light;

[0073] b. Take another 200 mL...

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Abstract

The invention belongs to the technical field of algae culture, discloses a culture solution of low-temperature chlorococcum, and a culture method and application of the low-temperature chlorococcum. Firstly, a culture solution is prepared from the following components: anhydrous glucose, ammonium chloride, dipotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, ferrous sulfate, calcium chloride, boric acid, manganese chloride, zinc sulfate, sodium molybdate, copper sulfate and cobalt nitrate which are added into tap water; and then the chlorococcum is cultured by using the prepared culture solution. Each milliliter of algae liquid obtained through culture has a large quantity of live algae and is short in culture time, the requirements of aquaculture animal baits can be metunder a low-temperature condition in early spring, the content of ammonia nitrogen, nitrite and phosphorus in aquatic livestock and poultry culture water bodies, lakes and rivers can be rapidly reduced, and heavy metal is adsorbed. The dissolved oxygen in the water bodies is increased, self-purification capacity of the water bodies can be quickly enhanced, and the blank of market products for purifying the water quality by low-temperature microorganisms in early spring is filled up.

Description

Technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a culture solution of low-temperature Chlorococcus, and a cultivation method and application of low-temperature Chlorococcus. Background technique [0002] At present, algae are the basis of material circulation and energy circulation in water bodies. Algae can absorb carbon dioxide in the air under light conditions, use soluble ammonia nitrogen, nitrite, phosphorus, and heavy metals in the water body for reproduction and metabolism, and release oxygen to reduce The content of ammonia nitrogen, nitrite, phosphorus, and heavy metals in water is the biological basis for water self-purification. At the same time, the algae themselves are rich in nutrients, and can provide high-quality biological bait for aquatic animal seed in aquaculture, and can degrade harmful ammonia nitrogen, nitrite, and heavy metals in the water body, increase the dissolved oxygen in the wate...

Claims

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Application Information

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IPC IPC(8): C12N1/12A01K61/00A23K50/80A23K10/18C02F3/32C02F101/16C02F101/10C02F101/20C12R1/89
CPCC12N1/12A01K61/00A23K50/80A23K10/18C02F3/322C02F2101/16C02F2101/166C02F2101/105C02F2101/20Y02A40/81
Inventor 王学良夏江月王畅王宇晨王宇凡
Owner 王学良
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