Micro-ecological composite additive for aquatic products and application of micro-ecological composite additive

A composite additive, micro-ecological technology, applied in the application, additional food elements, bacteria used in food preparation, etc., can solve the problems of low quality of fermented feed, acid resistance, poor acid production ability, slow fermentation rate, etc. Immunity, strong acid resistance, high content of viable bacteria

Active Publication Date: 2020-09-18
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently commercially available lactic acid bacteria generally have the following disadvantages when applied to fermented feed: (1) poor acid resistance and acid production ability, slow fermentation rate; (2) poor antibacterial level, low quality of fermented feed

Method used

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  • Micro-ecological composite additive for aquatic products and application of micro-ecological composite additive
  • Micro-ecological composite additive for aquatic products and application of micro-ecological composite additive
  • Micro-ecological composite additive for aquatic products and application of micro-ecological composite additive

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation, screening and identification of embodiment 1 bacterial strain

[0029] 1. Sample source

[0030] Collected from the sediment of Litopenaeus vannamei farm in Jiaozhou City, Qingdao.

[0031] 2. Screening method

[0032] Take the sediment sample, add sterile saline, shake well for 1 hour (28°C, 150rpm), so that the bacteria in the sediment are fully dispersed in the saline, 10-fold gradient dilution, inoculated on 2216E, TSB and MRS solid medium respectively Incubate at 28°C for 24h-48h. Select a uniform and clear single colony and streak to purify the bacteria. The single colonies were named MST1, MST2, MST3, . . . , MST30 accordingly.

[0033] Use 6mol·L -1 hydrochloric acid to adjust the pH of the MRS liquid medium to 6.5, 5.0, 3.5 and 2.0, respectively. After activating the above-mentioned single colony obtained by isolation and purification and culturing to the logarithmic growth phase, inoculate 1% of the inoculum into MRS liquid medium with a pH of...

Embodiment 2

[0045] Embodiment 2 Lactobacillus plantarum YN01 antibacterial performance evaluation

[0046] Using Staphylococcus aureus and Vibrio parahaemolyticus as indicator bacteria, the differences in antibacterial effects between Lactobacillus plantarum YN01 and antibiotics were compared. The antibiotics used were penicillin, ceftriaxone, gentamicin, polymyxin B, neomycin, ofloxacin, doxycycline, and NA was used as the antibacterial test plate medium.

[0047] Spot the Lactobacillus plantarum YN01 bacterial solution on the plate coated with indicator bacteria, place the antibiotic sensitive paper, and incubate at a constant temperature of 28°C, observe within 48 hours whether there is an antibacterial transparent area or around the area where the drug sensitive paper is placed. The coverage area, the specific results are shown in Table 3.

[0048] The antibacterial situation of table 3 Lactobacillus plantarum YN01

[0049]

[0050] It can be seen from the data in Table 3 that th...

Embodiment 3

[0051] Embodiment 3 Lactobacillus plantarum YN01 produces protease ability assay

[0052] Insert Lactobacillus plantarum YN01 into MRS liquid medium, culture at 37°C for 24h, and continuously transfer and activate two generations, insert 1% ( / v / v) inoculum into 250mL MRS liquid medium, and culture at 37°C for 48h Centrifuge at 8000 r / min for 10 min at 4°C, collect the supernatant, and detect the protease activity in the supernatant by the following method.

[0053] The results show that the protease activity in the fermentation supernatant of Lactobacillus plantarum YN01 screened by the present invention is as high as 112U / mL, and unexpected technical effects have been achieved.

[0054] (1) Definition of enzyme activity: at 37°C, the amount of enzyme required to decompose bovine serum albumin to produce 1 μmol of tryptophan per minute is one enzyme activity unit.

[0055] (2) Enzyme activity assay method: take 50 μL concentration of 1% (w / v) bovine serum albumin (BSA), 450 μ...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a micro-ecological composite additive containing lactobacillus plantarum YN01 and an application of the micro-ecological composite additive in aquaculture. The lactobacillus plantarum YN01 is screened from bottom mud of a healthy litopenaeus vannamei culture pond, and has acollection number of CCTCC NO:M2020093. The micro-ecological composite additive can significantly improve the utilization rate of feed by cultured animals, promote the growth of the cultured animals,improve the immunity of aquatic animals, increase the full-cycle survival rate and improve animal body components, and can be widely applied to the field of aquatic feed.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a micro-ecological composite additive for aquatic products and its application. Background technique [0002] In recent years, my country's aquaculture industry has developed rapidly. In order to meet the growing consumer demand for aquatic products and increase the production of aquaculture animals, aquaculture enterprises have added antibiotics to animal feed, which has brought great contributions to the development of the entire aquaculture industry. . However, with the passage of time, the food safety problems caused by the use of antibiotics have become increasingly apparent, which has attracted great attention worldwide, and the concept that feed safety is equal to food safety has become a general consensus. People have begun to seek other alternatives and alternative technologies to ensure that the efficiency and effectiveness of animal husbandry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K50/80A23K10/12A23K20/174A23K20/163A23K20/28
CPCA23K10/18A23K50/80A23K10/12A23K20/174A23K20/163A23K20/28A23V2400/169
Inventor 倪梦丽魏万权王磊周怡郭本月刘广
Owner 青岛玛斯特生物技术有限公司
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