Rapid pretreatment method for simultaneously detecting glimepiride and metformin in blood plasma

A metformin and blood plasma technology, applied in the field of analysis, can solve the problems of long analysis time and complicated operation, and achieve the effects of low matrix interference, high sensitivity, and simplified sample processing steps

Active Publication Date: 2020-09-22
FUDAN UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a new pretreatment method for glimepiride and metformin biological samples in order to solve the existing pretreatment methods of glimepiride and metformin biological samples with complicated operation and long analysis time. A rapid pretreatment method for the enrichment and detection of glimepiride and metformin in plasma based on magnetic mesoporous graphene composites

Method used

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  • Rapid pretreatment method for simultaneously detecting glimepiride and metformin in blood plasma
  • Rapid pretreatment method for simultaneously detecting glimepiride and metformin in blood plasma
  • Rapid pretreatment method for simultaneously detecting glimepiride and metformin in blood plasma

Examples

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Embodiment 1

[0034] This example involves the simultaneous enrichment and detection of glimepiride and metformin in plasma samples by magnetic mesoporous graphene composite materials modified by sulfonic acid groups:

[0035] 1) Synthesis of magnetic mesoporous graphene composites modified with sulfonic acid groups:

[0036] (1) Weigh 300mg of graphene, add 100ml of concentrated nitric acid, place in a 60°C water bath, mechanically stir and react for 6 hours, cool to room temperature, wash with deionized water several times until neutral, and dry for later use;

[0037] (2) Get the acidified graphene 150mg prepared in (1) step, add ferric chloride hexahydrate 405mg, trisodium citrate 150mg, PEG-20000 1.0g, anhydrous sodium acetate 1.8g successively, ultrasonically disperse in 40ml ethyl alcohol In diol, react at 200°C for 10-12 hours, after cooling to room temperature, wash with water and absolute ethanol several times, and dry in vacuum for later use;

[0038](3) Get the magnetic graphen...

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Abstract

The invention belongs to the technical field of analysis, and relates to a rapid pretreatment method for detecting glimepiride and metformin in blood plasma, in particular to a rapid pretreatment method for enriching and detecting glimepiride and metformin in blood plasma based on a magnetic mesoporous graphene composite material. According to the method, the functionalized magnetic mesoporous graphene material is synthesized, and is used for the pretreatment process of the plasma sample and the method comprises the steps of extraction, cleaning, elution and detection, the sample treatment steps are obviously simplified, and the sample treatment time is shortened; the method can effectively eliminate interference caused by a matrix effect, is wide in linear range, good in recovery rate andhigh in sensitivity, can be used for simultaneously detecting the concentrations of glimepiride and metformin in blood plasma, and is further used for in-vivo pharmacokinetic analysis of glimepirideand metformin.

Description

technical field [0001] The invention relates to the field of analysis technology, in particular to a rapid pretreatment method for enrichment and detection of glimepiride and metformin in plasma based on magnetic mesoporous graphene composite material. Background technique [0002] The prior art discloses that glimepiride belongs to the third-generation sulfonylurea long-acting antidiabetic drug, and mainly plays a hypoglycemic effect by stimulating the secretion of endogenous insulin. Metformin is a biguanide oral hypoglycemic drug, which can improve insulin sensitivity by increasing the intake and utilization of peripheral sugar, reduce the production of glycogen, and reduce the intestinal absorption of sugar to achieve the purpose of lowering blood sugar. Based on the complementary hypoglycemic mechanism of glimepiride and metformin, the two are often used in combination clinically for the treatment of patients with type 2 diabetes mellitus whose hypoglycemic effect is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08G01N30/02B01J20/22B01J20/30B01J20/28
CPCG01N30/08G01N30/02B01J20/20B01J20/22B01J20/0229B01J20/28009B01J2220/4812B01J2220/4806
Inventor 李嫣江洁冰李佳佳朱婧琳佘晓健
Owner FUDAN UNIV
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