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Preparation for improving fish intestinal pathology injury

A intestinal and pathological technology, applied in the field of preparations for improving fish intestinal pathological damage, can solve problems such as intestinal damage in farmed fish

Inactive Publication Date: 2020-10-20
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem in the prior art that high-concentration histamine feed can cause intestinal damage to farmed fish, the present invention provides a preparation for improving intestinal pathological damage of fish that has a significant repair effect on intestinal damage caused by histamine

Method used

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  • Preparation for improving fish intestinal pathology injury
  • Preparation for improving fish intestinal pathology injury
  • Preparation for improving fish intestinal pathology injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In the experiment, white fish meal (histamine content 107.6 mg / kg) and soybean meal were used as the main protein sources to make basal feed C for peltago catfish experiment fish, and 1000 mg / kg exogenous histamine was added to basal feed C to make a diet containing Feed H with a high concentration of histamine, and fed the experimental fish with feed H for 30 days, and established the experimental fish intestinal injury model (the detailed formula composition of feed C and feed H is shown in Table 1, wherein the nutritional composition of the feed is analyzed according to GB / T- 5009 series method, the determination of histamine content is carried out according to GB / T 5009.45-2003 / 4.4 method). The evaluation method for establishing the pathological injury model of experimental fish is as follows: ≥80% of the samples (intestines) of the experimental fish in group H have obvious pathological injury and the activity of intestinal injury indicator enzymes has a significant ...

Embodiment 2

[0051] According to the experimental design of Example 1, a culture experiment was carried out for 56 days. At the end of the experiment, the fish were fasted for 1 day, and the test fish were lightly anesthetized with MS-222, and blood was drawn from the tail vein of 5 fish in each tank. The collected blood was placed in a foam box filled with ice cubes, and after being completely coagulated, it was centrifuged at 4000 g for 10 min (4°C). The prepared serum samples were temporarily stored in liquid nitrogen and stored at -80°C until analysis.

[0052] Serum biochemical indicators such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and diamine oxidase (DAO) activity were purchased from Nanjing Jiancheng Bioengineering Institute, and measured with a Thermo full-wavelength microplate reader. The effects of each feed group on the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and diamine oxidase (DAO) of experimental fish ...

Embodiment 3

[0054] According to the experimental design of Example 1, the intestinal tissue samples (5 fishes per tank) of the experimental fishes in the 56-day breeding experiment were collected and fixed in neutral formalin for 48 hours. The samples were dehydrated by alcohol gradient, transparentized in xylene, and embedded in paraffin, and sliced ​​with a thickness of 4 μm. After the sections were dewaxed with xylene, stained with hematoxylin-eosin and mounted with neutral gum, they were observed under an optical microscope (model: OLYMPS CX31) and images were collected.

[0055] image 3 The microstructure of the intestinal tissue of the peliotus observed after HE staining. The results of microscopic observation showed that the folds of the foregut intestine tissue in group C were closely arranged, the cell structure was normal, the epithelial cells were arranged neatly, the microvilli layer was relatively neat, and the size of vacuoles on the nucleus was relatively uniform; the num...

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Abstract

The invention discloses a preparation for improving fish intestinal pathology injury. The preparation disclosed by the invention is prepared from the following components in parts by weight: 1 part ofmangosteen extract and 3 parts of stevia rebaudian extract. When the preparation is added into a feed in proportion or mixed with the feed for use, the preparation has remarkable repairing and improving effects on damaged intestinal tracts of cultured animals and has no adverse effect on the production performance of aquatic animals. The preparation is added into feed according to the proportionof 0.1%, so that the intestinal injury of pelteobagrus fulvidraco caused by histamine can be remarkably repaired; the intestinal health condition of the ricefield eel fed by the iced fresh fish can beobviously improved by mixing and feeding the preparation according to the proportion of 0.3%. The preparation is wide in raw material source, convenient and easy to purchase, easy to obtain and low in cost; the preparation is simple to prepare and operate without special instruments and equipment; and the preparation is simple and easy to use, high in applicability, free of residues after being used and environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a preparation for improving fish intestinal pathological damage. Background technique [0002] As an important inflammatory factor, histamine is closely related to physiological activities such as immunity and inflammation resistance. Animal raw materials rich in histidine in aquatic feed (such as fish meal) are susceptible to histidine decarboxylase-producing bacteria (such as Proteus morganii, Achromobacter histamine, Escherichia coli) during production and storage. , Streptococcus, Staphylococcus, etc.) pollution, so that free histidine decarboxylation reaction to generate histamine. Histamine, as the most toxic biogenic amine, can cause serious harm to the intestinal health of aquatic animals by binding to specific histamine receptors. The intestinal tract is not only an important digestive and absorption organ of the body, but also the largest immune organ ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P1/00A01K61/13A23K50/80A23K10/30
CPCA61K9/0056A61K36/185A61K36/28A61K2236/15A23K10/30A23K50/80A01K61/13A61P1/00A61K2300/00Y02A40/81
Inventor 许国焕成艳波张丽李志朋谭文俊郭莹姿
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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