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Saline-alkaline tolerant enterobacter cloacae and production method and application of viable bacterium preparation of saline-alkaline tolerant enterobacter cloacae

A technology of Enterobacter cloacae and live bacteria preparation, applied in the field of agricultural biology, can solve the problems of limited control effect, no obvious promotion effect on pepper growth and the like

Active Publication Date: 2020-10-23
SHANDONG ZOETICLAND BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antagonistic bacteria such as Bacillus and Pseudomonas fluorescens, and antagonistic fungi such as Trichoderma, Aspergillus and Pythium oligandrum have been used for the biological control of pepper blight, but due to the limited control effect, they have no obvious promotion effect on pepper growth. Failed to be widely promoted in agricultural production

Method used

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  • Saline-alkaline tolerant enterobacter cloacae and production method and application of viable bacterium preparation of saline-alkaline tolerant enterobacter cloacae
  • Saline-alkaline tolerant enterobacter cloacae and production method and application of viable bacterium preparation of saline-alkaline tolerant enterobacter cloacae
  • Saline-alkaline tolerant enterobacter cloacae and production method and application of viable bacterium preparation of saline-alkaline tolerant enterobacter cloacae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening of Enterobacter cloacae ZTS-10

[0031] (1) Screening of saline-alkali-tolerant strains

[0032] Enterobacter cloacae ZTS-10 was isolated from capsicum rhizosphere soil. The soil was sampled in Bincheng District, Binzhou City, Shandong Province in September 2018, which is moderately saline-alkali soil. The specific separation method is as follows: mix the soil sample evenly, weigh 5g, put it into a conical flask filled with 95mL sterile water and 10 glass beads, shake it at 37°C and 180rpm for 30min. Take 1 mL of soil suspension for 10 -1 —10 -7 Serial concentration gradient dilution, and then take 10 -5 、10 -6 、10 -7 The three dilutions were spread on the plate containing the selection medium, and cultured upside down at 37°C for 2 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured upside down at 37°C for 2 days. Pick a single colony and transfer it to the slant of the preservation medium te...

Embodiment 2

[0048] Example 2 Identification of Enterobacter cloacae ZTS-10

[0049] (1) Morphological and physiological and biochemical characteristics

[0050] Colony and thalline characteristics of the Enterobacter cloacae ZTS-10 strain: cultured on NA medium at 37° C. for 48 hours, the diameter of the colony is about 0.5-0.8 mm, white and translucent, round, smooth, raised, moist, sticky Thick, shiny, uniform texture, neat edges; the bacteria are short rod-shaped (such as figure 1 shown). The NA medium is nutrient agar medium, and its formula is 3 g of beef extract, 10 g of peptone, 5 g of NaCl, 20 g of agar, 1000 mL of water, and pH 7.0.

[0051] The physiological and biochemical characteristics of the Enterobacter cloacae ZTS-10 bacterial strain: Gram-negative bacillus; Catalase test positive, citrate utilization test positive, malonate utilization test positive, nitrate reduction test positive, The V-P test was positive; the starch hydrolysis test was negative, the D-glucose ferm...

Embodiment 3

[0055] Example 3 Preparation of Enterobacter cloacae ZTS-10 bacterial agent

[0056] The preparation method of the Enterobacter cloacae ZTS-10 bacterial strain bacterial agent comprises the following steps:

[0057] 1) Activation of strains: Transfer the low-temperature-preserved Enterobacter cloacae ZTS-10 strains to the slant of NA medium test tubes, and culture them at 37° C. for 24 hours for activation. The NA medium is nutrient agar medium, and its formula is: beef extract 3g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH7.0.

[0058] 2) Erlenmeyer flask seed preparation: Scrape the activated Enterobacter cloacae ZTS-10 lawn with an inoculation loop, inoculate it in NB medium, and incubate at 37°C for 24 hours. The NB medium is nutrient broth medium, and its formula is: 3g of beef extract, 10g of peptone, 5g of NaCl, 1000mL of water, and pH7.0.

[0059] 3) Preparation of seed tank strains: Transfer the seeds of the triangular flask to a 10L seed tank containing 6-7L ...

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Abstract

The invention discloses saline-alkaline tolerant enterobacter cloacae and a production method and application of a viable bacterium preparation of the saline-alkaline tolerant enterobacter cloacae. The saline-alkaline tolerant enterobacter cloacae ZTS-10 is separated from rhizosphere soil of chili peppers in saline-alkaline land, the preservation number of the bacterial strain in the China GeneralMicrobiological Culture Collection Center (CGMCC) is CGMCC No.20181, and the preservation date is 3 July, 2020. The bacterial strain is high in saline-alkaline tolerance and high in field planting rate in the saline-alkaline soil, has the capacity of producing IAA, degrading phosphorus and degrading potassium, can promote growth of the chili peppers, has good antagonism effects on pathogenic bacteria of chili pepper epidemic diseases, can be used for producing microorganism fertilizers especially microorganism fertilizers special for the chili peppers in the saline-alkaline land, can reduce the generation of chili pepper epidemic diseases, and can increase yield and quality.

Description

technical field [0001] The invention relates to a saline-alkali-resistant Enterobacter cloacae and a production method and application thereof, belonging to the field of agricultural biotechnology. Background technique [0002] my country is a big pepper producing country, and the national pepper planting area can reach 2 million hectares, accounting for more than 12% of the vegetable planting area. Capsicum has strong adaptability, many varieties, and high economic benefits. It has become an important pillar product for increasing farmers' income and rural development in many areas. Capsicum blight is the most serious disease threatening capsicum production, and it has occurred widely in capsicum growing areas around the world. If the field conditions are suitable, the disease can cause about 30% yield reduction, sometimes even up to 100%, seriously restricting economic development. [0003] Pepper blight is mainly a devastating fungal disease caused by Phytophthora capsi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00A01P3/00C05G3/00C05G3/60C12R1/01
CPCC12N1/20A01N63/20C05G3/00C05G3/60C12R2001/01C12N1/205
Inventor 孙中涛辛寒晓赵升远孙国科陈君君韩志真徐艳平
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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