Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum

A high-activity technology of Lactobacillus plantarum, applied in the biological field, can solve the problems of industrialized production of antimicrobial peptides, achieve good industrialized production potential, and increase output

Inactive Publication Date: 2020-11-10
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems existing in the prior art, the present invention provides a fermentation medium and fermentation conditions for the production of highly active antimicrobial peptides by Lactobacillus plantarum CGMCC5297, so as to overcome the problem that the Lactobacillus plantarum CGMCC5297 antimicrobial peptides have not been industrialized in the prior art.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum
  • Method for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum
  • Method for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Determination of Antimicrobial Peptide Activity of Lactobacillus plantarum CGMCC5297

[0016] 1. Drawing of rifampicin standard potency curve

[0017] Using rifampicin as a control, a standard curve of rifampicin was made, and the antibacterial potency of Lactobacillus plantarum CGMCC No 5297 was defined by the titer of rifampicin standard substance with the same antibacterial effect on Listeria monocytogenes . In the potency detection plate, add respectively 2ug / ml, 3ug / ml, 4ug / ml, 5ug / ml, 6ug / ml, 7ug / ml, 8ug / ml, 9ug / ml, rifampicin of 10ug / ml (GENE company , item number: 19-1059) standard item, take Listeria monocytogenes CVCC1595 as indicator bacteria, bacterial concentration is that 107cfu / ml connects in the LB medium that agar content is 0.8%. Add 100ul to each Oxford cup, diffuse at 4°C for 24 hours, and incubate at 30°C in the dark for 24 hours. Take the logarithmic value of rifampicin micrograms as the abscissa, and the diameter of the antibacterial zone as the...

Embodiment 2

[0022] Optimizing Antimicrobial Peptide Production Medium

[0023] 1. Optimize the carbon and nitrogen sources in the medium

[0024] The remaining components and contents of MRS in the fermentation medium were unchanged, and eight glucose, sucrose, lactose, whey, cornstarch, and molasses were selected as carbon sources with a concentration of 20g / L to determine the appropriate carbon source, and the best The concentration of the carbon source is optimized. The optimization results show that glucose is the optimal carbon source for producing antimicrobial peptides, and the optimal concentration of glucose fermentation to produce Lactobacillus plantarum antimicrobial peptides is 40g / L.

[0025] With 40g / L glucose as the best carbon source, soybean cake powder, yeast extract, corn steep liquor, yeast powder, and tryptone were selected as nitrogen sources with a concentration of 20g / L, and the appropriate nitrogen source was determined, and its concentration was determined. opt...

Embodiment 3

[0036] Optimizing fermentation conditions with LiFlus GM automatic hexaplex fermenter

[0037] The optimal medium was used for the fermentation of Lactobacillus plantarum antimicrobial peptides, and the LiFlus GM automatic hexaplex fermenter was used to optimize the inoculation volume, ventilation volume, and rotational speed. The experimental results are shown in Table 2. LiFlus GM automatic hexaplex fermenter can realize simultaneous fermentation of six tanks. For optimization experiments of fermentation conditions, this equipment can achieve efficient and accurate results. From the analysis of the extreme R value of the experimental results, it can be seen that the order of the influence of the three factors on the fermentation of the Lactobacillus plantarum CGMCC5297 antimicrobial peptide is: A (aeration volume) > C (inoculum volume) > B (rotational speed). The optimal combination of fermentation conditions is: aeration ratio 5.5vvm, rotation speed 120r / min, inoculum size ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fermentation condition for producing high-activity antibacterial peptide by fermenting with lactobacillus plantarum CGMCC5297. An optimized fermentation culture medium formula comprises 10-60g / L of glucose, 8-12g / L of casein peptone and 3-7.5 g / L of yeast powder, and the optimized fermentation condition of a fermentation tank is as follows: the ventilation ratio is 4.7-6.4 vvm, the rotating speed is 100-150r / min, and the inoculum size is 1.5-3.5%. The pH value of the culture medium is 5.8-6.2. According to the method, the mass ratio of a carbon source to a nitrogen source in the culture medium is optimized, besides, the addition amount of inorganic salt and the addition amount of a surfactant are controlled, the yield of the antibacterial peptide is greatly increased, the titer of the obtained antibacterial peptide is 1.4 times that of the antibacterial peptide before being optimized, and good industrial production potential is shown.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing high-activity antibacterial peptides by fermentation of plant lactobacillus. Background technique [0002] Food safety is an important issue worldwide, especially in developing countries like my country. In recent years, the number of patients with foodborne diseases has remained high, and food poisoning caused by microorganisms is very serious. Adding chemical preservatives is an important food preservation method used by humans, but long-term studies have found that excessive consumption of synthetic preservatives has adverse effects on blood pressure, heart, kidneys, etc., and even some chemical preservatives are carcinogenic, teratogenic and easy to cause food poisoning etc. Thermal sterilization is also an important method to kill microorganisms during food processing, but for some low-temperature products, if the heating temperature is too high, the colo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12N1/20C12R1/25
CPCC12N1/20C12P21/00
Inventor 宋诙郑雯徐健勇谭明郑宏臣
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com