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Lactobacillus plantarum Lp2 and application thereof

A technology of Lactobacillus plantarum and probiotics, applied in the field of microorganisms

Active Publication Date: 2020-11-13
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no anti-inflammatory probiotic strains or related products with independent intellectual property rights in my country. Therefore, Lactobacillus plantarum Lp2 was isolated and purified from the naturally fermented food that Chinese residents often eat by plate streaking method and its anti-inflammatory effect was studied. The development of probiotic strains and their products with independent intellectual property rights that are suitable for consumption by Chinese residents is of great significance to the development of my country's probiotic industry and the promotion of national health

Method used

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  • Lactobacillus plantarum Lp2 and application thereof
  • Lactobacillus plantarum Lp2 and application thereof
  • Lactobacillus plantarum Lp2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening and identification of Lactobacillus plantarum Lp2

[0024] 1. Screening of bacterial strains

[0025] The commercially available Northeast sauerkraut was isolated from lactic acid bacteria in the laboratory; -1 Gram-positive strains were selected by observing the colony morphology with Gram staining. ; The selected strains were cultured in the liquid MRS medium at 37°C for 24 hours, inoculated according to the inoculum amount of 3% of the culture medium, and continuously activated and passaged three times to ensure the vigorous growth of the bacteria.

[0026] The liquid MRS medium contains 10.0 g of peptone, 10.0 g of beef extract, 5.0 g of yeast extract, diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ] 2.0 g, glucose (C 6 h 12 o 6 ·H 2 O) 20.0 g, Tween 80 1.0 mL, sodium acetate (CH 3 COONa·3H 2 O) 5.0 g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0 g, magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g, manganese sulfate (MnSO ...

Embodiment 2

[0031] Example 2 Lactobacillus plantarum Lp2 tolerance test

[0032] 1. Determination of the growth curve and acid production capacity of Lactobacillus plantarum Lp2

[0033] During the cultivation of Lactobacillus plantarum Lp2, samples were taken every 2 h, and the culture solution was analyzed as follows: the total number of bacteria was measured by spectrophotometry (wavelength 600 nm) to measure the absorbance value (OD); the pH value was measured by an acidity meter. The growth curve and pH curve of the strain are shown in image 3 ;Depend on image 3 It can be seen that 0-2 h is the lag phase of the strain, and the number and pH value of the strain do not change. After 2 h, it enters the logarithmic phase, and the number of bacteria increases linearly and rapidly. With the increase of the number of bacteria, the metabolite lactic acid is also more When the pH value decreased to about 2, the growth of lactic acid bacteria stagnated due to acid inhibition, and gradually...

Embodiment 3

[0060] Example 3. Test of adhesion ability of Lactobacillus plantarum Lp-2 to Caco-2 cells

[0061] 1. Culture of Caco-2 cells

[0062] Inoculate Caco-2 cells into cell culture flasks and culture in DMEM (Dulbecco's modified eagle medium) containing 10% fetal bovine serum + 1% penicillin-streptomycin double antibody solution at 37°C, 5% CO 2 Under these conditions, the degree of cell polymerization can reach 90% to 100% after being cultured for 5-6 days; Caco-2 cells in good growth state were inoculated on a 24-well plate (pore diameter 16 mm, effective membrane area 1.9 cm 2 ), the seeding density was 0.5×10 5 / well, 37°C, 5% CO 2 The cells were cultured for 21 days under the same conditions, and the medium was changed every other day. After observing the growth of the cells under an inverted microscope for about 21 days, the cells formed a compact monolayer.

[0063] 2. Preparation of Lactobacillus plantarum Lp2 bacterial suspension

[0064] The strain was inserted into ...

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Abstract

The invention discloses a lactobacillus plantarum Lp2. The preservation number of the lactobacillus plantarum Lp2 is CCTCC NO: M 2019935. A probiotic solid beverage comprises the following componentsin parts by weight: 10 parts of lactobacillus plantarum freeze-dried powder, 5-10 parts of isomaltooligosacharide, 5-10 parts of soybean oligosaccharide and 5-15 parts of fruity powder. The preservation number of the lactobacillus plantarum is CCTCC NO: M 2019935. The invention also discloses application of the lactobacillus plantarum Lp2 in preparation of anti-enteritis drugs. Results show that the lactobacillus plantarum Lp2 has good acid stress tolerance, cholate stress tolerance, pathogenic bacteria infection resistance and high intestinal tract colonization capacity, and has a protectiveeffect on LPS-induced acute inflammation; the lactobacillus plantarum Lp2 strain is superior to other strains in in-vitro probiotic function evaluation and in-vivo indexes of mice, and it is shown that lactobacillus plantarum Lp2 shows obvious strain specificity in the aspect of anti-inflammation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Lactobacillus plantarum Lp2 and its application. Background technique [0002] In daily life, some bad eating habits, such as high fat and excessive intake of alcohol, etc., will lead to the increase of lipopolysaccharide in the body. When excess lipopolysaccharide enters the blood circulation and contacts the host, it can trigger a systemic inflammatory response, leading to multiple organ damage, shock, and even death. In fact, patients with obesity, diabetes, cardiovascular disease, and nonalcoholic fatty liver disease showed increased levels of LPS in the blood, all of which are associated with inflammation. Studies have confirmed that most of the endotoxins in patients with these diseases do not originate from the infection itself, but are caused by the transfer of endotoxins in the gastrointestinal tract. [0003] Lipopolysaccharide (LPS) pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/39A23L33/135A23L33/125A61K35/747A61P1/00C12R1/25
CPCA23L2/39A23L33/135A23L33/125A61K35/747A61P1/00A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3204A23V2250/28
Inventor 王玉华陈奕颖王宇孙海月管午阳田园张楠
Owner JILIN AGRICULTURAL UNIV
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