Green process for extracting ultralow-viscosity low-M/G-value sodium alginate from kelp
A technology of sodium alginate and ultra-low viscosity, which is applied in the field of extracting sodium alginate with ultra-low viscosity and low M/G value, can solve the problems of high energy consumption, cost, environmental pollution, etc., improve purity and yield, and promote dissolution. , the effect of improving rigidity and compressive strength
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Embodiment 1
[0036] A green process for extracting ultra-low viscosity and sodium alginate with low M / G value from kelp, comprising the following steps:
[0037] The first step, kelp pretreatment
[0038] Wash and dry the kelp and grind it into 60-mesh powder with a drug grinder for later use. Take 10g of dried kelp and add 20 times distilled water to soak at room temperature for 2 hours;
[0039] The second step, enzymatic hydrolysis of kelp
[0040] Add 0.280g cellulase, 0.280g pectinase, 0.140g protease to the pretreated kelp solution, then add citric acid buffer solution to control the pH of the system to 4, enzymolysis at 50°C for 18 hours, then keep at 90°C 5min to inactivate the enzyme;
[0041] The third step, ultrasonic digestion
[0042] Add 2% of the solution mass of sodium carbonate to adjust the pH to 9, apply ultrasound at 150w for 30min, and carry out alkali digestion at 50°C to obtain a pasty viscous liquid. Filter by centrifugation and take the supernatant.
[0043] T...
Embodiment 2
[0047] A green process for extracting ultra-low viscosity and low M / G value sodium alginate from kelp, comprising the following steps:
[0048] The first step, kelp pretreatment
[0049] Wash and dry the kelp and grind it into 50-mesh powder with a drug grinder for later use. Take 10g of dried kelp and add 20 times distilled water to soak at room temperature for 1.5h;
[0050] The second step, enzymatic hydrolysis of kelp
[0051] Add 0.280g cellulase, 0.280g pectinase, 0.140g protease to the pretreated kelp solution, then add citric acid buffer solution to control the pH of the system to 4, enzymolysis at 50°C for 15 hours, then keep at 90°C 5min to inactivate the enzyme;
[0052] The third step, ultrasonic digestion
[0053] Add 2% of the solution mass of sodium carbonate to adjust the pH to 9, apply ultrasound at 150w for 120min, and carry out alkali digestion at 50°C to obtain a pasty viscous liquid. Centrifugal filtration, take the supernatant;
[0054] The fourth st...
Embodiment 3
[0063] A green process for extracting ultra-low viscosity and low M / G value sodium alginate from kelp, comprising the following steps:
[0064] The first step, kelp pretreatment
[0065] Wash and dry the kelp and grind it into 150-mesh powder with a drug grinder for later use. Take 10g of dried kelp and add 20 times distilled water to soak at room temperature for 1 hour;
[0066] The second step, enzymatic hydrolysis of kelp
[0067] Add 0.315g cellulase, 0.315g pectinase, 0.070g protease to the pretreated kelp solution, then add citric acid buffer solution to control the pH of the system to 5, enzymolysis at 60°C for 12 hours, then keep at 90°C 5min to inactivate the enzyme;
[0068] The third step, ultrasonic digestion
[0069] Add 4% of the solution mass of sodium carbonate to adjust the pH to 11, apply ultrasound at 150w for 70min, and carry out alkali digestion at 40°C to obtain a pasty viscous liquid. Centrifugal filtration, take the supernatant;
[0070] The fourth...
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