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Pochonia chlamydosporia for preventing and treating root-knot nematode disease, compound powder and application

A root-knot nematode disease and powder technology, applied in nematicides, applications, pest control, etc., can solve problems such as water source, soil and crop pollution, impact on human health, and decline in the quality of agricultural products, so as to promote crop growth, Improve nematode effect and reduce damage

Active Publication Date: 2020-12-11
青岛和协生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most nematocides currently used in production are highly toxic chemical pesticides, which not only affect human health, but also pose a threat to the environment
Long-term use of a large amount of pesticides will easily cause pesticide resistance of pests, soil compaction, pollution of water sources, soil and crops, and decline in the quality of agricultural products

Method used

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  • Pochonia chlamydosporia for preventing and treating root-knot nematode disease, compound powder and application
  • Pochonia chlamydosporia for preventing and treating root-knot nematode disease, compound powder and application
  • Pochonia chlamydosporia for preventing and treating root-knot nematode disease, compound powder and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation and Screening of Puccinia pachychinensis HX413

[0031] About 500 g of root knot nematodes were collected from the soil of a tomato planting greenhouse in Jiangjiazhuang Village, Bolan Town, Jimo District, Qingdao City, Shandong Province, and about 500 g of tomato roots were infested. In a funnel of water, connect a rubber tube to the bottom of the funnel and drain it to the nematode receiving test tube. After 1 day, clamp the rubber tube, take out the receiving test tube, centrifuge at 6000 rpm, discard the supernatant to obtain 1.5 ml of nematode suspension; take 0.3 ml of nematode suspension The turbid liquid was coated on a 2% agar horizontal plate, and cultivated at 26-28°C for 15-20 days; when colonies formed, a small amount of mycelium was picked from the edge of the colony and moved to a new PDA plate, cultivated for 7 days, and the colony morphology was observed. Carry out preliminary identification, and transfer the colony to CMA plate for purificati...

Embodiment 2

[0034] According to the formula in Table 1, microbial protection agents 1-3 were prepared.

[0035] Among them, zeolite powder, humic acid, corn starch, sucrose, sodium chloride and soil temperature are all commercial products. Wherein, the carbohydrate or protein substance is instant soybean powder.

[0036] Preparation example Zeolite powder (g) Humic acid (g) Corn starch (g) Sucrose (g) Sodium chloride (g) soil temperature (g) 1 30 10 20 0.5 0.1 0.1 2 45 20 30 1.2 1 1 3 50 30 40 2 1.5 1.5

Embodiment 3

[0038] A kind of compound microbial powder containing chlamydia thaliana for controlling root-knot nematode disease

[0039] According to the formula in Table 2, composite microorganism powders 1-4 were prepared, wherein the carbohydrate or protein substance was instant soybean powder, and the protective agent was the protective agent 1 obtained in Example 2.

[0040] Table 2

[0041]

[0042] A kind of preparation method of the compound microorganism powder that contains the chlamydia thaliana for preventing and treating root-knot nematode disease comprises the steps:

[0043] Mix the hyphae of Chlamydomonas thaliana HX413 obtained by self-cultivation and isolation with the purchased Paecilomyces lilacinus powder, protective agent and instant soybean powder, adjust the moisture to 15-20%, and then dry at below 30°C. It is obtained until the moisture is below 8%.

[0044] Table 3 shows the content of Paecilomyces lilacinus and Chlamydomonas pachypuscinia HX413 of the obta...

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Abstract

The invention discloses Pochonia chlamydosporia for preventing and treating a root-knot nematode disease, compound powder and application, and belongs to the technical field of microbial agents. The Pochonia chlamydosporia is a Pochonia chlamydosporia HX413 strain, and is preserved in the China General Microbiological Culture Collection Center on June 17th, 2020, the preservation number is CGMCC:20207, and the classification name is Pochonia chlamydosporia. The Pochonia chlamydosporia has excellent performance in the aspect of preventing and treating the root-knot nematode disease, and is compounded with traditional nematicide paecilomyces lilacinus, the two bacteria achieve a synergistic effect, the nematode killing effect is greatly improved, and meanwhile the effects of nitrogen fixation, fertilizer increase, rooting, root protection and crop growth promotion are achieved; the compound microbial powder acts on crops in agricultural production, the yield of the crops can be increased, and the yield increasing rate is 22% or above.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculants, and in particular relates to a strain of Chlamydomonas puccinia for preventing and treating root-knot nematode disease, a composite powder and application thereof. Background technique [0002] Root-knot nematode is a highly specialized omnivorous plant pathogenic nematode. After continuous cropping, root-knot nematode produces a large number of eggs in plant roots and hatches, resulting in large-scale crop yield reduction. Root-knot nematode disease leads to a reduction in food production and a huge loss of economic income worldwide every year. [0003] Puccinia thaliana is an endoparasitic fungus that can parasitize eggs, larvae, females, etc., and can significantly reduce the damage of plant nematodes such as root-knot nematodes, cyst nematodes, and stem nematodes of various crops. Puccinia pachytonia can not only inhibit the infection of nematodes, but also promote the growth of...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P5/00A01P21/00C12R1/645C12R1/79
CPCC12N1/14A01N63/30C12R2001/645C12N1/145Y02A40/10
Inventor 宗睿梁寒冰刘鲁民倪海平
Owner 青岛和协生物科技有限公司