A plant growth-promoting strain xn-k13 and its application
A plant pathogenic bacteria, a technology for promoting plant growth, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve problems such as reduced utilization rate, and achieve the effects of promoting growth and development, increasing the content of available potassium, and promoting plant growth
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Embodiment 1
[0037] Isolation and identification of plant growth promoting strain xn-k13
[0038] 1.1 isolation of plant growth promoting strain xn-k13
[0039] The separation of plant growth promoting strain xn-k13 includes three steps: sampling, enrichment, preliminary screening and re screening, as follows:
[0040] 1.1.1 sampling
[0041] Take samples from the soil of Guangxi Medicinal Botanical Garden (E108 ° 19 ', N22 ° 51'), put them into clean sampling bags, mark them, and store them in a 4 ℃ refrigerator as rhizosphere soil samples.
[0042] Primary screening and enrichment
[0043] Weigh 1.0g rhizosphere soil sample, put it into a test tube containing 9ml sterile deionized water, fully shake and mix, 37 ℃, 200R / min, shake and culture for 30min, and dilute it according to ten step gradient to obtain the diluent; In order to isolate salt tolerant bacteria, 10g / L NaCl was added as salt stress, 100ul diluent was absorbed and coated on beef extract peptone solid medium containing 10g ...
Embodiment 2
[0062] Accurate characterization of potassium hydrolysis by plant growth promoting strain xn-k13:
[0063] The solubilization ability of Enterobacter ludwigii xn-k13 to insoluble potassium was preliminarily evaluated through the improved potassium solubilizing bacteria selection medium: the strain isolated and purified from 1.1.3 was pricked in the center of the improved potassium solubilizing bacteria selection medium containing potassium feldspar as insoluble potassium source with a sterile toothpick, and cultured in a 37 ℃ incubator for 5 days, with three repetitions in each group, Observe the occurrence of potassium dissolving circle, measure the diameter of colony (d) and potassium dissolving circle (d), and the results are shown in Table 2. Calculate the ratio of potassium dissolving circle to colony diameter（ Figure 4 )； From table 2 and Figure 4 It can be concluded that the ratio of potassium dissolving circle to colony diameter of environmental stress resistant strain xn-...
Embodiment 3
[0067] Quantitative analysis of potassium hydrolysis of plant growth promoting strain xn-k13:
[0068] Potassium standard solution: accurately weigh 1.9068g of dried (dried at 105 ℃ for 4-6 hours) analytical pure KCl, dissolve it in 200ml deionized water, fix the volume to 1L, shake well to obtain 1000mg / L potassium, suck the above solution into a 25ml to 250ml volumetric flask, fix the volume and shake well to obtain 100mg / L potassium standard solution.
[0069] 3% sodium tetraphenylborate solution: weigh 3G sodium tetraphenylborate and dissolve it in 100ml deionized water. Add 10 drops of 0.2mol / l sodium hydroxide solution to adjust the pH to 8-9. After standing overnight, filter it with dense filter paper and store it in a brown bottle.
[0070] 0.05mol / l borax solution: weigh 19.07g borax and dissolve it in 1000ml deionized water.
[0071] Formaldehyde EDTA solution: weigh 2.5G disodium EDTA salt and dissolve it in 20ml 0.05mol / l borax solution, add 80ml 37% formaldehyde, ...
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