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Bone tissue repair material as well as preparation method and application thereof

A technology for repairing materials and bone tissue, which is applied in the field of bone tissue repairing materials and its preparation. It can solve the problems of high partial or complete shedding of mineralized silk protein particles, a single method for obtaining products, and large capacity. It achieves low requirements for reaction equipment, Avoid scattering or leaking, the effect of increased elasticity

Active Publication Date: 2021-01-01
HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese patent "Mineralized Silk Protein Material and Its Preparation Method" (publication number CN1241654A) discloses a mineralized silk protein material and its preparation method, wherein the organic component is degummed silk protein, and the minerals are calcium phosphorus salt crystals. The specific method It is to add calcium ion-containing solution and phosphate ion-containing solution to the water-soluble degummed silk protein solution under stirring, adjust the pH value of the reaction system, keep stirring for a while, let the system stand still, repeat centrifugation, wash the precipitate, and then put Precipitation is vacuum-dried to obtain the product; the system has the problems of non-constant temperature, large capacity, pH deviation from the most stable value of hydroxyapatite, and a single method for obtaining the product
At the same time, there is a higher risk of partial or complete detachment of fine mineralized silk protein particles from the bone graft during use

Method used

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  • Bone tissue repair material as well as preparation method and application thereof
  • Bone tissue repair material as well as preparation method and application thereof
  • Bone tissue repair material as well as preparation method and application thereof

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preparation example Construction

[0033] The preparation method of the bone tissue repair material provided by the present invention comprises the following steps:

[0034] (1) In the liquid phase system of pH=10~12, the soluble calcium salt and soluble phosphate (or phosphoric acid) with the molar ratio of Ca and P of 5:3 undergo in situ co-precipitation reaction on the natural polymer A, and the precipitation reaction occurs. After cleaning, drying, grinding and sieving, the hydroxyapatite-natural polymer A nanocomposite particles of different specifications are formed;

[0035] (2) The hydroxyapatite-natural polymer A nanocomposite particles and the natural polymer B are uniformly mixed in a liquid phase system, then placed in a mold for vacuum freeze-drying, and then soaked and dried in absolute ethanol to obtain Bone tissue repair material with fixed morphology;

[0036] Among them, the natural polymer A and the natural polymer B are the same or different.

[0037] Specifically include the following ste...

Embodiment 1

[0049] (1) Weigh 75.3 g of soluble silk fibroin, put it into 3L of purified water at a constant temperature of 40°C, and dissolve for 10 minutes under stirring at 150-1500 rpm to obtain a silk fibroin solution.

[0050] (2) Weigh 342.1 g of trisodium phosphate dodecahydrate, put it into the silk fibroin solution obtained in step (1) at one time, stir at 150 to 1500 rpm until the solution is clear, put in 354.2 g of calcium nitrate tetrahydrate at one time, and continue to stir;

[0051] (3) after a large amount of milky white precipitation appears in the reaction system, add 1.5L of concentrated ammonia water (NH 3 The concentration is 25-28 wt%), ensure that the pH value of the system is about 11, and stir for 3 hours to make it fully react.

[0052] (4) After the completion of the reaction, the stirring was stopped, and the reaction product was aged for 20 hours at a constant temperature of 40°C in the reaction vessel.

[0053] (5) Pour out the aged reaction product.

[0...

Embodiment 2

[0062] (1) Weigh 60.5 g of soluble silk fibroin, put it into 3 L of purified water at a constant temperature of 37°C, and dissolve for 10 minutes under stirring at 1000 rpm to obtain a silk fibroin solution.

[0063] (2) put 342.1g potassium dihydrogen phosphate into the silk fibroin solution prepared in step (2) at one time, stir at a rotating speed of 500~1000rpm until the solution is clear, and then put 354.2g anhydrous calcium chloride into it at one time, Continue to stir the reaction.

[0064] (3) After a large amount of milky white precipitation appears in the reaction system, sodium hydroxide solution (concentration is 3M) is added in batches to ensure that the pH value of the system is about 11, and the stirring is continued for 3 hours to make it fully react.

[0065] (4) After the reaction is completed, the stirring is stopped, and the reaction product is aged for 16 hours under the constant temperature condition of 37°C in the reaction vessel.

[0066] (5) Pour ou...

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Abstract

The invention discloses a bone tissue repair material and a preparation method and application thereof. The preparation method comprises the steps that in a liquid phase system with the pH value of 10-12, soluble calcium salt and soluble phosphate or phosphoric acid with the molar ratio of Ca to P being 5: 3 are subjected to an in-situ co-precipitation reaction on a natural polymer A, and hydroxyapatite natural polymer A nano-composite particles are formed after precipitate is dried and ground; and hydroxyapatite natural polymer A nano-composite particles are mixed uniformly with a natural polymer B solution, a mixture is put into a mold, vacuum freeze drying is carried out, soaking is carried out with absolute ethyl alcohol, and drying is carried out to obtain the bone tissue repair material. The bone tissue repair material is loose and porous, hydroxyapatite is not prone to leaking from the bone tissue repair material, and the bone tissue repair material is good in water absorption,soft after absorbing water and good in elasticity and can be used for common bone defect repair in the oral implant department, the oral repair department, the orthopedics department and other surgerydepartments. The preparation method is simple and convenient to operate, easy to automate, clean in reagent and low in cost.

Description

technical field [0001] The invention relates to the field of bone tissue engineering materials, in particular to a bone tissue repair material and its preparation method and application. Background technique [0002] At present, due to the huge market demand, bone tissue substitute materials are a hot spot in the research and application of biomedical materials. The repair effect of endogenous bone tissue is good, but the promotion and use of this therapy is limited due to the shortage of alternative materials; while the repair of exogenous bone tissue has obvious immune rejection, and may also introduce potential pathogens and other toxic side effects. It can be seen that it is of great practical significance to study and prepare an efficient and safe artificial bionic bone tissue replacement material. [0003] Natural bone tissue is a uniform and orderly biological complex composed of inorganic minerals (mainly nano-hydroxyapatite) and natural polymers (mainly collagen). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/32A61L27/46A61L27/56
CPCC01B25/325A61L27/46A61L27/56C01P2002/72C01P2004/03C01P2006/10C01P2006/17A61L2430/02A61L2430/12
Inventor 邓红兵蒋朝俊
Owner HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
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