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A kind of lignan compound in purslane and its extraction and separation method and application

A technology of lignans and separation methods, which is applied in the field of extraction and separation of lignans, can solve the problems of low structural novelty, and achieve the effects of environmentally friendly process methods, simple and fast operation methods

Active Publication Date: 2022-05-20
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed

Method used

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  • A kind of lignan compound in purslane and its extraction and separation method and application
  • A kind of lignan compound in purslane and its extraction and separation method and application
  • A kind of lignan compound in purslane and its extraction and separation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Extraction and separation of lignans from purslane and its extraction and separation method.

[0037] A kind of lignan compound extracted and separated from purslane, the molecular formula of this compound is C 20 h 18 o 6 , and named olealignan A, its chemical structure is as follows:

[0038] ,

[0039] Described compound is called olealignan A, and table 1 is the NMR data of this new compound: 1 H-NMR with 13 C-NMR in deuterated DMSO.

[0040] Table 1. NMR data of the new compounds of the present invention.

[0041] .

[0042] The structural identification of a kind of lignan compound extracted and separated from purslane refers to Figure 1-10 , the biological structure identification and deduction of the compound are as follows.

[0043] Oleralignan A: yellow powder, easily soluble in methanol. HR-ESI – - MS gives m / z 353.1021 [M-H] – The quasi-molecular ion peak has a molecular weight of 353.1031. combine 1 H-NMR, 13 According to C-NMR...

Embodiment 2

[0053] Example 2 Antitumor effect of the new compound of the present invention.

[0054] 1 main material.

[0055] 1.1 Drugs and reagents.

[0056] The new compound used in the experiment was prepared by the above method with a purity of 90-99%, weighed accurately, and diluted with DMSO to the solution required for each dosage group below. DMEM high glucose medium, fetal bovine serum (Hyclone Company, USA); penicillin, streptomycin (Hangzhou Sijiqing Company).

[0057] 1.2 Cell lines.

[0058] Human colon cancer cell Caco-2, human breast cancer cell MCF-7, human gastric cancer cell BGC-823, human lung adenocarcinoma cell SPC-A1, human liver cancer cell BEL-7402, human cervical cancer cell Hela-229, ovarian cancer cell Ho-8910, human oral epidermoid carcinoma cells KB (Shanghai Cell Bank, Chinese Academy of Sciences).

[0059] 1.3 Grouping.

[0060] Divided into control group, experimental group and zero adjustment group (culture solution containing DMSO vehicle).

[0061...

Embodiment 3

[0070] Example 3 Anticholinesterase effect of compounds of the present invention.

[0071] 1 main material.

[0072] 1.1 Drugs and reagents.

[0073] The lignan compounds used in the experiment were prepared by the above method with a purity of 90-99%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology), 5,5' -Dithiobisnitrobenzoic acid (DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and acetylthiocholine iodide (ATCI, Dalian Meilun Biological Co., Ltd. Technology Co., Ltd).

[0074] 1.2 Grouping.

[0075] Divided into negative control group, positive control group and experimental group, each group.

[0076] 2. Experimental method.

[0077] 2.1 Sample preparation.

[0078] Precisely weigh the sample and 1 mg of physostigmine, respectively, and use methanol as the solvent to prepare five gradient concentrations of (5, 10, 20, 30, 50 μM / mL). Accurately weigh 7...

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Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method and application. The invention provides a lignan compound olealignan A extracted from purslane and its extraction and separation method and application. The ester compound is sequentially extracted by alcohol decoction, silica gel column chromatography, polyamide column chromatography, ODS medium-pressure column and Sephadex LH-20 purification, liquid phase separation preparation, the extraction and separation method is simple, fast, and environmentally friendly, and the purity of the compound obtained by this method is relatively high. Pharmacological experiments have proved that the obtained compound has anti-tumor and anti-cholesterol Alkaline esterase, so the lignan compound olealignan A extracted from purslane and its salts and derivatives can be used as natural products to develop new traditional Chinese medicines, and have broad medical application prospects.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method and application, specifically a lignan compound extraction and separation method and its application. Background technique [0002] Purslane (Portulaca oleracea L.), also known as horse amaranth, longevity vegetable, ant vegetable, is an annual herb of the family Portulacaceae. Purslane is widely distributed and rich in resources. It is one of the 78 wild plants with the same origin of medicine and food stipulated by the Ministry of Health of my country. Purslane is recorded in the 2015 edition of "Pharmacopoeia of the People's Republic of China". It has the functions of clearing heat and detoxifying, cooling blood and stopping bleeding, and stopping dysentery. , hemorrhoids, metrorrhagia, etc. [0003] Modern phar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/92A61P35/00A61P43/00A23L33/105
CPCC07D307/92A61P35/00A61P43/00A23L33/105A23V2002/00A23V2200/30A23V2200/308A23V2250/21A23V2300/14A23V2300/38A23V2300/50Y02A50/30
Inventor 张文洁徐纹英锡相
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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