Culture medium used for bovine in vitro fertilization and method for improving bovine in vitro fertilization

Abstract

The invention relates to a culture fluid used for bovine in vitro fertilization and a method for improving bovine in vitro fertilization. The method for bovine in vitro fertilization comprises the following steps: washing the mature cumulus-oocyte complex in the fertilization medium, then transferring it to the fertilization medium, putting it into an incubator, and putting it into an incubator for subsequent use; tube, thawed in a water bath at 37°C, inject the semen into the centrifuge tube containing the semen preparation culture medium, centrifuge and discard the supernatant; add the semen preparation culture medium to the above centrifuge tube, resuspend the sperm pellet, and take an appropriate sperm suspension for spermatogenesis Counting; add the calculated volume of sperm suspension into the fertilization culture drop filled with oocytes, and put the culture plate into the incubator to incubate the sperm and eggs together, and complete the in vitro fertilization operation, ready for in vitro culture of embryos. The involved fertilization culture fluid and semen preparation culture fluid contain ingredients such as sodium pyruvate and heparin. The method of the invention can significantly improve the efficiency of bovine in vitro fertilization.

Description

technical field

[0001] The invention belongs to the technical field of animal reproduction, and relates to a technology for agricultural-animal husbandry and veterinary reproduction, in particular to a method for bovine in vitro fertilization. In addition, the present invention also relates to the use of related working test solutions used in bovine in vitro fertilization. Application in fertilization. The bovine in vitro fertilization method of the invention has excellent technical effect. Background technique

[0002] In Vitro Fertilization (In Vitro Fertilization) or (external fertilization) refers to the technology in which mammalian sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is referred to as IVF in English. Because it is inseparable from embryo transfer technology (ET), it is also referred to as IVF-ET. In biology, animals obtained by transplanting in vitro fertilized embryos into mothers are calle...