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Tobacco allelopathic autotoxic substance degrading bacteria, compound bacteria agent wettable powder and its application

A technology of wettable powder and self-poisoning substances, which is applied in the fields of application, disinfectant, animal repellent, etc., can solve the problems of low breeding success rate, poor control effect, and drug resistance of bacteria, and achieve the goal of reducing the stimulating growth effect Effect

Active Publication Date: 2022-05-17
HUBEI TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the strategies for the prevention and control of bacterial wilt mainly include chemical control, biological control and agricultural control. Among them, chemical control has the disadvantages of poor control effect in the later stage, and long-term application will lead to the resistance of bacteria. The low success rate of variety selection is the key to agricultural control. problem

Method used

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  • Tobacco allelopathic autotoxic substance degrading bacteria, compound bacteria agent wettable powder and its application
  • Tobacco allelopathic autotoxic substance degrading bacteria, compound bacteria agent wettable powder and its application
  • Tobacco allelopathic autotoxic substance degrading bacteria, compound bacteria agent wettable powder and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Allelopathic autotoxic substances

[0028] Tobacco field soil that has been continuously cropped for ten years is taken. The soil extract is extracted with 80% ethanol solution, separated according to acidic, neutral, and alkaline components, and detected by GC-MS. The alkaline components are mainly long-chain alkanes. Not listed in 1, the substances in Table 1 are the detected substances in the acidic and neutral components, the quantitative method adopts the relative response factor method, and calculates according to the ratio of the peak area size to the peak area of ​​the internal standard compound. Six substances were obtained, and the content of allelochemicals increased year by year through continuous cropping.

[0029] Table 1 The content percentage (%) of allelopathic autotoxic substances

[0030]

[0031] 2. Screening of bacteria degrading allelopathic autotoxic substances

[0032] The soil used for bacteria screening was collected from the sewage out...

Embodiment 2

[0049] 1. Antagonistic effect of degrading bacteria on R. solanacearum

[0050] Ralstonia solanacearum HF1-1 was provided by the Microbiology Laboratory of School of Life Sciences, Hubei University.

[0051] The degrading bacteria of various allelopathic and autotoxic substances were activated with LB medium, and Ralstonia solanacearum was activated with NA medium. The NA plate containing the coated R. solanacearum was perforated on the plate, and the degrading bacteria liquid was added into the wells, and the antagonism between the degrading bacteria and R. solanacearum was detected by the plate confrontation experiment.

[0052] figure 2 Antagonism between dibutyl phthalate-degrading bacteria (left), benzoic acid-degrading bacteria (right) and R. solanacearum. image 3 Antagonism between diisobutyl phthalate degrading bacteria and Ralstonia solanacearum.

[0053] The experimental results show that dibutyl phthalate degrading bacteria, diisobutyl phthalate degrading bacte...

Embodiment 3

[0066] 1. Preparation of composite bacterial agent wettable powder

[0067] On the basis of Examples 1 and 2, in order to facilitate the transportation and storage of the degrading bacteria, the respective degrading bacteria were prepared into composite bacterial agents, and the control effect of the composite bacterial agents on tobacco bacterial wilt was observed.

[0068] Preparation of compound bacterial agent:

[0069] Bacillus megaterium ED2, Bacillus amyloliquefaciens YZ1, Bacillus sp.N01, Bacillus sp.N013, Bacillus amyloliquefaciens SD2, Bacillus amyloliquefaciens B4 were fermented by soybean meal medium.

[0070] The various additives selected for wettable powder are as follows:

[0071] Carrier: bentonite, diatomaceous earth, white carbon black, talc.

[0072] Dispersant: sodium lignosulfonate, sodium pyrophosphate, sodium hexametaphosphate, PEG8000.

[0073] Wetting agent: saponin powder, pulling powder, washing powder.

[0074] High temperature stabilizers: sod...

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Abstract

The invention belongs to the technical field of tobacco bacterial wilt prevention and control, and in particular relates to tobacco allelopathic and autotoxic substance-degrading bacteria, composite bacterial agent wettable powder and applications thereof. The present invention screens degrading bacteria capable of degrading allelopathic autotoxic substances, and finally aims at allelopathic autotoxic substances dibutyl phthalate, diisobutyl phthalate, benzoic acid, 3-methoxy-4- hydroxybenzoic acid, p-hydroxybenzaldehyde or 2,4-di-tert-butylphenol to obtain six strains of degrading bacteria, which can be applied to the prevention and treatment of tobacco bacterial wilt to avoid the production of environmentally harmful substances through physical and chemical methods, and at the same time The degrading bacteria use allelochemical substances as nutrients to reduce the content of allelopathic autotoxic substances in the soil, thereby reducing the growth-stimulating effect of allelopathic autotoxic substances on R. solanacearum and cutting off the effect of allelopathic autotoxic substances on R. solanacearum. chemotaxis-inducing effect.

Description

technical field [0001] The invention belongs to the technical field of tobacco bacterial wilt prevention and control, and in particular relates to tobacco allelopathic and autotoxic substance-degrading bacteria, composite bacterial agent wettable powder and applications thereof. Background technique [0002] Tobacco bacterial wilt has attracted much attention in tobacco leaf production, and the control of tobacco bacterial wilt has become one of the research hotspots in the field of tobacco leaf production. At present, the strategies for the prevention and control of bacterial wilt mainly include chemical control, biological control and agricultural control. Among them, chemical control has the disadvantages of poor control effect in the later period, and long-term application will lead to the resistance of bacteria, and the low success rate of variety selection is the key to agricultural control. problem. Biological control methods have become a hot spot in the control of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N25/14A01P1/00C12R1/11C12R1/07
CPCA01N63/22A01N25/14
Inventor 杨春雷余君杨勇李春黎杨小琼陈守文马昕蔡冬波王勤
Owner HUBEI TOBACCO SCI RES INST