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Application of lactobacillus plantarum in promoting liver regeneration

A technology of Lactobacillus plantarum and liver regeneration, which is applied in the field of microorganisms, can solve the problems of unclear influence on liver regeneration ability, and achieve the effects of promoting liver function recovery, liver regeneration ability, and liver regeneration

Active Publication Date: 2021-04-16
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of probiotics on liver regenerative capacity after partial hepatectomy is unclear

Method used

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  • Application of lactobacillus plantarum in promoting liver regeneration
  • Application of lactobacillus plantarum in promoting liver regeneration
  • Application of lactobacillus plantarum in promoting liver regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the cultivation of Lactobacillus plantarum

[0021] (1) MRS medium: glucose 20g, beef extract 10g, peptone 10g, yeast extract 5g, anhydrous sodium acetate 5g, dipotassium hydrogen phosphate 2g, diammonium hydrogen citrate 2g, manganese sulfate 0.25 g, magnesium sulfate 0.5g , Tween-80 1ML, (add 20g of agar to the solid medium) 1000mL of distilled water, adjust the pH to 6.2, and sterilize by autoclaving at 121°C for 20min.

[0022] (2) Activation of lactic acid bacteria

[0023] Pick an appropriate amount of screened lactic acid bacteria and inoculate them into 10mL MRS for primary culture, and place them in a 37°C constant temperature incubator for 20 hours, then add them to 90mL MRS liquid medium for secondary culture, and place them in a 37°C constant temperature incubator for 24 hours . After centrifugation, the supernatant medium was removed, and the pellet was reconstituted with 1 ml of physiological saline, and then plated. The results show that ...

Embodiment 2

[0024] Example 2: Liver regeneration experiment

[0025] 1. Construction and grouping of animal models with partial liver resection

[0026] Carry out 70% liver resection with reference to the Higgns-Anderson method, and specific process is as follows: 4% chloral hydrate intraperitoneal anesthesia (0.6-0.8ml / 100g); Rat is fixed on the operating table, after abdomen iodine disinfection, median incision is opened Abdomen, free perihepatic ligament; Gently move the abdominal viscera to the left, wrap it in wet gauze (soaked in normal saline), fully expose the liver; use 6.0 silk surgical knotting method to ligate the corresponding liver part, after confirming that there is no bleeding, use a micro The lobes of the liver were cut and removed; finally, the peritoneal and skin incisions were continuously sutured with 3.0 silk sutures to keep warm. Partial resection of 70% or less of the liver can be performed according to the above method.

[0027] The specific groups are as follo...

Embodiment 3

[0056] Example 3: Liver function recovery experiment after partial hepatectomy

[0057] 1. Construction and grouping of animal models with partial liver resection

[0058] Same as Example 2

[0059] 2. Method

[0060] 5ml of blood was collected from the inferior vena cava, and after 15 minutes at room temperature, 3000rpm, the serum was collected, and an automatic biochemical analyzer was used to measure alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (total bilirubin) , TTB) and other indicators reflect the damage of liver function. see results Figure 5 .

[0061] Figure 5 It showed that in terms of protein synthesis, the concentrations of total protein (TP), albumin (ALB) and globulin (GLo) in the serum of rats in the partial hepatectomy operation group, AR113 Lactobacillus protection group and AR113 Lactobacillus treatment group for 3 days were normal Groups (CK3 and CK7) and prosthetic hand group (JSH) both decreased. Compared wit...

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Abstract

The invention relates to the technical field of microorganisms, and discloses an application of lactobacillus plantarum AR113 in promoting liver regeneration. The invention provides a new application of lactobacillus plantarum AR113 in promoting liver regeneration and liver function recovery after liver partial resection, which comprises the following steps: evaluating the liver regeneration capacity after resection, observing and comparing pathological sections, detecting a liver cell regeneration marker ki67, detecting important regulatory factors of liver regeneration and the like, and verifying that compared with a control group, the lactobacillus has obvious activity of promoting liver regeneration; meanwhile, the evaluation on the liver function verifies that the lactobacillus plantarum can also promote the liver function recovery of the injured liver, and the experimental conclusion shows that the lactobacillus plantarum AR113 can provide appropriate probiotics for the liver resection patient to adopt special nutrition support in the perioperative period and promote the liver regeneration capacity and the liver function recovery.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of Lactobacillus plantarum in promoting liver regeneration. Background technique [0002] Liver cancer is the second malignant tumor with the highest cancer mortality rate in the world. Hepatectomy is an effective method for the treatment of huge liver tumors, and it is very important to protect the remnant liver function and promote liver regeneration after surgery. However, after pathological resection, whether the residual liver can regenerate and function will directly affect the patient's recovery and survival. It has been found in clinical experiments that the use of specific nutritional support therapy during the perioperative period can significantly reduce liver damage and complications in patients. [0003] Probiotics are a class of microorganisms that have basically no toxic side effects on humans and mammals, and when given in sufficient doses...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P1/16
Inventor 谢纯良彭源德艾连中朱作华龚文兵周映君夏永军严理胡镇修
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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