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A strain of Lactobacillus rumen for relieving colitis and its application

A technology for Lactobacillus stomach and colitis, applied in the field of microorganisms, can solve problems such as affecting the success of treatment, and achieve the effects of reducing damage, reducing disease activity index, and broad market prospects

Active Publication Date: 2022-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used therapeutic drugs are: sulfasalazine salicylic acid preparations such as Edissa, mesalamine, etc.; corticosteroids commonly used are prednisone or dexamethasone, but long-term use of the above drugs may lead to hypertension, diabetes , osteoporosis and other diseases, thereby affecting the success of treatment

Method used

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  • A strain of Lactobacillus rumen for relieving colitis and its application
  • A strain of Lactobacillus rumen for relieving colitis and its application
  • A strain of Lactobacillus rumen for relieving colitis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Example 1: Screening, identification, cultivation, observation and preservation of Lactobacillus ruminis CCFM1141

[0084] 1. Screening

[0085] Take 1g of healthy human feces samples from Shawan, Xinjiang, apply them on MRS solid medium after gradient dilution, and culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; The white and yellowish colonies were streaked on the MRS solid medium, and purified and cultivated under anaerobic conditions at 37°C. This operation was repeated 3 times to obtain a purified single colony; pick a single colony and place it on the MRS solid medium Mark the upper line, culture anaerobically at 37°C for 36 hours, and perform Gram staining on the obtained colonies (for the Gram staining method, refer to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the morphology of the colonies, and according to the textbook "Common Bacterial System Identification Manual" (Autho...

Embodiment 2

[0100] Embodiment 2: Preparation of Lactobacillus rumen CCFM1141 bacterial suspension

[0101] (1) Dip the bacterial solution of Lactobacillus rumen CCFM1141 from the glycerol tube, streak it on the MRS solid medium, and culture it at 37°C for 48 hours under anaerobic environment to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, In an anaerobic environment, culture at 37° C. for 48 hours for activation culture, and repeat this operation 3 times to obtain the activated bacterial liquid.

[0102] (2) Inoculate the activated bacterial liquid obtained in step (1) into the MRS liquid medium according to the inoculum size of 2% (v / v), cultivate at 37°C for 24 hours to obtain a fermentation liquid, and collect the bacteria by centrifugation of the fermentation liquid body, resuspend the cell body with physiological saline, and adjust the number of viable cells to 5×10 9 CFU / mL, made into bacterial suspension.

Embodiment 3

[0103] Example 3: Relief effect of Lactobacillus rumen CCFM1141 on the symptoms of DSS-induced colitis mice

[0104] The treatment steps for modeling and Lactobacillus rumen CCFM1141 acting on DSS-induced colitis mice are as follows:

[0105] (1) Preparation of 2.5% dextran sodium sulfate (DSS) solution: the DSS was made into 2.5% (w / v) dextran sodium sulfate (DSS) solution with sterilized tap water.

[0106] (2) 24 8-week-old healthy male C57BL / 6J mice were randomly divided into 3 groups, and the 3 groups were respectively named: blank control group (Control), model group (DSS), Lactobacillus rumen CCFM1141 intervention group ( CCFM1141+DSS); 8 mice were used in each group, and the experimental scheme and treatment methods of mice in each group are shown in Table 4.

[0107] (3) Treat the blank control group (Control), the modeling group (DSS), and the Lactobacillus rumen CCFM1141 intervention group;

[0108] Among them, the treatment method of the Lactobacillus rumen CCFM1...

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Abstract

The invention discloses a strain of rumen lactobacillus for relieving colitis and its application, belonging to the field of biotechnology. The invention provides a strain of Lactobacillus rumen CCFM1141, which can have immune regulation ability, can improve intestinal barrier, alleviate colitis, and significantly reduce the disease activity index of mice during DSS-induced colitis. Compared with the model group mice, the tissue damage of the mice in the Lactobacillus rumen CCFM1141 intervention group was significantly reduced, the pro-inflammatory cytokines IL-1β and IL-17 in the colon tissue were significantly reduced, and the concentrations of acetic acid, propionic acid and butyric acid were significantly increased . The medicine prepared by adopting the Lactobacillus rumen CCFM1141 provided by the invention has certain advantages compared with the traditional medicine used for treating colitis, and the strain can be used to make probiotic preparations and the like, and has broad market prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rumen for relieving colitis and its application, in particular to a strain capable of relieving colitis, which can be added to various health foods and health foods, and belongs to the technical field of microorganisms. Background technique [0002] Ulcerative colitis is a diffuse inflammatory response characterized by intracrypt abscesses and infiltration of neutrophils, eosinophils, and plasma cells that attack the colonic lining. The most common symptoms of UC patients are diarrhea, rectal bleeding, tenesmus, mucus discharge, and abdominal pain. [0003] The etiology of ulcerative colitis is still unknown, and it is currently considered to be the result of the interaction between host response caused by foreign substances, gene and immune influence. [0004] Bloody diarrhea is the most common early symptom of ulcerative colitis. Other symptoms include abdominal pain, blood in the stool, weight loss...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L2/38A23L33/135A61K35/747A61P1/04A61P29/00C12R1/225
CPCC12N1/20A23L2/382A23L33/135A61K35/747A61P1/04A61P29/00A23V2002/00A23V2400/177A23V2200/32
Inventor 杨波陈卫王烁崔树茂陆文伟赵建新张灏
Owner JIANGNAN UNIV
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