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Nano-drug for tumor ferroptosis-gas synergistic treatment and preparation method thereof

A nano-drug, ferroptosis technology, applied in nano-drugs, anti-tumor drugs, preparations for in vivo experiments, etc., can solve the problems of high toxicity of manganese ions, unfavorable clinical application, and low loading of nano-drugs, etc. Application prospect, simple operation effect

Active Publication Date: 2021-05-28
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The loading capacity of the nanomedicine synthesized by this method is not high, and the manganese ion itself has high toxicity, which is not conducive to clinical application.

Method used

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  • Nano-drug for tumor ferroptosis-gas synergistic treatment and preparation method thereof
  • Nano-drug for tumor ferroptosis-gas synergistic treatment and preparation method thereof
  • Nano-drug for tumor ferroptosis-gas synergistic treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Preparation of Nanomedicine for Tumor Ferroptosis-Gas Synergistic Therapy

[0070] 1. Synthesis of hollow mesoporous silicone nanoparticles containing tetrasulfide bonds

[0071] Weigh 0.1g of triethanolamine (TEA) and 0.5g of cetyltrimethylammonium chloride (CTAC) into a 50mL flask, add 22mL of water, ultrasonically disperse them evenly, place them in an oil bath at 80°C, and stir ; After the temperature of the system was stabilized, 1.0 mL of tetraethyl silicate (TEOS) was added dropwise thereto as a silicon source, and the reaction was performed for 1 hour to obtain a mesoporous silicon core; (Triethoxymethylsilane) Propyl] Tetrasulfide (BTES) uniform mixed solution, wherein BTES can be used as a silicon source; stop the reaction after continuing the reaction for 3 hours, wash with ethanol and centrifuge several times until the supernatant is transparent color, discard the supernatant, and the resulting precipitate is MSN@MON; disperse the prepared MSN@MON...

Embodiment 2

[0080] Embodiment 2 The loaded carbonyl iron compound is dinonylcarbonyl iron, and the others are the same as in Embodiment 1

[0081] Take 16mg dinonylcarbonyl iron (Fe 2 (CO) 9 ) was dissolved in 20mL tetrahydrofuran, 20mg of HMON-SH was added, stirred in a 70°C oil bath, and the reaction was continued for 2 hours, then the reaction was stopped and centrifuged to obtain the intermediate HMON@Fe loaded with dinonylcarbonyl iron 2 (CO) 9 , for the next reaction.

Embodiment 3

[0082] Example 3 Modification of HMON@FeCO / CDDP with reduced bovine serum albumin and RGD

[0083] Firstly, 40mg / mL bovine serum albumin aqueous solution was reduced by using 1mol sodium borohydride solution, and the reaction was carried out under ice bath conditions, and then 40mg / mL reduced bovine serum albumin aqueous solution was obtained; 5mL HMON-SH@ FeCO / CDDP dispersion, add 2.5mL reduced bovine serum albumin aqueous solution and 10mL deionized water, stir and react at 50°C for 2 hours, centrifuge, discard the supernatant to obtain HMON-SH@FeCO / CDDP-rBSA nanoparticles, Add water to make up to 5mL, set aside.

[0084]Take 5mL HMON-SH@FeCO / CDDP-rBSA solution, add activators N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and then add 100uL cRGD2 for grafting to obtain HMON-SH@FeCO / CDDP-rBSA-RGD2, which is the nanomedicine (HFePtNPs) modified by reduced bovine serum albumin and RGD.

[0085] Analysis results:

[0086] ...

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Abstract

The invention discloses a nano-drug for tumor ferroptosis-gas synergistic treatment. The nano-drug comprises biodegradable hollow mesoporous organic silicon nano particles, carbonyl iron compounds loaded in the hollow mesoporous organic silicon nano particles and a small molecule drug. The invention further discloses a preparation method of the nano-drug for tumor ferroptosis-gas synergistic treatment and application of the nano-drug to preparation of tumor treatment drugs. The preparation method is simple to operate and can be used for repeated large-scale preparation. According to the nano-drug disclosed by the invention, sulfur-sulfur bonds are introduced to react with over-expressed glutathione in a tumor micro-environment, so that a hollow mesoporous organic silicon skeleton is broken, the loaded small-molecule drug is released, and the whole nano-drug is degraded; and the loaded carbonyl iron compound responds to excessive hydrogen peroxide in a tumor micro-environment, so that CO can be released, ferroptosis can be realized, and the synergistic effect of gas treatment and ferroptosis can be realized.

Description

technical field [0001] The invention relates to the technical field of nano-medicines, in particular to a nano-medicine for synergistic treatment of tumor ferroptosis-gas and its preparation method and application. Background technique [0002] At present, the commonly used treatment methods for cancer include surgery, radiotherapy and chemotherapy. Traditional treatment methods often have defects such as high toxicity and side effects, difficulty in precise drug administration, and inevitably cause varying degrees of damage to normal cells in the body, which is not conducive to long-term treatment. treat. With the development of nanotechnology, nanomaterials have attracted attention in the field of medicine. Many researchers have used nanomaterials to accumulate in tumor sites through the EPR effect and good biocompatibility to achieve targeted tumor therapy. Treatment methods such as photodynamic therapy and photothermal therapy have emerged one after another, and the dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/59A61K47/60A61K47/64A61K33/243A61K33/26A61K45/06A61K49/10A61K49/12A61K49/14A61P35/00B82Y5/00
CPCA61K47/6949A61K47/6921A61K47/6901A61K47/643A61K47/64A61K47/60A61K45/06A61K47/593A61K33/26A61K33/243A61P35/00A61K49/10A61K49/126A61K49/143A61K49/14B82Y5/00A61K2300/00
Inventor 沈折玉赵晨阳吴爱国
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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