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Nano-fiber bone tissue engineering scaffold with core-shell-shish-kebab structure and preparation method thereof

A technology of bone tissue engineering and nanofibers, which is applied in the field of biomedical engineering bone repair scaffolds, can solve the problems of polycaprolactone's strong hydrophobicity, poor cell adhesion, and lack of biological activity, and achieve good biocompatibility and degradability Sexuality, remarkable effect, effect of improving hydrophilicity

Pending Publication Date: 2021-06-01
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polycaprolactone is highly hydrophobic, has poor cell attachment, and lacks biological activity, which limits its further applications.

Method used

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  • Nano-fiber bone tissue engineering scaffold with core-shell-shish-kebab structure and preparation method thereof
  • Nano-fiber bone tissue engineering scaffold with core-shell-shish-kebab structure and preparation method thereof
  • Nano-fiber bone tissue engineering scaffold with core-shell-shish-kebab structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Dissolve 12 mg of sodium hyaluronate in 988 microliters of deionized water and mix well to prepare a 1.2 wt% sodium hyaluronate hydrosol. A mixture containing 3.03ml of dichloromethane and 0.01g of Span-80 was mixed with sodium hyaluronate sol and stirred at high speed to obtain a uniform water-in-oil (W / O) emulsion. Dissolve 0.5g of polycaprolactone and 2.11ml of N,N-dimethylformamide in the above emulsion to obtain an electrospinning solution, suck the spinning solution into a syringe for electrospinning, the spinning voltage is 18kv, and spray The distance between the filament head and the receiving plate is 15 cm, and the propulsion speed of the propulsion pump is 0.001 mm / s, and a nanofiber membrane MS with a core-shell structure is prepared.

[0033] figure 1 , figure 2 The scanning electron microscope picture of the scaffold shows a smooth and continuous nanofiber structure, and the diameter of the nanofiber is 127±33nm analyzed by ImageJ software.

Embodiment 2

[0035] Dissolve 0.2 g of polycaprolactone in 20 ml of a mixed solvent of glacial acetic acid and deionized water, and stir magnetically at 60° C., wherein the volume ratio of glacial acetic acid and deionized water is 3:1. After fully dissolving, the solution was cooled to room temperature, and the nanofibrous membrane with core-shell structure obtained in Example 1 was soaked in the dilute polycaprolactone solution for 5 min, 10 min, 15 min, and 20 min, respectively. After vacuum drying for 24 hours, the residual solvent was removed to obtain nanofiber bone tissue engineering scaffolds SK5, SK10, SK15, SK20 with core-shell-kebab structure.

[0036] Figure 3~Figure 6 It is the scanning electron microscope image of core-shell-string structure nanofiber bone tissue engineering scaffolds with different soaking times. It has been determined that the string crystal structure on the surface of core-shell structure nanofibers can improve the hydrophilicity of core-shell structure na...

Embodiment 3

[0038] Dissolve 12 mg of sodium hyaluronate in 988 microliters of deionized water and mix well to prepare a 1.2 wt% sodium hyaluronate hydrosol. Then, mix 10µl Glucosamine (100µg / ml) into 50µl Hyaluronic Acid Hydrosol. A mixture containing 3.03 ml of dichloromethane and 0.01 g of Span-80 was mixed with the above sol and stirred at high speed to obtain a uniform water-in-oil (W / O) type emulsion. Dissolve 0.5g of polycaprolactone and 2.11ml of N,N-dimethylformamide in the above emulsion to obtain an electrospinning solution, suck the spinning solution into a syringe for electrospinning, and the spinning voltage is 18kv, The distance between the spinneret and the receiving plate is 15cm, and the propulsion speed of the propulsion pump is 0.001mm / s, and the nanofibrous membrane MS-GLU ( Figure 8 , Figure 9 shown).

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Abstract

The present invention provides a nano-fiber bone tissue engineering scaffold with the core-shell-shish-kebab structure and the preparation method thereof, the core-shell structure realizes drug loading and slow release functions, and the shish-kebab structure formed by nano-chips growing on the surface of nano-fibers in situ improves the hydrophilicity and cytocompatibility of a nano-fiber membrane with the core-shell structure. The method comprises the following steps: (1) taking glucosamine and sodium hyaluronate hydrosol as a water phase; (2) stirring and mixing dichloromethane, Span80 and the sol at a high speed to obtain a water-in-oil emulsion; (3) dissolving polycaprolactone and N, N-dimethylformamide in the emulsion, and carrying out electrostatic spinning to obtain a nanofiber membrane with a core-shell structure; and (4) soaking the nanofiber membrane with the core-shell structure in a polycaprolactone dilute solution to obtain the nanofiber bone tissue engineering scaffold with the core-shell-shish-kebab structure. The nano-fiber bone tissue engineering scaffold with the core-shell-shish-kebab structure has a certain application value in the biomedical fields of drug loading, slow release, bone repair and the like.

Description

technical field [0001] The invention relates to a nanofibrous bone tissue engineering scaffold with a core-shell-string crystal structure and a preparation method thereof, which belongs to the field of biomedical engineering bone repair scaffolds and has wide application value in effective drug slow release control and bone repair. Background technique [0002] Bone is a complex organ with the functions of hematopoiesis, storage of vital minerals, preservation of vital organs and facilitation of movement. Natural bone consists of approximately 70 wt% inorganic nanocrystals (such as hydroxyapatite) and 30 wt% organic matrix (such as collagen nanofibers). Due to the insufficient number of blood vessels in the bone tissue, which has a high mineral content, bone healing is very difficult in the case of a large amount of tissue loss. Although human bones have the ability to heal and regenerate, this ability is ineffective for injuries caused by large bone defects, fractures, tra...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20A61L27/50A61L27/54A61L27/58D01F8/14D01F8/18
CPCA61L27/18A61L27/20A61L27/50A61L27/54A61L27/58D01F8/14D01F8/18A61L2400/12A61L2430/02A61L2300/602A61L2300/412A61L2300/232
Inventor 胡银春丁慧秀黄棣魏延
Owner TAIYUAN UNIV OF TECH
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