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A kind of preparation method and application of multilayer graphene oxide modified microbial carrier

A microbial carrier and fossil technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of reducing the speed and amount of microbial adhesion, and achieve good purification effect , Improve thermal stability, increase the effect of surface structure

Active Publication Date: 2022-06-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, commonly used microorganism carriers, such as polyurethane carriers, the hydrophobicity of its surface will directly reduce the adhesion speed and amount of microorganisms on its surface.

Method used

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  • A kind of preparation method and application of multilayer graphene oxide modified microbial carrier
  • A kind of preparation method and application of multilayer graphene oxide modified microbial carrier
  • A kind of preparation method and application of multilayer graphene oxide modified microbial carrier

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0051] First aspect, the invention provides a kind of preparation method of multi-layer graphene oxide modified microorganism carrier, such as figure 1 As shown, the method specifically includes the following steps:

[0052] In step 1 (S11), the first post-treated polyurethane carrier is irradiated with an ultraviolet lamp to obtain a first intermediate product.

[0053] In this embodiment, the first post-treated polyurethane carrier has the characteristics of being clean and dry. In the specific implementation, the clean and dry polyurethane carrier is laid flat under the ultraviolet lamp for 30-60 minutes. During the irradiation of the ultraviolet lamp, the amide bond in the polyurethane is broken and the carboxylic acid group is formed to obtain the first intermediate product.

[0054] Optionally, in step 1 of this embodiment, the first post-processing includes:

[0055] Put the polyurethane sponge in an ultrasonic cleaner to shake and wash for 30 to 40 minutes, and then ...

Embodiment 1

[0070] Embodiment 1 (the preparation of multilayer graphene oxide modified microorganism carrier)

[0071] (1) Cut the polyurethane carrier into a sphere with a diameter of about 1 cm, put it into an ultrasonic cleaner for oscillating washing for 30min, wash with deionized water, and place it in an oven to dry to obtain a clean polyurethane carrier;

[0072] (2) laying the clean and dry polyurethane carrier under the ultraviolet lamp for 60 minutes, the amide bond in the polyurethane is broken to form a carboxylic acid group, and the first intermediate product is obtained;

[0073] (3) configure an ethanol solution of 6wt% silane coupling agent KH550 (γ-aminopropyltriethoxysilane, APTES), and ultrasonically vibrate for 10min to fully disperse the solution;

[0074] (4) Preparation of graphene oxide: add 1.5g graphite powder to 200mL of a 9:1 sulfuric acid / phosphoric acid mixed solution, stir evenly, and place in an ice-water bath; slowly add 9.0g potassium permanganate, stir t...

Embodiment 2

[0079] Example 2 (immobilization of microorganisms)

[0080] Using the graphene oxide modified polyurethane carrier prepared in Example 1, a biofilm formation test was carried out. The biological immobilization carrier was first soaked with the nutrient solution, placed in a 500mL glass conical flask, and 200mL of the nutrient solution was added to the bottle. Inoculate the high-efficiency microbial preparation with independent intellectual property rights of Peking University at 4g / L. Incubate in a constant temperature shaker at a temperature of 30°C.

[0081] On day 21 of culture, Figure 5 The SEM image of the unmodified polyurethane carrier for the hanging film culture test is shown, Image 6 The SEM image of the multi-layer graphene oxide-modified microbial carrier prepared in Example 1 after the hanging film culture test is shown. like Figure 5 and 6 As shown, compared with the unmodified polyurethane carrier control, on the 21st day of culture, the amount of film...

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Abstract

The invention provides a preparation method and application of a multilayer graphene oxide modified microbial carrier. The method uses silane coupling agent to modify the surface function of the polyurethane carrier, and by grafting multi-layer graphene oxide on the surface of the carrier, the specific surface area and surface structure of the carrier are increased, and the surface attachment process of microorganisms is accelerated, thereby improving the biological properties of the carrier. Loading capacity; and, introducing hydrophilic functional groups into the polyurethane carrier through graphene oxide, improving the hydrophilicity of the modified polyurethane bio-carrier, thereby enhancing the bio-affinity of the carrier. At the same time, the preparation method of the multilayer graphene oxide modified microbial carrier provided by the present invention has the characteristics of simple process, low cost, and strong thermal stability of the modified carrier. The modified microbial carrier prepared by it will help to solve the problem of biological immobilization The problem of long film-hanging time in the process has a good application prospect in the biological reaction sewage treatment system.

Description

technical field [0001] The invention relates to the field of sewage treatment, and mainly relates to a preparation method and application of a multi-layer graphene oxide modified microbial carrier. Background technique [0002] With the continuous increase of sewage and wastewater discharge, more and more attention has been paid to water environment problems. The most commonly used sewage biological treatment methods are activated sludge method and biofilm method. Among them, the biofilm method uses the surface of the carrier as a medium to locate the microbial cells in the free state on the surface of the carrier to form a biofilm dominated by microorganisms, so that it can maintain its activity and can be reused repeatedly. Significant advantages such as strong impact resistance and low power consumption. [0003] The microbial carrier is a key factor in the sewage biofilm treatment process and plays a vital role in the treatment effect of the biological reaction. Its ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C08J3/28C08L75/04
CPCC02F3/34C08J3/28C08J2375/04
Inventor 籍国东王亚芹
Owner PEKING UNIV
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