A kind of Micromonospora tmd166 and its application

A technology of TMD166, Micromonospora, applied in the fields of microbiology and pharmacy, to achieve the effect of strong inhibitory activity and good development prospects

Active Publication Date: 2022-02-15
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the antibacterial activity of thiopeptide antibiotics mainly prevents the synthesis of bacterial proteins through bacterial ribosomes, different types of thiopeptide antibiotics have different targets, and the discovery of new structures of thiopeptide antibiotics is very likely to develop novel therapeutic drugs. Tackling human and animal-associated infections and bacterial resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Micromonospora tmd166 and its application
  • A kind of Micromonospora tmd166 and its application
  • A kind of Micromonospora tmd166 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The isolation of embodiment 1 Micromonospora bacterial strain TMD166

[0049] The camel thorns collected from the Taklamakan Desert were washed with water, and the surface impurities were removed by ultrasonic waves. The samples were soaked in 1% Tween-20 for 1 minute, sodium hypochlorite aqueous solution containing 0.4% available chlorine for 7 minutes, and 2.5% sodium thiosulfate. Soak for 9 minutes, soak in 75% ethanol for 2 minutes, soak in 10% sodium bicarbonate for 10 minutes, wash with sterile water 3 times after each soak. After the sample was dried, it was ground into powder with a pulverizer and evenly sprinkled on the M-WA medium (glycerol 10g, yeast 0.5g, proline 1.0g, aspartic acid 1.0g, potassium nitrate 0.5g, pyruvate Sodium 1.25g, betaine 1.25g, agar 20g, sterile water 1L, pH 8.0, sterilized at 121°C for 30 minutes), incubated at 28°C. At the same time, after surface disinfection, 200 μl of distilled water for the third wash was taken, spread on the ISP...

Embodiment 2

[0050] Identification of Example 2 Micromonospora bacterial strain TMD166

[0051] (1) Morphological identification:

[0052] Micromonospora TMD166 of the present invention, such as figure 1 shown. The colony on the ISP-2 medium shows a light orange color, and the surface is covered with a layer of powdery spores, and grows rapidly at a culture temperature of 28-30°C.

[0053] (2) Molecular identification:

[0054] The genomic DNA was extracted, amplified and analyzed using universal primers 27f and 1492r, and a single DNA band was obtained. In the GenBank database, carry out Blast comparison, screen the typical strain sequence with the highest similarity, then use MEGA5.2 software to carry out cluster analysis and construct phylogenetic tree with neighbor method (Neighbor-joining), such as figure 2 As shown, TMD166 is associated with Micromonospora polyrhachis NEAU-ycm2 T Gathered in the same branch, with a similarity of 99.48%, the endophyte TMD166 was finally identifi...

Embodiment 3

[0055] The fermentation of embodiment 3 Micromonospora bacterial strain TMD166

[0056] Micromonospora strain TMD166 grown in the slant medium was inoculated on the seed medium, and the seed medium was No. 38 medium (glucose 4.0g, yeast powder 4.0g, malt extract powder 5.0g, ZnSO 4 .7H 2 O 0.001g, MnCl 2 .4H 2 O0.001g, FeSO 4 .7H 2 O 0.002g, phenylalanine 0.001g, alanine 0.0003g, vitamin B1 0.001g, vitamin B20.001g, vitamin B6 0.001g, niacin 0.001g, biotin 0.001g, 1.0L sterile distilled water, pH 8.5 ). Put 100mL No. 38 medium in each 500mL shake flask, culture on a 28-30°C, 180rpm rotary shaker for 72h, and then transfer to the fermentation medium (same as the seed medium) with 10% inoculum amount, and put in each 5.0L shake flask Pack 1.0L of fermentation medium, culture at 28-30°C on a rotary shaker at 180rpm for 96h, and harvest the fermentation broth.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the fields of microbiology and pharmacy, and discloses Micromonospora TMD166 and its application, more specifically, a preparation method and application of Micromonospora TMD166 and a novel thiopeptide antibiotic produced therefrom. The Micromonospora sp. TMD166 of the present invention has a preservation number of CGMCC NO.21622. Micromonospora (Micromonospora sp.) of the present invention contains a novel thiopeptide antibiotic—Xuanzangmicin (Xuanzangmicin) in the fermented liquid, and this compound has good antibacterial activity, as a new human or animal Anti-infective drugs have a good application prospect.

Description

technical field [0001] The invention relates to the fields of microbiology and pharmacy, in particular to a preparation method and application of Micromonospora TMD166 and a novel thiopeptide antibiotic produced therefrom. Background technique [0002] Pathogens represented by "ESKAPE" (Enterococcus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) and Mycobacterium tuberculosis and their antibiotic resistance Bacteria, including multidrug-resistant or pan-drug-resistant bacteria, seriously threaten the health of humans and animals. At present, with the resistance of these pathogenic bacteria to therapeutic antibacterial drugs, the effective and available antibiotics are gradually decreasing. Therefore, antibiotics with novel structures and unique mechanisms of action have been discovered, especially the discovery of drug-resistant bacteria against "ESKAPE" and Mycobacterium tuberculosis Effective new comp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C07K9/00C12P21/02A61K38/14A61P31/04C12R1/29
CPCC12N1/20C07K9/006C12P21/02C12P21/005A61P31/04
Inventor 孙承航蒋忠科刘佳萌邬刚刘少伟游雪甫蒙建州
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap