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Dual-response type virus molecularly imprinted sensor based on magnetism and photons, and construction method thereof

A dual-response, molecularly imprinted technology, applied in the field of analytical chemistry, can solve problems such as virus-imprinted polymers, and achieve fast binding kinetics, high adsorption capacity, and simple preparation process

Active Publication Date: 2021-07-06
FOSHAN GREEN INTELLIGENT MFG RES INST OF XIANGTAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no technical reports on magnetically and photonic dual-stimuli-responsive virus-imprinted polymers.

Method used

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  • Dual-response type virus molecularly imprinted sensor based on magnetism and photons, and construction method thereof
  • Dual-response type virus molecularly imprinted sensor based on magnetism and photons, and construction method thereof
  • Dual-response type virus molecularly imprinted sensor based on magnetism and photons, and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0129] A novel method for the construction of a dual-response virus molecularly imprinted sensor based on magnetism and photonics, such as figure 1 shown, including the following steps:

[0130] (1) Fe 3 o 4 @SiO 2 Preparation of nanoparticles: 100 mL of isopropanol and 4 mL of ultrapure water (isopropanol: ultrapure water = 100:2~8) mixture was used as a solvent to prepare Fe 3 o 4 @SiO 2 Nanoparticles. First, 500 mg Fe 3 o 4 Nanoparticles (about 100-200 nm) were added to the mixture, and placed in an ultrasonic oscillator for ultrasonic treatment and dispersion for 20 minutes. Under mechanical stirring, 5 mL NH 3 ·H 2 O was added to the above mixture, and then 2 mL of tetraethyl orthosilicate (TEOS) was slowly added at a controlled rate (20 drops / min), and the reaction solution was placed at 45 °C for 6 hours. After the reaction was completed, the particles were collected with a magnet, washed several times with deionized water and ethanol in turn, and dried in a ...

Embodiment 2

[0138] Example 2: Characterization of the performance, morphology and structure of the MMIPs resonant photosensor and intermediate products

[0139] The structure and morphology of all the materials prepared in Example 1 were characterized by Fourier transform infrared spectrometer, X-ray diffractometer and scanning electron microscope. figure 2 for Fe 3 o 4 Particle (a), Fe 3 o 4 @SiO 2 -COOH particles (b), Fe 3 o 4 @SiO 2 @UiO-66-NH 2 Particle (c), Fe 3 o 4 @SiO 2 @UiO-66-NH 2 Infrared spectra of -C=C particles (d), MMIPs (e) and MNIPs (f) particles. about 582 cm -1 The absorption peak at is attributed to Fe 3 o 4 The characteristic peak of Fe-O; 1095 cm -1 The absorption peak at is the stretching vibration peak of Si-O-Si; 1635 cm -1 The peak at is attributed to the vibrational absorption of the C=O bond in the amide, indicating that APTES was successfully coated on the particle and modified -COOH from succinic anhydride; continued to modify UiO-66-NH 2 ...

Embodiment 3

[0142] Example 3: Application of the resonant optical molecular imprinting sensor

[0143] (1) The experimental conditions of this example are: the dosage of MMIPs is 90 ng / mL, the pH is 7.4, the adsorption time is 20 min, and the temperature is 25°C. The specific implementation is: take 5 U / mL EV71 and MMIPs in 90 ng / mL Tris-HCl buffer, adjust the pH of the whole system to 7.4, and measure the resonance light intensity after shaking and adsorbing at 25 °C for 20 min ; Then irradiate under 365nm ultraviolet light for 20 min, then measure the resonance light intensity, and then measure the resonance light intensity after 20 min irradiation under visible light.

[0144] (2) Detection and analysis of different concentrations of EV71 by MMIPs resonant light sensor

[0145] Under optimized conditions, the prepared MMIPs and MNIPs were used to detect and analyze different concentrations of EV71. Such as Figure 5 As shown in A, after adding different concentrations of EV71, the r...

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Abstract

The invention discloses a dual-response type virus molecularly imprinted sensor based on magnetism and photons, and a construction method thereof. The dual-response type virus molecularly imprinted sensor based on magnetism and photons takes magnetic particles as an inner core, the surfaces of the magnetic particles are coated with silicon dioxide, a metal organic framework is grafted to the silicon dioxide, the metal organic framework is connected with a photoresponse polymer, and the photoresponse polymer is connected with a target virus template. The dual-response type virus molecular imprinting sensor based on magnetism and photons has a good binding behavior on a template virus, and is fast in binding kinetics, high in adsorption capacity and good in selectivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and mainly relates to a dual-response virus molecular imprint sensor based on magnetism and photons and a construction method thereof. Background technique [0002] Over the past few decades, molecularly imprinted polymers (MIPs), a type of "artificial antibody", have shown great potential in various fields, especially in health and life sciences. Using molecular imprinting technology, new artificial analog binding sites are constructed, which are complementary to the chemical structure and spatial structure of template molecules. Molecularly imprinted polymers have good selectivity, excellent stability and low preparation cost. So far, small molecule imprinting technology has achieved great success, however, under the influence of factors such as size, structural complexity and conformational flexibility, molecularly imprinted polymers for biological macromolecules (such as proteins, viruses,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00B01J20/22B01J20/26B01J20/28B01J20/30G01N27/72G01N21/01G01N21/84
CPCC08G83/001B01J20/06B01J20/226B01J20/268B01J20/103B01J20/28009G01N27/72G01N21/01G01N21/84
Inventor 蔡昌群唐丽梁琨淞龚行
Owner FOSHAN GREEN INTELLIGENT MFG RES INST OF XIANGTAN UNIV
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