A kind of hydrogen peroxide fluorescent probe and its preparation method and application
A fluorescent probe and reaction technology, applied in chemical instruments and methods, fluorescence/phosphorescence, material analysis through optical means, etc., can solve the problems of insufficient selectivity and interference of hydrogen peroxide, and achieve good application prospects, high Effect of selectivity and light property stabilization
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[0039] The present invention provides a method for preparing the fluorescent probe described in the above technical solution, comprising the following steps:
[0040] Mixing the first compound of the structure shown in formula II, hydrogenation salt and anhydrous organic solvent to carry out the first substitution reaction; mixing the reaction solution obtained by the first substitution reaction with the second compound of the structure shown in formula III The second substitution reaction obtains the fluorescent probe of the structure shown in formula I;
[0041]
[0042] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.
[0043]In the present invention, the first compound of the structure shown in the formula II is preferably prepared by the following method: under a protective atmosphere, 2,4-dihydroxybenzaldehyde, 2-cyclohexen-1-one and mixed The solvents are mix...
Embodiment 1
[0064] Under nitrogen protection, dissolve 4mmol 2,4-dihydroxybenzaldehyde and 4mmol 2-cyclohexen-1-one in 12mL tetrahydrofuran aqueous solution (water: tetrahydrofuran volume ratio = 1:1), at room temperature under stirring conditions After 3 days of substitution reaction, 12 mL of dilute hydrochloric acid with a concentration of 1 mol / L was added to quench the reaction, and the resulting reaction solution was extracted 3 times with ethyl acetate, and the volume of ethyl acetate added each time was 50 mL, and the organic phases were combined and dried over anhydrous sodium sulfate , filtered, the resulting filtrate was distilled under reduced pressure and then separated and purified by gel chromatography column to obtain the first compound (19% yield) of the structure shown in formula II, wherein the eluent used for the separation and purification of the gel chromatography column was A volume ratio of 6:1 petroleum ether-ethyl acetate mixed solvent;
[0065]Put 4mmol of 4-(hy...
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