A multi-bacteria fusion symbiotic fermentation process for disease resistance and growth promotion
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A symbiotic fermentation and process technology, which is applied in the field of multi-bacteria fusion symbiotic fermentation process, can solve the problems of high process cost and single colony, and achieve the effects of increasing dissolved oxygen, diversifying bacterial flora, and shortening the treatment process
Active Publication Date: 2022-01-11
黎昊源生物工程(山东)股份有限公司
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[0006] In order to solve the problems of single bacterial colony and high process cost produced by the current fermentation process, this application provides a multi-bacteria fusion symbiotic fermentation process for disease resistance and growth promotion
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Embodiment 1
[0054] A multi-bacteria fusion symbiotic fermentation process for disease resistance and growth promotion, comprising the following steps:
[0055] Step (1-1), configuration culture medium:
[0056] Configure medium A: Weigh 3g beef extract, 10g peptone, 5g sodium chloride and 20g agar, add to distilled water, mix well, and add distilled water to make the system 1000mL, adjust the pH value to 7.2, and then in the temperature of 121℃ , and sterilized for 30 minutes under the condition of a pressure of 0.10 MPa to prepare medium A.
[0057] Prepare medium B: Weigh 200g of potato filtrate, 20g of glucose and 20g of agar, add them to distilled water, mix well, and add distilled water to make the system 1000mL, then sterilize for 30min at a temperature of 121°C and a pressure of 0.10MPa , to prepare medium B.
[0065] The difference between this embodiment and the above-mentioned embodiment 1 lies in: the condition parameter changes in the fusion symbiotic fermentation process in step (2).
Embodiment 3
[0067] The difference between this embodiment and the above-mentioned embodiment 1 lies in: the condition parameter changes in the fusion symbiotic fermentation process in step (2).
[0068] The conditional parameters in above-mentioned embodiment 1-3 are as shown in table 1 below:
[0069] The condition parameter table of multi-bacteria fusion symbiotic fermentation in the embodiment 1-3 of table 1
[0070]
[0071]
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Abstract
This application relates to the field of microbial fermentation, more specifically, it relates to a multi-bacteria fusion symbiotic fermentation process for disease resistance and growth promotion, including the following steps: Step (1), preparing bacterial liquid: preparing Paenibacillus polymyxa respectively liquid, yeast liquid, Streptococcus thermophilus liquid; step (2), fusion symbiotic fermentation: add fermentation medium to the fermentation equipment, control the pH value and temperature of the fermentation medium, and add Paenoid polymyxa while stirring the fermentation medium Bacillus liquid, continue to stir and ferment; then control the pH value and temperature of the system, add yeast liquid while stirring, continue to stir and ferment; then control the pH value and temperature of the system, add Streptococcus thermophilus liquid while stirring, continue to stir and ferment, and produce Multi-bacteria fusion symbiotic fermentation broth. The multi-bacteria fusion symbiotic fermentation process of the present application mixes growth-promoting rhizosphere probiotics and disease-resistant rhizosphere probiotics through mixed fermentation, reducing the production cost of bacterial agents, with diverse flora, strong adaptability to the environment, and a wide range of applications .
Description
technical field [0001] This application relates to the field of microbial fermentation, more specifically, it relates to a multi-bacteria fusion symbiotic fermentation process for disease resistance and growth promotion. Background technique [0002] In natural ecosystems, different microorganisms restrict and balance each other in terms of living space and nutrient requirements. In the soil environment, artificial fertilization and drug intervention destroy the original microbial species and quantity, break the balance, and increase the biomass of pathogenic bacteria in crops, which leads to the occurrence of soil-borne diseases and bacterial and fungal diseases, and then damages plants. impact on growth, yield and quality. [0003] In the current technology, artificially adding beneficial microorganisms in the rhizosphere of plants can change the microbial community system, inhibit pathogenic bacteria, and promote the growth and resistance of plants. [0004] At present,...
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