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Multifunctional anti-tumor nano-drug as well as preparation method and application thereof

A nano drug, anti-tumor technology, applied in the field of biomedicine

Active Publication Date: 2021-08-17
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although NIR-II fluorescence imaging has so many advantages, there is no way to effectively provide more comprehensive tumor information only through this imaging method

Method used

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  • Multifunctional anti-tumor nano-drug as well as preparation method and application thereof
  • Multifunctional anti-tumor nano-drug as well as preparation method and application thereof
  • Multifunctional anti-tumor nano-drug as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of amino-modified mesoporous Ag 2 S@SiO 2 core-shell nanoparticles

[0034] Using silicon dioxide (SiO 2 ) as carrier particles, silver sulfide quantum dots (Ag 2 S QDs) are used as near-infrared second-region fluorescent reagents and photothermal reagents, and n-tetradecyl alcohol is used as a phase change material for coating. Such as figure 1 and figure 2 As shown, the following is the amino-modified mesoporous Ag 2 S@SiO 2 Specific preparation method of core-shell nanoparticles: Synthesis of Ag using thermal decomposition by existing methods 2 SQDs and thiol-capped quantum dots were dispersed in chloroform and stored in a 4°C refrigerator for later use. Dissolve 0.05 g of CTAB (cetyltriethylammonium bromide) in 2.5 mL of deionized water, then add 0.25 mL of Ag dispersed in chloroform 2 In S QDs, stir vigorously for 30 min to form a brown-yellow sol; put the sol in a 60°C water bath and stir for 10 min to evaporate chloroform; then add...

Embodiment 2

[0035] Example 2 Preparation of multifunctional anti-tumor nano-drugs

[0036] Step 1. Amino-modified mesoporous Ag 2 S@SiO 2 For the formation of core-shell nanoparticles, refer to Example 1.

[0037] Step 2. Folic acid (FA) activation: Dissolve 2.4 mg FA in 10 mL PBS solution (pH 5.6). Then 5 mg / 3 mg of EDS / NHS (carbodiimide / N-hydroxysuccinimide) was added to the above solution to obtain the activated FA solution.

[0038] Step 3, FA modification of AS nanoparticles: After the activated FA solution was reacted at room temperature for 30 minutes, the pH value of the FA solution was adjusted to 7.4 with NaOH, and 12 mg AS NPs were added to the FA solution. After stirring at room temperature for 5 h, the mixture was centrifuged to obtain folate-conjugated ASF NPs (i.e., AS-FA NPs). AS nanoparticles are targeted by surface modification of folic acid.

[0039] Step 4. Preparation of DHA and SNP-loaded AS-FA nanoparticles: Take 2 mg AS-FA NPs and disperse them in 2 mL ethanol...

Embodiment 3

[0041] Example 3 Application of multifunctional anti-tumor nano-drugs

[0042] The multifunctional Ag prepared by embodiment 2 2 S@SiO 2 -DHA / SNP / TD diagnostic and therapeutic probes can be used for photothermal / chemotherapy / gas therapy of tumors under near-infrared light irradiation on the one hand, and can be used for fluorescence and photoacoustic imaging in the second near-infrared region on the other hand.

[0043] The following experiment is used to evaluate the combined therapeutic effect of artemisinin derivatives / sodium nitroprusside.

[0044] 1. Photothermal performance testing

[0045] To evaluate the photothermal effect, use solutions of different concentrations of AS NPs to test the photothermal effect, put the AS NPs solution at 808 nm laser, 1W / cm 2 irradiated for 10 minutes. Such as image 3 As shown, the photothermal effect of AS NPs showed obvious concentration dependence, and the temperature did not change significantly within 10 min without AS, while t...

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Abstract

The invention provides a multifunctional anti-tumor nano-drug. The synergistic effect of an artemisinin derivative and sodium nitroprusside serves as the core content of anti-tumor treatment. The nanoparticles are used as a carrier, a fluorescent / photo-thermal material, an artemisinin derivative and sodium nitroprusside are loaded or coated, and a thermal phase change material is coated outside the carrier. In a tumor environment, sodium nitroprusside reacts with glutathione overexpressed in tumor cells to generate nitric oxide gas, so that gas therapy is realized. When nitric oxide is generated, sodium nitroprusside also can generate a large amount of low-valent iron ions, so that peroxy bridged bonds of the artemisinin derivatives are triggered to break, a large amount of active oxygen is generated, and tumor cells are effectively killed. The raw materials are wide in source, the preparation process is simple and easy to operate, and the prepared nanoparticles loaded with the artemisinin derivatives and the sodium nitroprusside have good water solubility, dispersity and biocompatibility and are an ideal multifunctional cancer treatment reagent.

Description

technical field [0001] The present invention relates to a multifunctional anti-tumor nano-medicine and its preparation method and application, in particular to a nano-medicine of artemisinin derivatives and sodium nitroprusside released in temperature-sensitive response guided by near-infrared two-region fluorescence imaging and its preparation method and application. The application of photothermal, gas therapy reagent, near-infrared second region fluorescence and photoacoustic contrast agent in tumor diagnosis and treatment belongs to the field of biomedical technology. Background technique [0002] In recent years, with the continuous development of nanotechnology, it is particularly important to precisely control the synthesis of nanomaterials with special optical properties and functions. Through precise control, low doses of anticancer drugs, greatly improved therapeutic effects, and multiple combinations of therapeutic methods can be achieved, thereby Reversing the re...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/51A61K47/04A61P35/00A61K33/26A61K31/357A61K49/22A61K49/00
CPCA61K41/0052A61K9/5115A61P35/00A61K33/26A61K31/357A61K49/22A61K49/0019A61K49/0002A61K49/0093A61K49/225A61K49/005A61K2300/00
Inventor 沈清明孙志权杜文宇倪海洋范曲立
Owner NANJING UNIV OF POSTS & TELECOMM
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