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Gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material, sensing electrode and preparation method and application of gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material

A technology of graphene quantum dots and nanocomposites, which is applied in the direction of material electrochemical variables, material analysis, and material analysis through electromagnetic means, which can solve the problem of poor anti-interference ability of sensing detection technology and high potential of analyte electrocatalysis , low conductivity of active materials and other issues, to achieve good conductivity, facilitate ion transmission, and increase the effect of reaction rate

Pending Publication Date: 2021-09-03
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The electrochemical sensing and detection technologies of a variety of nanomaterial-based enzyme mimics have been reported so far. Due to the low conductivity of the active material, the electrocatalysis of the analyte requires a relatively high potential.
Under high potential, some electroactive substances coexisting in biological fluids can also be oxidized or reduced at the same time, resulting in poor anti-interference ability and high false positive of sensing detection technology.

Method used

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  • Gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material, sensing electrode and preparation method and application of gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material
  • Gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material, sensing electrode and preparation method and application of gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material
  • Gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material, sensing electrode and preparation method and application of gold nanoparticle/nitrogen-doped graphene quantum dot nano composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation method and performance analysis of gold nanoparticles / nitrogen-doped graphene quantum dot nanocomposite material

[0046] 1. Preparation of nitrogen-doped graphene quantum dots:

[0047] Add 4 mM citric acid and 12 mM urea into 20 mL of ultrapure water, stir well to dissolve them, then transfer to an 80 mL polytetrafluoroethylene-lined autoclave, and heat at 160 °C for 4 hours. After cooling to room temperature, the obtained product was dispersed in 100 mL of ultrapure water and centrifuged at 10 000 rpm for 10 min to remove large quantum dots. Then, the resulting solution was dialyzed against ultrapure water with a 500 Da dialysis bag for 24 hours (the dialysate was changed every 8 hours) to remove unreacted chemicals. Finally, dry at -40°C for 48 hours in a freeze dryer to obtain nitrogen-doped graphene quantum dots.

[0048] The microscopic morphology of nitrogen-doped graphene quantum dots was characterized by TEM analysis test, and the resul...

Embodiment 2

[0054] The difference from Example 1 is that the amount of nitrogen-doped graphene quantum dots was weighed to be 10 mg, and the prepared gold nanoparticles / nitrogen-doped graphene quantum dot nanocomposite material.

[0055] Nitrogen-doped graphene quantum dots and gold nanoparticles / nitrogen-doped graphene quantum dot nanocomposites were characterized by high-resolution transmission electron microscopy (HRTEM) analysis. The results showed that nitrogen-doped graphene quantum dots have high Crystallinity, the lattice spacing is 0.20nm, corresponding to the lattice fringes of graphene (002 crystal plane) ( Figure 4 a) The surface of gold nanoparticles / N-doped graphene QD nanocomposites is mainly composed of exposed Au(111) with a lattice parameter of 0.23 nm, except for lattice fringes from N-doped graphene QDs. surface composition ( Figure 4 b).

[0056] Using TMB-H 2 o 2 The peroxidase-like activity of gold nanoparticles / nitrogen-doped graphene quantum dot nanocomposit...

Embodiment 3

[0058] Preparation method of sensing electrode and analysis of its detection performance

[0059] 1. Preparation of sensing electrodes based on gold nanoparticles / nitrogen-doped graphene quantum dot nanocomposites:

[0060] The disc glassy carbon electrode with a diameter of 3mm is polished on the polishing cloth with alumina with a particle size of 1.0, 0.3 and 0.05μm in turn for 2 minutes, and the surface of the electrode must be rinsed with ultrapure water after each particle size of alumina polishing powder is used. And ultrasound for 5min. Then dry under nitrogen condition to obtain a polished glassy carbon electrode.

[0061] Disperse the gold nanoparticles / nitrogen-doped graphene quantum dot nanocomposite material prepared in Example 2 in ultrapure water, and prepare a 1mg / mL nanocomposite modification layer solution; The material modification layer solution was drop-coated on the surface of the glassy carbon electrode, and dried under a 40W infrared lamp for 40 minut...

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Abstract

The invention discloses a gold nanoparticle / nitrogen-doped graphene quantum dot nano composite material, a sensing electrode and a preparation method and application of the gold nanoparticle / nitrogen-doped graphene quantum dot nano composite material. The method comprises the following steps: a, dissolving citric acid and urea in water to react, cooling to room temperature after the reaction is finished, dialyzing to remove unreacted chemical substances, freeze-drying to obtain nitrogen-doped graphene quantum dots, and dissolving the nitrogen-doped graphene quantum dots in water to obtain a nitrogen-doped graphene quantum dot solution; b, heating and refluxing the nitrogen-doped graphene quantum dot solution, dropwise adding a HAuCl4 solution for reaction, performing cooling to room temperature after the reaction is finished, and performing centrifuging to obtain the gold nanoparticle / nitrogen-doped graphene quantum dot nano composite material. The nano composite material is modified on the surface of an electrode carrier to obtain a sensing electrode, so that the sensing electrode has good stability and biocompatibility, and has higher sensitivity and wider linear range on H2O2 detection; the glucose oxidase is further modified on the surface of the sensing electrode to obtain the biosensing electrode, and the biosensing electrode has excellent selectivity and sensitivity on glucose detection.

Description

Technical field: [0001] The invention belongs to the technical field of electrochemical analysis and detection, and specifically relates to a gold nanoparticle / nitrogen-doped graphene quantum dot nanocomposite material, a gold nanoparticle nitrogen-doped graphene quantum dot nanocomposite material, a sensing electrode and a preparation method thereof and application. Background technique: [0002] Due to its strong oxidizing and reducing properties, hydrogen peroxide (H 2 o 2 ) is widely used as bleaching agent, bactericide and preservative in chemical industry, food processing industry and pharmaceutical field. In addition, H 2 o 2 As one of the main reactive oxygen species, it also plays a vital role in biological systems. However, excess H 2 o 2 Can interfere with the synthesis of DNA genetic molecules and cause neuropsychiatric disorders. Monitor H under the above physiological and chemical conditions 2 o 2 It is of great significance to prevent human tissue da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/30G01N27/3278
Inventor 张友雄吴清平古其会张菊梅孙铭韦献虎丁郁薛亮陈谋通王涓庞锐杨小娟雷涛张淑红
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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