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Micro-ecological preparation for treating necrotic enteritis of broiler chicken and application of micro-ecological preparation

A probiotic and necrotizing enteritis technology, which is applied in the field of probiotics for the treatment of necrotizing enteritis in broilers, can solve the problems of clinical necrotizing enteritis outbreak, reducing drug efficacy, increasing and other problems

Pending Publication Date: 2021-09-14
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long-term and repeated use of antibacterial drugs has led to the emergence of multi-drug resistant bacteria or "super bacteria", which not only reduces the efficacy of drugs, but also causes drug residues in livestock and poultry, affecting food safety
In 2020, my country will completely ban the addition of antibiotics in feed. However, the reduction in the use of antibiotics in feed may increase the risk of clinical necrotic enteritis outbreaks. At present, there is still a lack of effective treatment methods that do not use antibiotics

Method used

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  • Micro-ecological preparation for treating necrotic enteritis of broiler chicken and application of micro-ecological preparation
  • Micro-ecological preparation for treating necrotic enteritis of broiler chicken and application of micro-ecological preparation
  • Micro-ecological preparation for treating necrotic enteritis of broiler chicken and application of micro-ecological preparation

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Preparation of culture medium

[0030] (1) Seed medium: tryptone 8-10 grams, yeast extract 8-10 grams, NaCl 10-12 grams, distilled water 1000 ml, pH value 6.8-7.2, sterilized at 121°C for 20-30 minutes;

[0031] (2) Fermentation medium: corn steep liquor dry powder 25-35 grams, NaCl 10-12 grams, ammonium sulfate 2.5-5 grams, arginine 0.5-1.5 grams, distilled water 1000 ml, pH value 6.6-7.0, sterilized at 121°C 20-30 minutes.

[0032] 2. Preparation of metabolites of Bacillus subtilis S2

[0033] The concrete steps of the preparation of Bacillus subtilis S2 metabolite are as follows:

[0034] (1) After culturing the Bacillus subtilis S2 strain on LB solid medium for 12-14 hours, inoculate it in 100mL seed medium, and cultivate it at 37°C for 12-14 hours to the logarithmic phase;

[0035] (2) Get the logarithmic phase strain seed solution and inoculate it in the fermentation medium with an inoculum size of 4-8%, and shake it for 36-48 hours at 37°C and 200rpm. After...

Embodiment 2

[0040] A single factor design scheme was adopted: 300 1-day-old white-feathered broiler chickens were selected for the test, and the pre-test was carried out for 7 days. Chickens with large weight differences were eliminated, and the remaining 240 broiler chickens officially started the test. The experiment was divided into 4 treatment groups, each treatment group had 3 repetitions (i.e. 3 pens, each pen 1.5m×1.5m), each repeated 20 chickens, half male and half female, negative control group and Do not add any probiotics in the drinking water of positive control group and diet, positive+antibiotic group and positive+test group add virginiamycin (22mg / kg feed) and probiotics (500mg / kg feed). When fed to the age of 21 days, the positive control group, the positive + antibiotic group and the positive + test group were orally administered Clostridium perfringens type A (1.0 mL / bird / day, 1.0×10 8 cfu / mL), the test was completed on the 28th day of age and the corresponding detectio...

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Abstract

The invention provides a micro-ecological preparation for treating necrotic enteritis of broiler chicken and application of the micro-ecological preparation. The micro-ecological preparation is prepared by compounding a bacillus subtilis metabolite and chenodeoxycholic acid according to a weight ratio of (8-10): 1, wherein the bacillus subtilis metabolite is prepared by fermenting and drying bacillus subtilis S2 with a preservation number of CGMCC No. 19824. The micro-ecological preparation can be used for effectively treating the necrotic enteritis of white-feather broiler chicken, improving the production performance of the white-feather broiler chicken, increasing the height of jejunum villi and the depth of crypts, reducing the level of inflammatory factors entering blood circulation, further reducing inflammation caused by the necrotic enteritis and promoting and improving the health level of animal bodies; the using effect of the micro-ecological preparation is not different from that of common antibiotics; AND the micro-ecological preparation can be used for replacing the antibiotics in the treatment of the necrotic enteritis of the white-feather broiler chicken.

Description

technical field [0001] The invention belongs to the technical field of animal feed, and relates to a microecological preparation for treating broiler necrotic enteritis and an application thereof. Background technique [0002] Necrotic enteritis induced by Eimeria maxima and Clostridium perfringens is a multifactorial disease, and the clinical symptoms of acute necrotic enteritis include watery bloody stool (black stool)-like diarrhea, listlessness, loss of appetite, Eyes closed and feathers ruffled. Autopsy of dead or severely ill chickens reveals gas-filled intestines with a fragile intestinal wall containing a foul-smelling brown fluid and clearly visible necrotic lesions on the intestinal wall. At the cellular level, the intestines of poultry with necrotizing enteritis were inflamed—scattered and clustered necrosis on the villi and crypts, immune cells infiltrated the lamina propria, and had crypt abscesses. [0003] At present, the most commonly used drugs to treat po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K10/16A23K20/105
CPCA23K50/75A23K10/16A23K20/105
Inventor 王政张大伟闫舟梁晶晶袁江齐长海
Owner QINGDAO SHANGDE BIOTECH
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